Construct: sgRNA BRDN0001487063
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTCTCTGTCTGCAACACAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK17A (9263)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77915
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000072.6 |
6 |
43488976 |
- |
Tpk1 |
NNNCTCTGTCTTCAACACAT |
NGG |
2 |
0.1154 |
Tier I |
2 |
NC_000075.6 |
9 |
105665025 |
- |
Pik3r4 |
NNNCTCTGCCTGTAACACAC |
NGG |
2 |
0.6462 |
Tier II |
3 |
NC_000068.7 |
2 |
130248164 |
- |
Tmc2 |
NNNCTTTCTCTGCAACACAC |
NGG |
2 |
0.5714 |
Tier II |
4 |
NC_000073.6 |
7 |
114028599 |
+ |
Spon1 |
NNNCTCTGTATGCAGCACAC |
NGG |
2 |
0.5633 |
Tier II |
5 |
NC_000072.6 |
6 |
29574437 |
- |
Tnpo3 |
NNNCTCTGCCTGCTACACAC |
NGG |
2 |
0.4923 |
Tier II |
6 |
NC_000067.6 |
1 |
82469239 |
+ |
Col4a4 |
NNNCTCAGTCTGCGACACAC |
NGG |
2 |
0.4083 |
Tier II |
7 |
NC_000072.6 |
6 |
112877934 |
+ |
Srgap3 |
NNNCTCTGTCTTCAAAACAC |
NGG |
2 |
0.3846 |
Tier II |
8 |
NC_000080.6 |
14 |
28366781 |
- |
Erc2 |
NNNCTCTGTCAGCAACACAA |
NGG |
2 |
0.375 |
Tier II |
9 |
NC_000071.6 |
5 |
92672896 |
- |
Ccdc158 |
NNNCTCTGACTTCAACACAC |
NGG |
2 |
0.3571 |
Tier II |
10 |
NC_000073.6 |
7 |
138238008 |
+ |
Tcerg1l |
NNNCTGGGTCTGCAACACAC |
NGG |
2 |
0.2941 |
Tier II |
11 |
NC_000080.6 |
14 |
64191377 |
+ |
Msra |
NNNCTCTGTGTGTAACACAC |
NGG |
2 |
0.2722 |
Tier II |
12 |
NC_000072.6 |
6 |
17988788 |
+ |
Wnt2 |
NNNCTCTGTCAGCAATACAC |
NGG |
2 |
0.2308 |
Tier II |
13 |
NC_000074.6 |
8 |
77258484 |
+ |
Arhgap10 |
NNNCTCTGTCTTCAACAAAC |
NGG |
2 |
0.2071 |
Tier II |
14 |
NC_000076.6 |
10 |
63121295 |
+ |
Mypn |
NNNCTCTGACTGCCACACAC |
NGG |
2 |
0.199 |
Tier II |
15 |
NC_000071.6 |
5 |
121883964 |
- |
Cux2 |
NNNCTTTGTCTGCCACACAC |
NGG |
2 |
0.199 |
Tier II |
16 |
NC_000077.6 |
11 |
57646411 |
+ |
Galnt10 |
NNNGTCTGTCTGCACCACAC |
NGG |
2 |
0.1364 |
Tier II |
17 |
NC_000067.6 |
1 |
125818357 |
+ |
Gpr39 |
NNNCTCTGTCTGCAACATCC |
NGG |
2 |
0.133 |
Tier II |
18 |
NC_000071.6 |
5 |
88567535 |
+ |
Rufy3 |
NNNCTCTGCCTGGAACACAC |
NGG |
2 |
0.1259 |
Tier II |
19 |
NC_000071.6 |
5 |
136349636 |
+ |
Cux1 |
NNNCTCTGTCTGAAACACAC |
NAG |
2 |
0.0997 |
Tier II |
20 |
NC_000074.6 |
8 |
123850199 |
+ |
Ccsap |
NNNCTCTGTCTGCAGCTCAC |
NGG |
2 |
0.0867 |
Tier II |
21 |
NC_000080.6 |
14 |
122883171 |
- |
Pcca |
NNNGTCTGTCTGCAACAGAC |
NGG |
2 |
0.0667 |
Tier II |
22 |
