Construct: sgRNA BRDN0001487062
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAGACACAATATGAGAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAGI1 (9223)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78068
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000003.12 |
3 |
65439984 |
- |
MAGI1 |
NNNGACACAATATGAGAACC |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000003.12 |
3 |
108078061 |
- |
CD47 |
NNNGACACAATATGAAAATC |
NGG |
2 |
0.4615 |
Tier II |
3 |
NC_000005.10 |
5 |
66166287 |
- |
SREK1 |
NNNAACACAATATGAGATCC |
NGG |
2 |
0.45 |
Tier II |
4 |
NC_000012.12 |
12 |
130376871 |
- |
PIWIL1 |
NNNGACACAACATGGGAACC |
NGG |
2 |
0.4333 |
Tier II |
5 |
NC_000002.12 |
2 |
207582622 |
- |
CREB1 |
NNNAACACAAGATGAGAACC |
NGG |
2 |
0.36 |
Tier II |
6 |
NC_000002.12 |
2 |
207582622 |
- |
METTL21A |
NNNAACACAAGATGAGAACC |
NGG |
2 |
0.36 |
Tier II |
7 |
NC_000006.12 |
6 |
100743288 |
+ |
ASCC3 |
NNNGTCACAATGTGAGAACC |
NGG |
2 |
0.2626 |
Tier II |
8 |
NC_000023.11 |
X |
71985437 |
- |
NHSL2 |
NNNGACACAATTTGAGAAAC |
NGG |
2 |
0.1429 |
Tier II |
9 |
NC_000005.10 |
5 |
72838590 |
- |
TNPO1 |
NNNGACAAAATATGAGCACC |
NGG |
2 |
0.1147 |
Tier II |
10 |
NC_000010.11 |
10 |
13070401 |
+ |
CCDC3 |
NNNGACACAATTGGAGAACC |
NGG |
2 |
0.087 |
Tier II |
11 |
NC_000006.12 |
6 |
80367738 |
- |
BCKDHB |
NNNGACTCAATATGAGTACC |
NGG |
2 |
0.0583 |
Tier II |
12 |
NC_000011.10 |
11 |
3130238 |
- |
OSBPL5 |
NNNGACACAAGATGAGAAGC |
NGG |
2 |
0.05 |
Tier II |
13 |
NC_000002.12 |
2 |
186079460 |
+ |
LINC01473 |
NNNAATACAATATGAGAACC |
NGG |
2 |
0.8357 |
Tier III |
14 |
NC_000023.11 |
X |
145845526 |
+ |
LOC107985703 |
NNNGACAGAATATGAAAACC |
NGG |
2 |
0.6429 |
Tier III |
15 |
NC_000009.12 |
9 |
62881752 |
+ |
LERFS |
NNNAACACAATATGAGATCC |
NGG |
2 |
0.45 |
Tier III |
16 |
NC_000004.12 |
4 |
600643 |
+ |
LOC124900162 |
NNNGTCCCAATATGAGAACC |
NGG |
2 |
0.1591 |
Tier III |
17 |
NC_000012.12 |
12 |
64966316 |
+ |
LINC02389 |
NNNGTCCCAATATGAGAACC |
NGG |
2 |
0.1591 |
Tier III |
18 |
NC_000012.12 |
12 |
64966316 |
+ |
LINC02231 |
NNNGTCCCAATATGAGAACC |
NGG |
2 |
0.1591 |
Tier III |
19 |
NC_000005.10 |
5 |
164970215 |
- |
LINC03000 |
NNNGACACAATTTGAGAAAC |
NGG |
2 |
0.1429 |
Tier III |
20 |
NC_000023.11 |
X |
119314852 |
+ |
LINC03098 |
NNNGACACAATTTGAGAAAC |
NGG |
2 |
0.1429 |
Tier III |
21 |
NC_000002.12 |
2 |
131989905 |
+ |
LINC01945 |
NNNGACACAATTTGAGAACC |
NAG |
