Construct: sgRNA BRDN0001487047
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCTTCCTCAACCAAAGTGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK7 (122481)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77912
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96398181 |
+ |
AK7 |
NNNTTCCTCAACCAAAGTGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
82127573 |
+ |
CCDC57 |
NNNTTACTCAACCAAAGTGC |
NGG |
2 |
0.211 |
Tier I |
| 3 |
NC_000001.11 |
1 |
18841734 |
- |
TAS1R2 |
NNNTTCCTCAACCACACTGA |
NGG |
2 |
0.0642 |
Tier I |
| 4 |
NC_000001.11 |
1 |
47348511 |
- |
CMPK1 |
NNNTTCCTTTACCAAAGTGA |
NGG |
2 |
0.7721 |
Tier II |
| 5 |
NC_000003.12 |
3 |
1360039 |
- |
CNTN6 |
NNNTTAATCAACCAAAGTGA |
NGG |
2 |
0.6964 |
Tier II |
| 6 |
NC_000005.10 |
5 |
95412881 |
+ |
FAM81B |
NNNTTCCTCAGCCAAAGTGA |
NGG |
1 |
0.65 |
Tier II |
| 7 |
NC_000015.10 |
15 |
66740832 |
- |
SMAD6 |
NNNTTCCCCAACCAAAGTGT |
NGG |
2 |
0.6 |
Tier II |
| 8 |
NC_000006.12 |
6 |
16563190 |
+ |
ATXN1 |
NNNGTCCTCTACCAAAGTGA |
NGG |
2 |
0.5515 |
Tier II |
| 9 |
NC_000002.12 |
2 |
50109749 |
- |
NRXN1 |
NNNGGCCTCAACCAAAGTGA |
NGG |
2 |
0.4 |
Tier II |
| 10 |
NC_000015.10 |
15 |
49284046 |
+ |
GALK2 |
NNNTTCCTCAACAAAAGTGA |
NGG |
1 |
0.3846 |
Tier II |
| 11 |
NC_000015.10 |
15 |
77149075 |
- |
PEAK1 |
NNNTTCCTCATCCAAAATGA |
NGG |
2 |
0.2872 |
Tier II |
| 12 |
NC_000003.12 |
3 |
21943712 |
+ |
ZNF385D |
NNNTTTCTCAACCAAAGTGA |
NAG |
2 |
0.2407 |
Tier II |
| 13 |
NC_000015.10 |
15 |
91952931 |
- |
SLCO3A1 |
NNNTTCCTCAACCAAGGTGA |
NGG |
1 |
0.1923 |
Tier II |
| 14 |
NC_000002.12 |
2 |
31984109 |
+ |
MEMO1 |
NNNTTCCTCATCCAAAGTGT |
NGG |
2 |
0.1846 |
Tier II |
| 15 |
NC_000016.10 |
16 |
73665603 |
+ |
ZFHX3 |
NNNTTGCTCCACCAAAGTGA |
NGG |
2 |
0.1667 |
Tier II |
| 16 |
NC_000007.14 |
7 |
158440391 |
+ |
PTPRN2 |
NNNTTCCTCAACAACAGTGA |
NGG |
2 |
0.1049 |
Tier II |
| 17 |
NC_000011.10 |
11 |
40740398 |
- |
LRRC4C |
NNNTTCCTCAACAACAGTGA |
NGG |
2 |
0.1049 |
Tier II |
| 18 |
NC_000013.11 |
13 |
91607372 |
+ |
GPC5 |
NNNTTCCTCAAGCAAACTGA |
NGG |
2 |
0.1046 |
Tier II |
| 19 |
NC_000018.10 |
18 |
47217349 |
- |
SKOR2 |
NNNTTCCTCAACCAAAATGA |
NCG |
2 |
0.1 |
Tier II |
| 20 |
NC_000001.11 |
1 |
18707865 |
- |
PAX7 |
NNNTTCCTCCACCAAACTGA |
NGG |
2 |
0.0784 |
Tier II |
| 21 |
NC_000004.12 |
4 |
6604750 |
- |
MAN2B2 |
NNNTTCATCAACCAAAGTGA |
NGA |
2 |
0.0521 |
Tier II |
| 22 |
NC_000016.10 |
16 |
