Construct: sgRNA BRDN0001487068

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CTTGTTGTAAATGTAACAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRD2 (6046)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77983

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 32975505 - BRD2 NNNGTTGTAAATGTAACAGT NGG 0 1.0 Tier I
2 NC_000009.12 9 129071920 + MIGA2 NNNGCTGTGAATGTAACAGT NGG 2 0.6667 Tier II
3 NC_000005.10 5 22604788 + CDH12 NNNGTTGCAAATGTAACAGA NGG 2 0.5625 Tier II
4 NC_000006.12 6 148436885 + SASH1 NNNGTAGTAAATGAAACAGT NGG 2 0.5365 Tier II
5 NC_000003.12 3 179385334 - MFN1 NNNGGTCTAAATGTAACAGT NGG 2 0.44 Tier II
6 NC_000002.12 2 113632143 + RABL2A NNNGTTGTAAAGGTAACATT NGG 2 0.3333 Tier II
7 NC_000022.11 22 50778754 - RABL2B NNNGTTGTAAAGGTAACATT NGG 2 0.3333 Tier II
8 NC_000005.10 5 98899207 - CHD1 NNNGTTGTAAATGTAAAATT NGG 2 0.3111 Tier II
9 NC_000003.12 3 130996302 + ATP2C1 NNNGTTGTAAATGTAACAGT NAG 1 0.2593 Tier II
10 NC_000017.11 17 8521577 - MYH10 NNNATTGTAAATGTAACAGT NAG 2 0.2333 Tier II
11 NC_000001.11 1 65345050 + DNAJC6 NNNGTTGTAAATGTAACAGA NAG 2 0.1458 Tier II
12 NC_000003.12 3 173860556 + NLGN1 NNNGGTGTAAATGTTACAGT NGG 2 0.128 Tier II
13 NC_000006.12 6 148880607 - UST NNNGTTGTAAATGTAACATG NGG 2 0.1176 Tier II
14 NC_000002.12 2 31371495 - XDH NNNGTTGTAACTGCAACAGT NGG 2 0.1143 Tier II
15 NC_000007.14 7 116997816 - ST7 NNNTTTGTAAATGTAACAGT NAG 2 0.0943 Tier II
16 NC_000022.11 22 40437824 - MRTFA NNNGTTGTAAATGTAAACGT NGG 2 0.0889 Tier II
17 NC_000010.11 10 47985636 - ANXA8 NNNGTTGTAAATCTTACAGT NGG 2 0.0842 Tier II
18 NC_000013.11 13 98910325 - DOCK9 NNNATTGTAAATGTAACAGT NTG 2 0.0351 Tier II
19 NC_000009.12 9 71367473 + TRPM3 NNNTTTGTAAATGTAACAGT NTG 2 0.0142 Tier II
20 NC_000013.11 13 112827058 - ATP11A NNNGTTGTAAATGTAACAGC NCG 2 0.0097 Tier II
21 NC_000005.10 5 122997008 + SNX24 NNNGTTGTAAATGGAACAGT NGT 2 0.0 Tier II
22 NC_000009.12 9 91752419 - ROR2 NNNGTTGTACATGTATCAGT NGG 2 0.0 Tier II
23 NC_000003.12 3 164148386 + LOC102724419 NNNGTGGGAAATGTAACAGT NGG 2 0.419 Tier III
24 NC_000022.11 22 50778754 - RPL23AP82 NNNGTTGTAAAGGTAACATT NGG 2 0.3333 Tier III
25 NC_000010.11 10 46399017 + LINC00842 NNNGTTGTAAATCTTACAGT NGG 2 0.0842 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 34116215 + Brd2 NNNGTTATAAATGTAACAGT NGG 1 1.0 Tier I
2 NC_000072.6 6 61759061 + Ccser1 NNNATTGTAAATGTAACAGT NGG 1 0.9 Tier II
3 NC_000071.6 5 35087801 - Dok7 NNNGTTAAAAATGTAACAGT NGG 2 0.8 Tier II
4 NC_000071.6 5 128837499 - Rimbp2 NNNGGTGTAAATGTAACAGT NGG 1 0.64 Tier II
5 NC_000069.6 3 154675082 + Erich3 NNNATTGTAAGTGTAACAGT NGG 2 0.585 Tier II
6 NC_000078.6 12 98862003 - Eml5 NNNGTTGTAGATGTAACAGT NGG 1 0.5556 Tier II
7 NC_000069.6 3 121774546 - Abcd3 NNNGTTTTATATGTAACAGT NGG 2 0.5042 Tier II
8 NC_000080.6 14 124041951 + Fgf14 NNNGTTATAAATGTAACTGT NGG 2 0.5 Tier II
9 NC_000082.6 16 41747049 + Lsamp NNNGTTGAAAATGTAACAGA NGG 2 0.45 Tier II
10 NC_000075.6 9 28439451 + Opcml NNNGTTGTAAATGTAACACT NGG 1 0.4483 Tier II
11 NC_000068.7 2 155164598 + Itch NNNGTTGTAGATGTAACAAT NGG 2 0.3968 Tier II
12 NC_000086.7 X 147421704 + Il13ra2 NNNGGTGTAGATGTAACAGT NGG 2 0.3556 Tier II
13 NC_000084.6 18 59062858 - Minar2 NNNTTTGAAAATGTAACAGT NGG 2 0.2909 Tier II
14 NC_000070.6 4 45307349 + Trmt10b NNNCTTGTAAAGGTAACAGT NGG 2 0.2647 Tier II
15 NC_000071.6 5 130551879 + Caln1 NNNGATGTAAAGGTAACAGT NGG 2 0.25 Tier II
16 NC_000074.6 8 65739548 - Marchf1 NNNGTTGTAAATGTAACATT NAG 2 0.1728 Tier II
17 NC_000068.7 2 68954406 - Cers6 NNNGTTGTAATTCTAACAGT NGG 2 0.1296 Tier II
