Construct: sgRNA BRDN0001487061
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTGCCATGTGTACGACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALDH18A1 (5832)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77996
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
95643134 |
+ |
ALDH18A1 |
NNNTGCCATGTGTACGACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
179067208 |
- |
ZNF354C |
NNNTGACATGTGAACGACTG |
NGG |
2 |
0.6429 |
Tier II |
| 3 |
NC_000012.12 |
12 |
123692627 |
+ |
TCTN2 |
NNNGGCCATGTGTACGCCTG |
NGG |
2 |
0.1103 |
Tier II |
| 4 |
NC_000015.10 |
15 |
82197928 |
+ |
EFL1 |
NNNTGCCATGTGTAAGACTC |
NGG |
2 |
0.0952 |
Tier II |
| 5 |
NC_000009.12 |
9 |
9977647 |
+ |
PTPRD |
NNNTGCCATGAGTACGACTG |
NCG |
2 |
0.0804 |
Tier II |
| 6 |
NC_000019.10 |
19 |
5074972 |
- |
KDM4B |
NNNTGCCATGTGGACGACAG |
NGG |
2 |
0.0745 |
Tier II |
| 7 |
NC_000003.12 |
3 |
113602138 |
- |
SIDT1 |
NNNTGCCATGTGAACGACTG |
NGT |
2 |
0.0112 |
Tier II |
| 8 |
NC_000005.10 |
5 |
112353605 |
- |
EPB41L4A |
NNNTGCCATGTGTACCACTG |
NTG |
2 |
0.0 |
Tier II |
| 9 |
NC_000009.12 |
9 |
88923179 |
- |
LOC124902205 |
NNNTGCCATGTGTACGACTA |
NGT |
2 |
0.0151 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
32448513 |
- |
Chat |
NNNTCCCCTGTGTACGACTG |
NGG |
2 |
0.3367 |
Tier II |
| 2 |
NC_000085.6 |
19 |
40696300 |
- |
Entpd1 |
NNNTGCCATGTGTGCGACGG |
NGG |
2 |
0.1167 |
Tier II |
| 3 |
NC_000071.6 |
5 |
65244073 |
- |
Wdr19 |
NNNTGCCAAGTGTATGACTG |
NGG |
2 |
0.0619 |
Tier II |
| 4 |
NC_000073.6 |
7 |
67985045 |
- |
Igf1r |
NNNTGCCATGTGTAGGAATG |
NGG |
2 |
0.0269 |
Tier II |
| 5 |
NC_000078.6 |
12 |
82426677 |
+ |
Sipa1l1 |
NNNTGCCTTGTGTAGGACTG |
NGG |
2 |
0.0214 |
Tier II |
| 6 |
NC_000077.6 |
11 |
46352056 |
+ |
Itk |
NNNTGCCATGTGTATGAGTG |
NGG |
2 |
0.0089 |
Tier II |
| 7 |
NC_000087.7 |
Y |
928794 |
- |
Kdm5d |
NNNTGCCATGTGTATGTCTG |
NGG |
2 |
0.0089 |
Tier II |
| 8 |
NC_000073.6 |
7 |
104447037 |
- |
Trim30a |
NNNTGCCATGTGTCCTACTG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000083.6 |
17 |
52858163 |
+ |
Kcnh8 |
NNNTGTCATGTGTACTACTG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000075.6 |
9 |
31562530 |
- |
Gm31408 |
NNNTGCCATGTGTCCGACTG |
NTG |
2 |
0.0083 |
Tier III |
| 11 |
NC_000075.6 |
9 |
23064216 |
+ |
Gm36743 |
NNNTGCCATGTGTACTGCTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
