Construct: sgRNA BRDN0001487060

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTGCCCACCTAAGCTTACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MST1R (4486)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78050

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 49898523 - MST1R NNNCCCACCTAAGCTTACTG NGG 0 1.0 Tier I
2 NC_000018.10 18 79472307 - NFATC1 NNNCCCAGCTGAGCTTACTG NGG 2 0.4179 Tier II
3 NC_000012.12 12 39746462 + REDIC1 NNNCCCACCTCAGCTCACTG NGG 2 0.2667 Tier II
4 NC_000010.11 10 116757168 - HSPA12A NNNCCCAGCTAAGCCTACTG NGG 2 0.1753 Tier II
5 NC_000003.12 3 9768605 + OGG1 NNNCCCAGCTAAGCTTGCTG NGG 2 0.1134 Tier II
6 NC_000003.12 3 9768605 + CAMK1 NNNCCCAGCTAAGCTTGCTG NGG 2 0.1134 Tier II
7 NC_000007.14 7 98396193 + BAIAP2L1 NNNCCCACCTCAGCCTACTG NGG 2 0.1091 Tier II
8 NC_000006.12 6 143716597 + PHACTR2 NNNCCCACCTCAGCTTACTG NAG 2 0.1037 Tier II
9 NC_000002.12 2 61103260 + SANBR NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
10 NC_000003.12 3 155618769 + PLCH1 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
11 NC_000006.12 6 27460062 - ZNF184 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
12 NC_000010.11 10 12636451 + CAMK1D NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
13 NC_000010.11 10 74192650 + ADK NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
14 NC_000012.12 12 122365904 - CLIP1 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
15 NC_000013.11 13 30563504 - HMGB1 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
16 NC_000013.11 13 92102982 + GPC5 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
17 NC_000014.9 14 62107328 - SYT16 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
18 NC_000015.10 15 68976295 + SPESP1-NOX5 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
19 NC_000019.10 19 47207758 + SAE1 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
20 NC_000022.11 22 33209769 + LARGE1 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier II
21 NC_000010.11 10 25420273 + GPR158 NNNCCCACCTCAGCTTTCTG NGG 2 0.0533 Tier II
22 NC_000011.10 11 11892711 - USP47 NNNCCCACCTAAGCCTCCTG NGG 2 0.0481 Tier II
23 NC_000009.12 9 36121008 - RECK NNNCCCACCTATGCTTACTG NGC 2 0.0074 Tier II
24 NC_000003.12 3 13119532 - IQSEC1 NNNCCCACCTAACCTTACTG NGT 2 0.0068 Tier II
25 NC_000002.12 2 73868769 - STAMBP NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
26 NC_000010.11 10 104704291 + SORCS3 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
27 NC_000012.12 12 11147311 + PRH1 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
28 NC_000012.12 12 11147311 + PRH1-TAS2R14 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
29 NC_000015.10 15 42463695 - ZNF106 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
30 NC_000015.10 15 51845652 - TMOD3 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
31 NC_000023.11 X 53119171 + KANTR NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier II
32 NC_000003.12 3 81631099 - GBE1 NNNCCCACCTAAGCCTACTG NGT 2 0.0044 Tier II
33 NC_000014.9 14 20347997 + PARP2 NNNCCCACCTAAGCTTCCTG NGT 2 0.0028 Tier II
34 NC_000017.11 17 74691350 - RAB37 NNNCCCATCTAAGGTTACTG NGG 2 0.0 Tier II
35 NC_000018.10 18 79472307 - LOC107985162 NNNCCCAGCTGAGCTTACTG NGG 2 0.4179 Tier III
36 NC_000006.12 6 29331766 + LOC105375005 NNNCCCACCTCAGCATACTG NGG 2 0.2316 Tier III
37 NC_000006.12 6 131429785 - LOC105378005 NNNCCCACCTCAGCCTACTG NGG 2 0.1091 Tier III
38 NC_000004.12 4 121957958 - LOC102724158 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier III
39 NC_000008.11 8 85229701 - LOC105375936 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier III
40 NC_000008.11 8 85229701 - LOC124901969 NNNCCCACCTCAGCTTCCTG NGG 2 0.0706 Tier III
41 NC_000012.12 12 87790607 - LINC02258 NNNCCCACCTAACCTTACTG NGT 2 0.0068 Tier III
42 NC_000012.12 12 11147311 + PRH1-PRR4 NNNCCCACCTCAGCTTACTG NGT 2 0.0065 Tier III
43 NC_000003.12 3 66713446 - LOC105377142 NNNCCCACCTAAGCTTCCTG NGT 2 0.0028 Tier III
44 NC_000004.12 4 84536561 - LOC102723338 NNNCCCACCTAAGCTTCCTG NGT 2 0.0028 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 61157036 - Usp22 NNNCTTACCTAAGCTTACTG NGG 2 0.5909 Tier II
2 NC_000079.6 13 85198023 - Ccnh NNNCCCACCTAAACTTCCTG NGG 2 0.1629 Tier II
3 NC_000068.7 2 179761330 + Cdh4 NNNCCCCCCTAAGCTGACTG NGG 2 0.1514 Tier II
4 NC_000073.6 7 145252959 + Tpcn2 NNNCCCACCTACCCTTACTG NGG 2 0.1108 Tier II
5 NC_000069.6 3 83913199 + Tmem131l NNNCCCACCTAAGCCTACAG NGG 2 0.0779 Tier II
6 NC_000076.6 10 118556114 + Ifngas1 NNNTCCACCAAAGCTTACTG NGG 2 0.6857 Tier III
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Other clones with same target sequence:

(none)