Construct: sgRNA BRDN0001487063

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTCTCTGTCTGCAACACAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK17A (9263)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77915

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000007.14 7 43608286 - STK17A NNNCTCTGTCTGCAACACAC NGG 0 1.0 Tier I
2 NC_000017.11 17 42041174 + C17orf113 NNNCTCTGTGTGCAAGACAC NGG 2 0.0598 Tier I
3 NC_000009.12 9 98456628 + GABBR2 NNNCTCTGTCTGTAAAACAC NGG 2 0.7 Tier II
4 NC_000014.9 14 32663801 + AKAP6 NNNGTATGTCTGCAACACAC NGG 2 0.4643 Tier II
5 NC_000007.14 7 137958923 + CREB3L2 NNNATCTGTCTGCAACACAA NGG 2 0.4211 Tier II
6 NC_000012.12 12 27612282 + PPFIBP1 NNNCTCTCTCTGCAGCACAC NGG 2 0.4 Tier II
7 NC_000023.11 X 133326996 - GPC4 NNNCTATGTGTGCAACACAC NGG 2 0.3611 Tier II
8 NC_000021.9 21 36203319 - DOP1B NNNCTGTGGCTGCAACACAC NGG 2 0.3095 Tier II
9 NC_000001.11 1 174353774 + RABGAP1L NNNCTGTCTCTGCAACACAC NGG 2 0.3077 Tier II
10 NC_000002.12 2 109385621 - RANBP2 NNNCTCTGCCTGCAATACAC NGG 2 0.284 Tier II
11 NC_000002.12 2 109385621 - SH3RF3 NNNCTCTGCCTGCAATACAC NGG 2 0.284 Tier II
12 NC_000001.11 1 108251858 - NBPF4 NNNCTCTGTCTGCAACACTA NGG 2 0.2692 Tier II
13 NC_000001.11 1 108442514 + NBPF6 NNNCTCTGTCTGCAACACTA NGG 2 0.2692 Tier II
14 NC_000012.12 12 52945348 + KRT8 NNNCACTGTCTCCAACACAC NGG 2 0.2647 Tier II
15 NC_000010.11 10 69326398 - HK1 NNNTTCTGTCTGCAACACAC NAG 2 0.2074 Tier II
16 NC_000002.12 2 133367930 - NCKAP5 NNNCTTTGTCTGCATCACAC NGG 2 0.1857 Tier II
17 NC_000022.11 22 29220214 + EMID1 NNNCTCTGCCTGCAACCCAC NGG 2 0.1629 Tier II
18 NC_000008.11 8 20303249 + LZTS1 NNNCTCTGGCTGCAACGCAC NGG 2 0.1092 Tier II
19 NC_000013.11 13 39389662 - LHFPL6 NNNCACTGTCTGCCACACAC NGG 2 0.1071 Tier II
20 NC_000023.11 X 110794337 + CHRDL1 NNNCTCTGTCTGCTTCACAC NGG 2 0.1067 Tier II
21 NC_000002.12 2 217947421 - TNS1 NNNCTCTGTCTGCAGCTCAC NGG 2 0.0867 Tier II
22 NC_000005.10 5 1116731 - SLC12A7 NNNCTGTGTCTGCAACTCAC NGG 2 0.0667 Tier II
23 NC_000017.11 17 42041174 + ZNF385C NNNCTCTGTGTGCAAGACAC NGG 2 0.0598 Tier II
24 NC_000002.12 2 286594 - ALKAL2 NNNCTCTGTCTGCATCCCAC NGG 2 0.0353 Tier II
25 NC_000014.9 14 79829980 + NRXN3 NNNATCTGTCTGCAACACAC NTG 2 0.0328 Tier II
26 NC_000004.12 4 7529260 + SORCS2 NNNCTCTGTCTGCAACCCAG NGG 2 0.0104 Tier II
27 NC_000011.10 11 114313445 - NNMT NNNCTCTGTCTGGAACACAG NGG 2 0.008 Tier II
28 NC_000005.10 5 38436189 + EGFLAM NNNCTCTGTCTGCAACAGAC NTG 2 0.0052 Tier II
29 NC_000020.11 20 32886680 - EFCAB8 NNNCTCTGTCTGCAACACAC NCT 2 0.0 Tier II
30 NC_000004.12 4 154161249 + LOC101927947 NNNCTCTGTATGAAACACAC NGG 2 0.3333 Tier III
31 NC_000002.12 2 89168045 + IGK NNNCTCTGTCTCCAACACAA NGG 2 0.2647 Tier III
32 NC_000002.12 2 90013909 - IGK NNNCTCTGTCTCCAACACAA NGG 2 0.2647 Tier III
33 NC_000005.10 5 72637506 + TNPO1-DT NNNCTCTTTCTGGAACACAC NGG 2 0.0852 Tier III
34 NC_000002.12 2 286594 - LOC101927262 NNNCTCTGTCTGCATCCCAC NGG 2 0.0353 Tier III
35 NC_000010.11 10 79023124 + ZMIZ1-AS1 NNNCTCTGTCTGCAACCCAC NGC 2 0.0039 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 43488976 - Tpk1 NNNCTCTGTCTTCAACACAT NGG 2 0.1154 Tier I
2 NC_000075.6 9 105665025 - Pik3r4 NNNCTCTGCCTGTAACACAC NGG 2 0.6462 Tier II
3 NC_000068.7 2 130248164 - Tmc2 NNNCTTTCTCTGCAACACAC NGG 2 0.5714 Tier II