2 |
0.0864 |
Tier III |
22 |
NC_000013.11 |
13 |
18544735 |
- |
LINC00349 |
NNNGACACAATTTGAGAACC |
NAG |
2 |
0.0864 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000081.6 |
15 |
101625553 |
- |
Gm5414 |
NNNGACACAATATGAGAACA |
NTG |
2 |
0.0195 |
Tier I |
2 |
NC_000084.6 |
18 |
69651194 |
- |
Tcf4 |
NNNGACAGAATATGAAAACC |
NGG |
2 |
0.6429 |
Tier II |
3 |
NC_000070.6 |
4 |
77378779 |
- |
Ptprd |
NNNGACACAGTATAAGAACC |
NGG |
2 |
0.4167 |
Tier II |
4 |
NC_000082.6 |
16 |
16567327 |
+ |
Fgd4 |
NNNAACTCAATATGAGAACC |
NGG |
2 |
0.3938 |
Tier II |
5 |
NC_000082.6 |
16 |
89992785 |
+ |
Tiam1 |
NNNGGCACAAGATGAGAACC |
NGG |
2 |
0.288 |
Tier II |
6 |
NC_000085.6 |
19 |
31215035 |
+ |
Prkg1 |
NNNAACACAATATGAGAACT |
NGG |
2 |
0.27 |
Tier II |
7 |
NC_000074.6 |
8 |
14887834 |
+ |
Cln8 |
NNNGAGACAATATCAGAACC |
NGG |
2 |
0.2143 |
Tier II |
8 |
NC_000073.6 |
7 |
115990910 |
+ |
Sox6 |
NNNGACACAGTTTGAGAACC |
NGG |
2 |
0.1852 |
Tier II |
9 |
NC_000072.6 |
6 |
65065052 |
- |
Smarcad1 |
NNNGTGACAATATGAGAACC |
NGG |
2 |
0.1818 |
Tier II |
10 |
NC_000078.6 |
12 |
36236248 |
+ |
Lrrc72 |
NNNTTCACAATATGAGAACC |
NGG |
2 |
0.1322 |
Tier II |
11 |
NC_000072.6 |
6 |
52596293 |
- |
Hibadh |
NNNGACACAATATGAGAACA |
NAG |
2 |
0.1296 |
Tier II |
12 |
NC_000070.6 |
4 |
149763563 |
- |
Slc25a33 |
NNNGACACAATATGAGGAAC |
NGG |
2 |
0.0756 |
Tier II |
13 |
NC_000074.6 |
8 |
70868880 |
+ |
Ccdc124 |
NNNGACAGAATATGAGAACG |
NGG |
2 |
0.0378 |
Tier II |
14 |
NC_000072.6 |
6 |
96461719 |
- |
Tafa1 |
NNNGACACAGTATGAGAACC |
NTG |
2 |
0.0216 |
Tier II |
15 |
NC_000081.6 |
15 |
20762188 |
+ |
Cdh12 |
NNNGACACAATATGAGAATC |
NTG |
2 |
0.018 |
Tier II |
16 |
NC_000072.6 |
6 |
50136110 |
+ |
Mpp6 |
NNNGACACTATATGAGAACC |
NGT |
2 |
0.0097 |
Tier II |
17 |
NC_000080.6 |
14 |
87031695 |
+ |
Diaph3 |
NNNGACACAAGATGAGAACC |
NGT |
2 |
0.0065 |
Tier II |
18 |
NC_000084.6 |
18 |
69651194 |
- |
Gm31439 |
NNNGACAGAATATGAAAACC |
NGG |
2 |
0.6429 |
Tier III |
19 |
NC_000085.6 |
19 |
31215035 |
+ |
8430431K14Rik |
NNNAACACAATATGAGAACT |
NGG |
2 |
0.27 |
Tier III |
20 |
NC_000086.7 |
X |
17491968 |
- |
Gm39497 |
NNNGACACAGTAGGAGAACC |
NGG |
2 |
0.1449 |
Tier III |
21 |
NC_000075.6 |
9 |
106601358 |
+ |
Gm39420 |
NNNGACACAATATGAGAATC |
NAG |
2 |
0.1197 |
Tier III |
22 |
NC_000080.6 |
14 |
52603129 |
+ |
Tcra |
NNNGACACAATATAAGAACC |
NGA |
2 |
0.0521 |
Tier III |
Other clones with same target sequence:
(none)