Construct: sgRNA BRDN0001487057

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTCATCGAGGAAAAACAGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PINK1 (65018)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78037

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 20633892 + PINK1 NNNCATCGAGGAAAAACAGG NGG 0 1.0 Tier I
2 NC_000020.11 20 44639227 + ADA NNNCATAGAGGAAAAACAGG NGG 1 0.75 Tier II
3 NC_000008.11 8 107400491 + ANGPT1 NNNCAACTAGGAAAAACAGG NGG 2 0.5417 Tier II
4 NC_000005.10 5 179561625 + RUFY1 NNNCATCAAGGAAAAAAAGG NGG 2 0.4667 Tier II
5 NC_000001.11 1 151238634 + PIP5K1A NNNCAAGGAGGAAAAACAGG NGG 2 0.4078 Tier II
6 NC_000001.11 1 241027759 + RGS7 NNNTATGGAGGAAAAACAGG NGG 2 0.3765 Tier II
7 NC_000011.10 11 8867455 - DENND2B NNNTATGGAGGAAAAACAGG NGG 2 0.3765 Tier II
8 NC_000006.12 6 166506593 - RPS6KA2 NNNCATGGAGGAAAAACAGT NGG 2 0.3294 Tier II
9 NC_000002.12 2 230874262 - ITM2C NNNCATCGTGGAAAAACTGG NGG 2 0.3 Tier II
10 NC_000005.10 5 133540473 - FSTL4 NNNCATCCAGGAAAAAAAGG NGG 2 0.2872 Tier II
11 NC_000010.11 10 98254608 + LOXL4 NNNCAGGGAGGAAAAACAGG NGG 2 0.2689 Tier II
12 NC_000008.11 8 23387479 + LOXL2 NNNCTTCCAGGAAAAACAGG NGG 2 0.2238 Tier II
13 NC_000008.11 8 28095414 - ELP3 NNNCATGGAGGAAAAAAAGG NGG 2 0.2196 Tier II
14 NC_000002.12 2 133245715 + NCKAP5 NNNCATAGAGGAAAAACAGG NGA 2 0.0521 Tier II
15 NC_000017.11 17 17542616 + PEMT NNNCATGGAGGAAAAACAGG NGA 2 0.0327 Tier II
16 NC_000003.12 3 147392670 + ZIC4 NNNCATCCAGGAAAAACAGG NGC 2 0.0137 Tier II
17 NC_000004.12 4 44277629 - KCTD8 NNNTATCGAGGAAAAACAGG NGT 2 0.0129 Tier II
18 NC_000012.12 12 2381582 - CACNA1C NNNCATCGAGGAAAACCTGG NGG 2 0.0 Tier II
19 NC_000008.11 8 62252608 - NKAIN3 NNNCATCTAGGAAAATCAGG NGG 2 0.0 Tier II
20 NC_000002.12 2 105964458 - LOC105373531 NNNCATGGAGGAAAAACAGG NGG 1 0.4706 Tier III
21 NC_000005.10 5 179561625 + LOC128966623 NNNCATCAAGGAAAAAAAGG NGG 2 0.4667 Tier III
22 NC_000006.12 6 90932891 + LOC107986623 NNNCATCTAGGAAAAACAGT NGG 2 0.4375 Tier III
23 NC_000013.11 13 110106756 + LINC03082 NNNCATCGATGAATAACAGG NGG 2 0.4333 Tier III
24 NC_000001.11 1 50027927 + LOC105378708 NNNCATCTAGTAAAAACAGG NGG 2 0.2404 Tier III
25 NC_000008.11 8 62252608 - LOC124901952 NNNCATCTAGGAAAATCAGG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 55421772 + Vti1a NNNCATCGATGAAAGACAGG NGG 2 0.5281 Tier II
2 NC_000072.6 6 89414580 + Chchd6 NNNCATCGAGGAGAAACAGT NGG 2 0.4565 Tier II
3 NC_000077.6 11 115489219 + Armc7 NNNCATCCAGGGAAAACAGG NGG 2 0.4444 Tier II
4 NC_000077.6 11 7201584 + Igfbp1 NNNCATTGAGGAAAAACTGG NGG 2 0.4063 Tier II
5 NC_000072.6 6 89414036 - Chchd6 NNNCATTGAGGAAAAACAGC NGG 2 0.3482 Tier II
6 NC_000078.6 12 53038719 + Akap6 NNNCATCTAGGAAAAAAAGG NGG 2 0.2917 Tier II
7 NC_000070.6 4 4791599 + Bpnt2 NNNCATGGAGGAAAAAAAGG NGG 2 0.2196 Tier II
8 NC_000070.6 4 62459046 - Wdr31 NNNCACCGAGGAAAAGCAGG NGG 2 0.1748 Tier II
9 NC_000071.6 5 102776201 + Arhgap24 NNNCCTCGAGGAAAAACAGG NAG 2 0.1296 Tier II
10 NC_000072.6 6 96476639 + Tafa1 NNNCCTCGAGGAAAAACAGG NGC 2 0.0111 Tier II
11 NC_000067.6 1 52344868 + Gm31955 NNNTATTGAGGAAAAACAGG NGG 2 0.65 Tier III
12 NC_000082.6 16 23181527 + C730014E05Rik NNNCATGGAAGAAAAACAGG NGG 2 0.4392 Tier III
13 NC_000080.6 14 104678283 + D130009I18Rik NNNCATTGAGGCAAAACAGG NGG 2 0.2138 Tier III
14 NC_000068.7 2 163145617 - Gm11454 NNNCATCAAGGAAAAACAGG NGT 2 0.0161 Tier III
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Other clones with same target sequence:

(none)