Construct: sgRNA BRDN0001487058
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCGGGGACCACCCACGATCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- Negative control (unspec'd)
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80211
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000016.10 |
16 |
67396032 |
+ |
ZDHHC1 |
NNNAGGACCAGCCACGATCC |
NGG |
2 |
0.225 |
Tier II |
| 2 |
NC_000020.11 |
20 |
16284883 |
+ |
KIF16B |
NNNGGGAACACCCACGGTCC |
NGG |
2 |
0.1147 |
Tier II |
| 3 |
NC_000017.11 |
17 |
38580717 |
- |
SRCIN1 |
NNNGGGACCACCCAAGACCC |
NGG |
2 |
0.0952 |
Tier II |
| 4 |
NC_000017.11 |
17 |
82520403 |
+ |
FOXK2 |
NNNGGGACCACCCACGAGCG |
NGG |
2 |
0.0196 |
Tier II |
| 5 |
NC_000002.12 |
2 |
23475456 |
+ |
KLHL29 |
NNNGGGACCACCCAGGATGC |
NGG |
2 |
0.0063 |
Tier II |
| 6 |
NC_000002.12 |
2 |
159206162 |
+ |
TANC1 |
NNNGGGACCACCCAGGATCC |
NGA |
2 |
0.0035 |
Tier II |
| 7 |
NC_000004.12 |
4 |
2370652 |
+ |
ZFYVE28 |
NNNGGGAGCACCCACCATCC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000007.14 |
7 |
135316880 |
- |
LOC107984123 |
NNNGGGACCACCCACGATCC |
NGG |
0 |
1.0 |
Tier III |
| 9 |
NC_000002.12 |
2 |
23475456 |
+ |
LOC124907741 |
NNNGGGACCACCCAGGATGC |
NGG |
2 |
0.0063 |
Tier III |
| 10 |
NC_000022.11 |
22 |
22810908 |
+ |
IGL |
NNNGGGACCACCCACCATCC |
NGA |
2 |
0.0 |
Tier III |
| 11 |
NC_000004.12 |
4 |
2370652 |
+ |
LOC107986249 |
NNNGGGAGCACCCACCATCC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
112658208 |
+ |
Ryr3 |
NNNGGGACCTCCCACAATCC |
NGG |
2 |
0.8824 |
Tier II |
| 2 |
NC_000074.6 |
8 |
57797945 |
- |
Galntl6 |
NNNGGTACCACCCACAATCC |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000068.7 |
2 |
150947540 |
+ |
Ninl |
NNNGTGACCACCCACAATCC |
NGG |
2 |
0.3 |
Tier II |
| 4 |
NC_000072.6 |
6 |
121326306 |
- |
Slc6a13 |
NNNGGGACCATCCAAGATCC |
NGG |
2 |
0.0684 |
Tier II |
| 5 |
NC_000073.6 |
7 |
136966728 |
- |
Mgmt |
NNNGGGACCAGCCAAGATCC |
NGG |
2 |
0.0556 |
Tier II |
| 6 |
NC_000073.6 |
7 |
44904199 |
- |
Ap2a1 |
NNNGGGACCACCCACCCTCC |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000081.6 |
15 |
97069855 |
+ |
Gm32840 |
NNNGGTACCACCCACAATCC |
NGG |
2 |
0.6667 |
Tier III |
| 8 |
NC_000077.6 |
11 |
22532249 |
+ |
Gm51939 |
NNNGGGACCCCCCAGGATCC |
NGG |
2 |
0.0167 |
Tier III |
Other clones with same target sequence:
(none)
