Construct: sgRNA BRDN0001487062

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGACACAATATGAGAACC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGI1 (9223)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78068

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 65439984 - MAGI1 NNNGACACAATATGAGAACC NGG 0 1.0 Tier I
2 NC_000003.12 3 108078061 - CD47 NNNGACACAATATGAAAATC NGG 2 0.4615 Tier II
3 NC_000005.10 5 66166287 - SREK1 NNNAACACAATATGAGATCC NGG 2 0.45 Tier II
4 NC_000012.12 12 130376871 - PIWIL1 NNNGACACAACATGGGAACC NGG 2 0.4333 Tier II
5 NC_000002.12 2 207582622 - CREB1 NNNAACACAAGATGAGAACC NGG 2 0.36 Tier II
6 NC_000002.12 2 207582622 - METTL21A NNNAACACAAGATGAGAACC NGG 2 0.36 Tier II
7 NC_000006.12 6 100743288 + ASCC3 NNNGTCACAATGTGAGAACC NGG 2 0.2626 Tier II
8 NC_000023.11 X 71985437 - NHSL2 NNNGACACAATTTGAGAAAC NGG 2 0.1429 Tier II
9 NC_000005.10 5 72838590 - TNPO1 NNNGACAAAATATGAGCACC NGG 2 0.1147 Tier II
10 NC_000010.11 10 13070401 + CCDC3 NNNGACACAATTGGAGAACC NGG 2 0.087 Tier II
11 NC_000006.12 6 80367738 - BCKDHB NNNGACTCAATATGAGTACC NGG 2 0.0583 Tier II
12 NC_000011.10 11 3130238 - OSBPL5 NNNGACACAAGATGAGAAGC NGG 2 0.05 Tier II
13 NC_000002.12 2 186079460 + LINC01473 NNNAATACAATATGAGAACC NGG 2 0.8357 Tier III
14 NC_000023.11 X 145845526 + LOC107985703 NNNGACAGAATATGAAAACC NGG 2 0.6429 Tier III
15 NC_000009.12 9 62881752 + LERFS NNNAACACAATATGAGATCC NGG 2 0.45 Tier III
16 NC_000004.12 4 600643 + LOC124900162 NNNGTCCCAATATGAGAACC NGG 2 0.1591 Tier III
17 NC_000012.12 12 64966316 + LINC02389 NNNGTCCCAATATGAGAACC NGG 2 0.1591 Tier III
18 NC_000012.12 12 64966316 + LINC02231 NNNGTCCCAATATGAGAACC NGG 2 0.1591 Tier III
19 NC_000005.10 5 164970215 - LINC03000 NNNGACACAATTTGAGAAAC NGG 2 0.1429 Tier III
20 NC_000023.11 X 119314852 + LINC03098 NNNGACACAATTTGAGAAAC NGG 2 0.1429 Tier III
21 NC_000002.12 2 131989905 + LINC01945 NNNGACACAATTTGAGAACC NAG 2 0.0864 Tier III
22 NC_000013.11 13 18544735 - LINC00349 NNNGACACAATTTGAGAACC NAG 2 0.0864 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 101625553 - Gm5414 NNNGACACAATATGAGAACA NTG 2 0.0195 Tier I
2 NC_000084.6 18 69651194 - Tcf4 NNNGACAGAATATGAAAACC NGG 2 0.6429 Tier II
3 NC_000070.6 4 77378779 - Ptprd NNNGACACAGTATAAGAACC NGG 2 0.4167 Tier II
4 NC_000082.6 16 16567327 + Fgd4 NNNAACTCAATATGAGAACC NGG 2 0.3938 Tier II
5 NC_000082.6 16 89992785 + Tiam1 NNNGGCACAAGATGAGAACC NGG 2 0.288 Tier II
6 NC_000085.6 19 31215035 + Prkg1 NNNAACACAATATGAGAACT NGG 2 0.27 Tier II
7 NC_000074.6 8 14887834 + Cln8 NNNGAGACAATATCAGAACC NGG 2 0.2143 Tier II
8 NC_000073.6 7 115990910 + Sox6 NNNGACACAGTTTGAGAACC NGG 2 0.1852 Tier II
9 NC_000072.6 6 65065052 - Smarcad1 NNNGTGACAATATGAGAACC NGG 2 0.1818 Tier II
10 NC_000078.6 12 36236248 + Lrrc72 NNNTTCACAATATGAGAACC NGG 2 0.1322 Tier II
11 NC_000072.6 6 52596293 - Hibadh NNNGACACAATATGAGAACA NAG 2 0.1296 Tier II
12 NC_000070.6 4 149763563 - Slc25a33 NNNGACACAATATGAGGAAC NGG 2 0.0756 Tier II
13 NC_000074.6 8 70868880 + Ccdc124 NNNGACAGAATATGAGAACG NGG 2 0.0378 Tier II
14 NC_000072.6 6 96461719 - Tafa1 NNNGACACAGTATGAGAACC NTG 2 0.0216 Tier II
15 NC_000081.6 15 20762188 + Cdh12 NNNGACACAATATGAGAATC NTG 2 0.018 Tier II
16 NC_000072.6 6 50136110 + Mpp6 NNNGACACTATATGAGAACC NGT 2 0.0097 Tier II
17 NC_000080.6 14 87031695 + Diaph3 NNNGACACAAGATGAGAACC NGT 2 0.0065 Tier II
18 NC_000084.6 18 69651194 - Gm31439 NNNGACAGAATATGAAAACC NGG 2 0.6429 Tier III
19 NC_000085.6 19 31215035 + 8430431K14Rik NNNAACACAATATGAGAACT NGG 2 0.27 Tier III
20 NC_000086.7 X 17491968 - Gm39497 NNNGACACAGTAGGAGAACC NGG 2 0.1449 Tier III
21 NC_000075.6 9 106601358 + Gm39420 NNNGACACAATATGAGAATC NAG 2 0.1197 Tier III
22 NC_000080.6 14 52603129 + Tcra NNNGACACAATATAAGAACC NGA 2 0.0521 Tier III
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Other clones with same target sequence:

(none)