18 NC_000085.6 19 22260318 - Trpm3 NNNGTTGTAAATGTAACCTT NGG 2 0.127 Tier II
19 NC_000071.6 5 138088859 + Zkscan1 NNNGTTGTAGATGTAGCAGT NGG 2 0.1068 Tier II
20 NC_000076.6 10 88351246 + Dram1 NNNGTTGTAAATTTAAGAGT NGG 2 0.0176 Tier II
21 NC_000067.6 1 162210787 - Dnm3 NNNGTTGTAAATGTAACAGT NGT 1 0.0161 Tier II
22 NC_000069.6 3 145368748 + Col24a1 NNNGCTGTAAATGTATCAGT NGG 2 0.0 Tier II
23 NC_000074.6 8 24952220 - Adam9 NNNGTTGTAAATGAATCAGT NGG 2 0.0 Tier II
24 NC_000070.6 4 121179301 - Rlf NNNGTTGTAAATGGATCAGT NGG 2 0.0 Tier II
25 NC_000076.6 10 31572793 - Rnf217 NNNGTTGTAAATGTATCATT NGG 2 0.0 Tier II
26 NC_000067.6 1 61846479 + Pard3b NNNGTTGTAAATTTATCAGT NGG 2 0.0 Tier II
27 NC_000072.6 6 54182975 - Chn2 NNNGTTGTAATTGTATCAGT NGG 2 0.0 Tier II
28 NC_000070.6 4 77945065 - Ptprd NNNGTTGTACATGTATCAGT NGG 2 0.0 Tier II
29 NC_000085.6 19 17459173 - Pcsk5 NNNGTTGTAGATGTACCAGT NGG 2 0.0 Tier II
30 NC_000069.6 3 100614545 - Man1a2 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
31 NC_000071.6 5 102787277 + Arhgap24 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
32 NC_000074.6 8 17438975 - C030002A05Rik NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
33 NC_000074.6 8 17438975 - Csmd1 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
34 NC_000074.6 8 65724154 - Marchf1 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
35 NC_000074.6 8 85832229 - Itfg1 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
36 NC_000075.6 9 64879313 + Dennd4a NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
37 NC_000076.6 10 20482760 - Pde7b NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
38 NC_000076.6 10 76109557 - Slc5a4b NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
39 NC_000077.6 11 58284392 + 4930438A08Rik NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
40 NC_000081.6 15 72940219 - Trappc9 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
41 NC_000084.6 18 89392821 + Dok6 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
42 NC_000085.6 19 50412339 + Sorcs1 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
43 NC_000086.7 X 42964128 + Tenm1 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier II
44 NC_000069.6 3 101023556 - Cd101 NNNGTTGTGAATGTATCAGT NGG 2 0.0 Tier II
45 NC_000079.6 13 23933483 - Gm33516 NNNGCTGGAAATGTAACAGT NGG 2 0.7333 Tier III
46 NC_000067.6 1 41778027 + Gm29260 NNNGTTGTAGATGTGACAGT NGG 2 0.3611 Tier III
47 NC_000071.6 5 86375441 - Gm35367 NNNGTTGTAAATGAAACAGA NGG 2 0.3482 Tier III
48 NC_000076.6 10 27784445 + Gm30228 NNNGTTGTAAATGTAACCGT NGG 1 0.1905 Tier III
49 NC_000085.6 19 22260318 - Gm33755 NNNGTTGTAAATGTAACCTT NGG 2 0.127 Tier III
50 NC_000067.6 1 96615759 + 4930533P14Rik NNNGTTGTAAATGTAGCTGT NGG 2 0.0962 Tier III
51 NC_000072.6 6 134341212 - 4933406J09Rik NNNGCTGTAAATGTATCAGT NGG 2 0.0 Tier III
52 NC_000080.6 14 46665578 - D330046F09Rik NNNGTTGTAAAAGTACCAGT NGG 2 0.0 Tier III
53 NC_000086.7 X 153047211 - 9530051G07Rik NNNGTTGTAAATATATCAGT NGG 2 0.0 Tier III
54 NC_000072.6 6 92978188 - 9530026P05Rik NNNGTTGTAAATGTATCAAT NGG 2 0.0 Tier III
55 NC_000068.7 2 144777239 - Gm39951 NNNGTTGTAAATGTATCAGT NGC 2 0.0 Tier III
56 NC_000080.6 14 53711352 - Tcra NNNGTTGTAAATGTATCAGT NTG 2 0.0 Tier III
57 NC_000067.6 1 61846479 + Pard3bos1 NNNGTTGTAAATTTATCAGT NGG 2 0.0 Tier III
58 NC_000067.6 1 79789828 - Gm39666 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier III
59 NC_000077.6 11 58284392 + Gm39827 NNNGTTGTAGATGTATCAGT NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)