70317462 |
+ |
DDX19B |
NNNTTCCTCAACCAAAGAGA |
NTG |
2 |
0.026 |
Tier II |
| 23 |
NC_000017.11 |
17 |
66788048 |
- |
PRKCA |
NNNTTCCTCAACCAAAGTGT |
NTG |
2 |
0.0234 |
Tier II |
| 24 |
NC_000011.10 |
11 |
113211094 |
- |
NCAM1 |
NNNTTCCTCAACCAATGGGA |
NGG |
2 |
0.0 |
Tier II |
| 25 |
NC_000007.14 |
7 |
28197464 |
- |
JAZF1-AS1 |
NNNTTCCTCAAGCAAAATGA |
NGG |
2 |
0.4148 |
Tier III |
| 26 |
NC_000001.11 |
1 |
98277935 |
- |
LOC124900404 |
NNNTTCCTGAACCAAACTGA |
NGG |
2 |
0.1457 |
Tier III |
| 27 |
NC_000002.12 |
2 |
150242370 |
+ |
LINC01817 |
NNNTTCCTCAACCCAAGTGA |
NAG |
2 |
0.0556 |
Tier III |
| 28 |
NC_000002.12 |
2 |
150242370 |
+ |
LINC01818 |
NNNTTCCTCAACCCAAGTGA |
NAG |
2 |
0.0556 |
Tier III |
| 29 |
NC_000016.10 |
16 |
70317462 |
+ |
DDX19A-DT |
NNNTTCCTCAACCAAAGAGA |
NTG |
2 |
0.026 |
Tier III |
| 30 |
NC_000021.9 |
21 |
18743082 |
- |
MIR548XHG |
NNNTTCCTCAAACAATGTGA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
33406673 |
+ |
Ranbp17 |
NNNTTCCTCAACCAAAATGG |
NGG |
2 |
0.7137 |
Tier II |
| 2 |
NC_000067.6 |
1 |
21920675 |
+ |
Kcnq5 |
NNNTTACTGAACCAAAGTGA |
NGG |
2 |
0.5748 |
Tier II |
| 3 |
NC_000069.6 |
3 |
116503489 |
- |
Rtca |
NNNTTCCAGAACCAAAGTGA |
NGG |
2 |
0.4952 |
Tier II |
| 4 |
NC_000083.6 |
17 |
64834926 |
+ |
4930583I09Rik |
NNNTTCCTAAATCAAAGTGA |
NGG |
2 |
0.4615 |
Tier II |
| 5 |
NC_000072.6 |
6 |
83873341 |
- |
Zfp638 |
NNNTGCCTCAAACAAAGTGA |
NGG |
2 |
0.4571 |
Tier II |
| 6 |
NC_000082.6 |
16 |
33962795 |
+ |
Umps |
NNNTACCTCAAACAAAGTGA |
NGG |
2 |
0.3571 |
Tier II |
| 7 |
NC_000071.6 |
5 |
150432398 |
+ |
Fry |
NNNTTTCTCAACCAAAGGGA |
NGG |
2 |
0.3095 |
Tier II |
| 8 |
NC_000073.6 |
7 |
99699034 |
+ |
Slco2b1 |
NNNATCCTCAACCAAAGTGT |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000072.6 |
6 |
43625021 |
- |
Tpk1 |
NNNTTCCTCATTCAAAGTGA |
NGG |
2 |
0.1657 |
Tier II |
| 10 |
NC_000074.6 |
8 |
114834623 |
+ |
Wwox |
NNNTGCCTCAACCAAACTGA |
NGG |
2 |
0.1506 |
Tier II |
| 11 |
NC_000084.6 |
18 |
61641155 |
+ |
Bvht |
NNNTTCCTTAGCCAAAGTGA |
NGG |
2 |
0.5688 |
Tier III |
| 12 |
NC_000072.6 |
6 |
31238291 |
+ |
2210408F21Rik |
NNNTTCCTCGACCAAAGAGA |
NGG |
2 |
0.3704 |
Tier III |
| 13 |
NC_000073.6 |
7 |
99699034 |
+ |
Gm34280 |
NNNATCCTCAACCAAAGTGT |
NGG |
2 |
0.2857 |
Tier III |
| 14 |
NC_000074.6 |
8 |
72280297 |
+ |
Gm51537 |
NNNTTCCACATCCAAAGTGA |
NGG |
2 |
0.2462 |
Tier III |
Other clones with same target sequence:
(none)
