Construct: sgRNA BRDN0001487051
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATTGAGAACTCGAAGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LRGUK (136332)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78086
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
134174601 |
- |
LRGUK |
NNNTTGAGAACTCGAAGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
107279350 |
+ |
ANGPT1 |
NNNTTGAGAACTGGAAAGAT |
NGG |
2 |
0.1273 |
Tier II |
| 3 |
NC_000013.11 |
13 |
70066192 |
- |
KLHL1 |
NNNTTGAGAACTCGAAGCAT |
NAG |
2 |
0.1235 |
Tier II |
| 4 |
NC_000005.10 |
5 |
145602721 |
+ |
PRELID2 |
NNNTTGAGAACTCAATGGAT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000006.12 |
6 |
97691191 |
+ |
LOC101927314 |
NNNTTGAGAACTCAAAGAAT |
NGG |
2 |
0.5192 |
Tier III |
| 6 |
NC_000005.10 |
5 |
89262572 |
- |
MEF2C-AS1 |
NNNTTGAGAACTTGAAGGTT |
NGG |
2 |
0.3769 |
Tier III |
| 7 |
NC_000008.11 |
8 |
78436827 |
+ |
LOC105375911 |
NNNTTGAGAACTCGGAGGAA |
NGG |
2 |
0.3656 |
Tier III |
| 8 |
NC_000006.12 |
6 |
145836200 |
+ |
EPM2A-DT |
NNNTTGAGGACTCTAAGGAT |
NGG |
2 |
0.1778 |
Tier III |
| 9 |
NC_000008.11 |
8 |
102621364 |
- |
POU5F1P2 |
NNNTTGAGAACACGAAGGAT |
NGA |
2 |
0.0556 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
34071106 |
- |
Lrguk |
NNNTTGAGAACTCGAAGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000071.6 |
5 |
102643615 |
+ |
Arhgap24 |
NNNCTGAGAACTCGAAGGTT |
NGG |
2 |
0.3484 |
Tier II |
| 3 |
NC_000067.6 |
1 |
179474677 |
- |
Smyd3 |
NNNTTGAGAACAAGAAGGAT |
NGG |
2 |
0.3077 |
Tier II |
| 4 |
NC_000068.7 |
2 |
37493805 |
- |
Rabgap1 |
NNNTTGAGAACTAGAATGAT |
NGG |
2 |
0.0962 |
Tier II |
| 5 |
NC_000081.6 |
15 |
93571744 |
+ |
Prickle1 |
NNNTTGAGAACTCGAAGCAG |
NGG |
2 |
0.084 |
Tier II |
| 6 |
NC_000075.6 |
9 |
75463791 |
- |
Leo1 |
NNNATGAGAACTGGAAGGAT |
NGG |
2 |
0.0649 |
Tier II |
| 7 |
NC_000071.6 |
5 |
127461935 |
- |
Tmem132c |
NNNTTGAGAACTGGAAGGAT |
NAG |
2 |
0.0354 |
Tier II |
| 8 |
NC_000073.6 |
7 |
130705489 |
- |
Tacc2 |
NNNTTGAGAACTCGAAGCAT |
NGA |
2 |
0.0331 |
Tier II |
| 9 |
NC_000076.6 |
10 |
81284526 |
- |
Tjp3 |
NNNTTGAGAAATCGATGGAT |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