NC_000085.6 |
19 |
24777346 |
+ |
Pgm5 |
NNNCTCTGCCTGCAACACAC |
NGA |
2 |
0.0641 |
Tier II |
23 |
NC_000083.6 |
17 |
56288525 |
- |
Plin3 |
NNNCTCTGTCTTGAACACAC |
NGG |
2 |
0.0524 |
Tier II |
24 |
NC_000067.6 |
1 |
165790341 |
+ |
Cd247 |
NNNCTCTGTCTGCACCTCAC |
NGG |
2 |
0.0364 |
Tier II |
25 |
NC_000074.6 |
8 |
121607126 |
+ |
Zcchc14 |
NNNCTCTGTCTGCACCTCAC |
NGG |
2 |
0.0364 |
Tier II |
26 |
NC_000078.6 |
12 |
90300782 |
+ |
Nrxn3 |
NNNATCTGTCTGCAACACAC |
NTG |
2 |
0.0328 |
Tier II |
27 |
NC_000074.6 |
8 |
120231533 |
- |
Gse1 |
NNNCTCTGTCTGCAAGACCC |
NGG |
2 |
0.0318 |
Tier II |
28 |
NC_000068.7 |
2 |
163061035 |
+ |
Mybl2 |
NNNCTCTGTCTCCAACACAG |
NGG |
2 |
0.0311 |
Tier II |
29 |
NC_000078.6 |
12 |
5174176 |
+ |
Klhl29 |
NNNCTCTGTCTGCAGCACAC |
NTG |
2 |
0.0253 |
Tier II |
30 |
NC_000068.7 |
2 |
35044989 |
- |
Hc |
NNNCTCTGTCTGAAACACAC |
NTG |
2 |
0.015 |
Tier II |
31 |
NC_000077.6 |
11 |
101043665 |
- |
Atp6v0a1 |
NNNCTCTGTCTGCATCACAC |
NGA |
2 |
0.0139 |
Tier II |
32 |
NC_000071.6 |
5 |
38387688 |
- |
Slc2a9 |
NNNTTCTGTCTGCAACACAC |
NGT |
2 |
0.0129 |
Tier II |
33 |
NC_000068.7 |
2 |
10591019 |
+ |
Sfmbt2 |
NNNCTCTGTCTGCCACACAC |
NTG |
2 |
0.0083 |
Tier II |
34 |
NC_000072.6 |
6 |
23763071 |
- |
Cadps2 |
NNNCTCTGTCTGCAAGACAC |
NTG |
2 |
0.006 |
Tier II |
35 |
NC_000079.6 |
13 |
52743963 |
+ |
BB123696 |
NNNATCTGTCTGCAAAACAC |
NGG |
2 |
0.8421 |
Tier III |
36 |
NC_000073.6 |
7 |
41629588 |
+ |
Gm51480 |
NNNTTCTGTTTGCAACACAC |
NGG |
2 |
0.7529 |
Tier III |
37 |
NC_000079.6 |
13 |
7473374 |
- |
Gm36074 |
NNNATCTGTCTGCAGCACAC |
NGG |
2 |
0.5474 |
Tier III |
38 |
NC_000080.6 |
14 |
28366781 |
- |
Gm52097 |
NNNCTCTGTCAGCAACACAA |
NGG |
2 |
0.375 |
Tier III |
39 |
NC_000073.6 |
7 |
138238008 |
+ |
Gm51503 |
NNNCTGGGTCTGCAACACAC |
NGG |
2 |
0.2941 |
Tier III |
40 |
NC_000071.6 |
5 |
121883964 |
- |
Gm52785 |
NNNCTTTGTCTGCCACACAC |
NGG |
2 |
0.199 |
Tier III |
41 |
NC_000077.6 |
11 |
57646411 |
+ |
4933424L21Rik |
NNNGTCTGTCTGCACCACAC |
NGG |
2 |
0.1364 |
Tier III |
42 |
NC_000067.6 |
1 |
163580092 |
+ |
Gm32333 |
NNNCTCTGTCTGGATCACAC |
NGG |
2 |
0.0273 |
Tier III |
43 |
NC_000067.6 |
1 |
163580092 |
+ |
Gm39689 |
NNNCTCTGTCTGGATCACAC |
NGG |
2 |
0.0273 |
Tier III |
44 |
NC_000073.6 |
7 |
70268544 |
- |
Gm29683 |
NNNCTCTTTCTGCAACACAC |
NTG |
2 |
0.0244 |
Tier III |
Other clones with same target sequence:
(none)