4 NC_000073.6 7 114028599 + Spon1 NNNCTCTGTATGCAGCACAC NGG 2 0.5633 Tier II
5 NC_000072.6 6 29574437 - Tnpo3 NNNCTCTGCCTGCTACACAC NGG 2 0.4923 Tier II
6 NC_000067.6 1 82469239 + Col4a4 NNNCTCAGTCTGCGACACAC NGG 2 0.4083 Tier II
7 NC_000072.6 6 112877934 + Srgap3 NNNCTCTGTCTTCAAAACAC NGG 2 0.3846 Tier II
8 NC_000080.6 14 28366781 - Erc2 NNNCTCTGTCAGCAACACAA NGG 2 0.375 Tier II
9 NC_000071.6 5 92672896 - Ccdc158 NNNCTCTGACTTCAACACAC NGG 2 0.3571 Tier II
10 NC_000073.6 7 138238008 + Tcerg1l NNNCTGGGTCTGCAACACAC NGG 2 0.2941 Tier II
11 NC_000080.6 14 64191377 + Msra NNNCTCTGTGTGTAACACAC NGG 2 0.2722 Tier II
12 NC_000072.6 6 17988788 + Wnt2 NNNCTCTGTCAGCAATACAC NGG 2 0.2308 Tier II
13 NC_000074.6 8 77258484 + Arhgap10 NNNCTCTGTCTTCAACAAAC NGG 2 0.2071 Tier II
14 NC_000076.6 10 63121295 + Mypn NNNCTCTGACTGCCACACAC NGG 2 0.199 Tier II
15 NC_000071.6 5 121883964 - Cux2 NNNCTTTGTCTGCCACACAC NGG 2 0.199 Tier II
16 NC_000077.6 11 57646411 + Galnt10 NNNGTCTGTCTGCACCACAC NGG 2 0.1364 Tier II
17 NC_000067.6 1 125818357 + Gpr39 NNNCTCTGTCTGCAACATCC NGG 2 0.133 Tier II
18 NC_000071.6 5 88567535 + Rufy3 NNNCTCTGCCTGGAACACAC NGG 2 0.1259 Tier II
19 NC_000071.6 5 136349636 + Cux1 NNNCTCTGTCTGAAACACAC NAG 2 0.0997 Tier II
20 NC_000074.6 8 123850199 + Ccsap NNNCTCTGTCTGCAGCTCAC NGG 2 0.0867 Tier II
21 NC_000080.6 14 122883171 - Pcca NNNGTCTGTCTGCAACAGAC NGG 2 0.0667 Tier II
22 NC_000085.6 19 24777346 + Pgm5 NNNCTCTGCCTGCAACACAC NGA 2 0.0641 Tier II
23 NC_000083.6 17 56288525 - Plin3 NNNCTCTGTCTTGAACACAC NGG 2 0.0524 Tier II
24 NC_000067.6 1 165790341 + Cd247 NNNCTCTGTCTGCACCTCAC NGG 2 0.0364 Tier II
25 NC_000074.6 8 121607126 + Zcchc14 NNNCTCTGTCTGCACCTCAC NGG 2 0.0364 Tier II
26 NC_000078.6 12 90300782 + Nrxn3 NNNATCTGTCTGCAACACAC NTG 2 0.0328 Tier II
27 NC_000074.6 8 120231533 - Gse1 NNNCTCTGTCTGCAAGACCC NGG 2 0.0318 Tier II
28 NC_000068.7 2 163061035 + Mybl2 NNNCTCTGTCTCCAACACAG NGG 2 0.0311 Tier II
29 NC_000078.6 12 5174176 + Klhl29 NNNCTCTGTCTGCAGCACAC NTG 2 0.0253 Tier II
30 NC_000068.7 2 35044989 - Hc NNNCTCTGTCTGAAACACAC NTG 2 0.015 Tier II
31 NC_000077.6 11 101043665 - Atp6v0a1 NNNCTCTGTCTGCATCACAC NGA 2 0.0139 Tier II
32 NC_000071.6 5 38387688 - Slc2a9 NNNTTCTGTCTGCAACACAC NGT 2 0.0129 Tier II
33 NC_000068.7 2 10591019 + Sfmbt2 NNNCTCTGTCTGCCACACAC NTG 2 0.0083 Tier II
34 NC_000072.6 6 23763071 - Cadps2 NNNCTCTGTCTGCAAGACAC NTG 2 0.006 Tier II
35 NC_000079.6 13 52743963 + BB123696 NNNATCTGTCTGCAAAACAC NGG 2 0.8421 Tier III
36 NC_000073.6 7 41629588 + Gm51480 NNNTTCTGTTTGCAACACAC NGG 2 0.7529 Tier III
37 NC_000079.6 13 7473374 - Gm36074 NNNATCTGTCTGCAGCACAC NGG 2 0.5474 Tier III
38 NC_000080.6 14 28366781 - Gm52097 NNNCTCTGTCAGCAACACAA NGG 2 0.375 Tier III
39 NC_000073.6 7 138238008 + Gm51503 NNNCTGGGTCTGCAACACAC NGG 2 0.2941 Tier III
40 NC_000071.6 5 121883964 - Gm52785 NNNCTTTGTCTGCCACACAC NGG 2 0.199 Tier III
41 NC_000077.6 11 57646411 + 4933424L21Rik NNNGTCTGTCTGCACCACAC NGG 2 0.1364 Tier III
42 NC_000067.6 1 163580092 + Gm32333 NNNCTCTGTCTGGATCACAC NGG 2 0.0273 Tier III
43 NC_000067.6 1 163580092 + Gm39689 NNNCTCTGTCTGGATCACAC NGG 2 0.0273 Tier III
44 NC_000073.6 7 70268544 - Gm29683 NNNCTCTTTCTGCAACACAC NTG 2 0.0244 Tier III
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Other clones with same target sequence:

(none)