Construct: sgRNA BRDN0001487050
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCAAGTACTGCATTCCCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ZAP70 (7535)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77956
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
97732991 |
+ |
ZAP70 |
NNNAAGTACTGCATTCCCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
110087837 |
+ |
ADD3 |
NNNAAGTACTGCAATCCCGG |
NGG |
2 |
0.4734 |
Tier II |
| 3 |
NC_000001.11 |
1 |
92148912 |
- |
BTBD8 |
NNNGAGTACTGCATTCCAGA |
NGG |
2 |
0.3365 |
Tier II |
| 4 |
NC_000001.11 |
1 |
75289508 |
+ |
SLC44A5 |
NNNAAGTACTCCATTCCCAA |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000005.10 |
5 |
137650452 |
- |
KLHL3 |
NNNAAGTACTGGATTCACGA |
NGG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000018.10 |
18 |
36212875 |
- |
MOCOS |
NNNAAGTACTCCATTCCCCA |
NGG |
2 |
0.1921 |
Tier II |
| 7 |
NC_000010.11 |
10 |
35060422 |
- |
CUL2 |
NNNAAGTTCTGCATTCCCCA |
NGG |
2 |
0.1921 |
Tier II |
| 8 |
NC_000018.10 |
18 |
46739728 |
- |
ST8SIA5 |
NNNAAGTCCTTCATTCCCGA |
NGG |
2 |
0.1648 |
Tier II |
| 9 |
NC_000001.11 |
1 |
115726122 |
+ |
CASQ2 |
NNNAAGTACTGCCTTCCTGA |
NGG |
2 |
0.1353 |
Tier II |
| 10 |
NC_000012.12 |
12 |
119809007 |
- |
CIT |
NNNAAGTACTGCATTCCCCA |
NTG |
2 |
0.0175 |
Tier II |
| 11 |
NC_000006.12 |
6 |
13423057 |
+ |
GFOD1 |
NNNAAGTACTGCATGTCCGA |
NGG |
2 |
0.0154 |
Tier II |
| 12 |
NC_000001.11 |
1 |
166694936 |
+ |
FMO10P |
NNNAAGTACTGAATTCCCAA |
NGG |
2 |
0.5102 |
Tier III |
| 13 |
NC_000023.11 |
X |
88514578 |
- |
LOC107985713 |
NNNAAGTATTGCATTCCAGA |
NGG |
2 |
0.4712 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
36778261 |
+ |
Zap70 |
NNNAAGTACTGCATCCCCGA |
NGG |
1 |
0.2727 |
Tier I |
| 2 |
NC_000067.6 |
1 |
188356803 |
+ |
Ush2a |
NNNCAGTACTGCATTCCCAA |
NGG |
2 |
0.2521 |
Tier I |
| 3 |
NC_000072.6 |
6 |
80722937 |
+ |
Lrrtm4 |
NNNAAGTACTGCATACCAGA |
NGG |
2 |
0.3117 |
Tier II |
| 4 |
NC_000079.6 |
13 |
19161831 |
- |
Gm31887 |
NNNAATTACTGCATTCCCCA |
NGG |
2 |
0.2989 |
Tier III |
| 5 |
NC_000083.6 |
17 |
69906636 |
+ |
Gm36594 |
NNNAAGGACTGCAGTCCCGA |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
