Construct: sgRNA BRDN0001487069

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTATAATGGATGTAAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IPMK (253430)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78074

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 58216253 - IPMK NNNTAATGGATGTAAAGATA NGG 0 1.0 Tier I
2 NC_000002.12 2 97702718 + C2orf92 NNNTAATGGATGGAAAGACA NGG 2 0.072 Tier I
3 NC_000007.14 7 150183643 - ACTR3C NNNTATTAGATGTAAAGATA NGG 2 0.7143 Tier II
4 NC_000007.14 7 153783612 + DPP6 NNNTATTAGATGTAAAGATA NGG 2 0.7143 Tier II
5 NC_000006.12 6 41080098 + NFYA NNNTAGTGGATGTAAAAATA NGG 2 0.6667 Tier II
6 NC_000006.12 6 41080098 + OARD1 NNNTAGTGGATGTAAAAATA NGG 2 0.6667 Tier II
7 NC_000010.11 10 115864348 + ATRNL1 NNNTAATGGATGTAGAAATA NGG 2 0.6067 Tier II
8 NC_000002.12 2 202785334 - ICA1L NNNTAATGGATGTAAAATTA NGG 2 0.4667 Tier II
9 NC_000004.12 4 90860658 + CCSER1 NNNTAATGGATTTAAAAATA NGG 2 0.359 Tier II
10 NC_000016.10 16 70275329 + AARS1 NNNGAATGGATCTAAAGATA NGG 2 0.3309 Tier II
11 NC_000004.12 4 94467940 - PDLIM5 NNNTAAGGGATGTGAAGATA NGG 2 0.2745 Tier II
12 NC_000006.12 6 130068107 - L3MBTL3 NNNTAATGGATATAAAGAAA NGG 2 0.2667 Tier II
13 NC_000002.12 2 181985032 - PPP1R1C NNNAAATGTATGTAAAGATA NGG 2 0.254 Tier II
14 NC_000011.10 11 34310220 - ABTB2 NNNTAATGGATCTGAAGATA NGG 2 0.2471 Tier II
15 NC_000006.12 6 8039337 + BLOC1S5 NNNTAATGGATATAAATATA NGG 2 0.2333 Tier II
16 NC_000012.12 12 96799030 - CFAP54 NNNTAATGGATATAAATATA NGG 2 0.2333 Tier II
17 NC_000003.12 3 154425325 + GPR149 NNNTAAAGGATGTAAAGAGA NGG 2 0.2188 Tier II
18 NC_000009.12 9 116635181 - ASTN2 NNNTGATGGATGTAAAGAAA NGG 2 0.2057 Tier II
19 NC_000009.12 9 23749388 + ELAVL2 NNNTAATGGATGTCAAAATA NGG 2 0.2 Tier II
20 NC_000012.12 12 70623929 + PTPRB NNNTAATGGATGTAAAGAGG NGG 2 0.1912 Tier II
21 NC_000004.12 4 38024285 + TBC1D1 NNNTAATGGTTGTCAAGATA NGG 2 0.1891 Tier II
22 NC_000009.12 9 104921937 + ABCA1 NNNTAATGGAAGTAAAGAGA NGG 2 0.1875 Tier II
23 NC_000023.11 X 132659944 - HS6ST2 NNNTAATGCATGTAAAGAAA NGG 2 0.1538 Tier II
24 NC_000012.12 12 16275238 + SLC15A5 NNNTAAGGGATGGAAAGATA NGG 2 0.1535 Tier II
25 NC_000009.12 9 110333113 + TXNDC8 NNNTAATGGGTGTAAAGATA NAG 2 0.144 Tier II
26 NC_000009.12 9 35324006 - UNC13B NNNTAATGCATGTAAAGATA NAG 2 0.1396 Tier II
27 NC_000003.12 3 177081016 + TBL1XR1 NNNTAAAGGATGTAAAGATA NGA 2 0.0608 Tier II
28 NC_000012.12 12 2838084 - ITFG2 NNNTAATGGATGTCAAGACA NGG 2 0.0591 Tier II
29 NC_000001.11 1 198707144 - PTPRC NNNTAATGGATGTAAACAGA NGG 2 0.0588 Tier II
30 NC_000005.10 5 59237374 - PDE4D NNNTAATGGATGTAAAGAAA NTG 2 0.0111 Tier II
31 NC_000011.10 11 59359963 - OR5AN1 NNNTAATTGATGTAAAGATA NGT 2 0.0101 Tier II
32 NC_000021.9 21 37723733 + KCNJ6 NNNTAATGGATGTAAAGATA NCA 2 0.0 Tier II
33 NC_000002.12 2 28938530 - WDR43 NNNTAATGGATGTAATGATG NGG 2 0.0 Tier II
34 NC_000007.14 7 21023007 - LINC01162 NNNTAATGGATATAAAAATA NGG 2 0.8711 Tier III
35 NC_000013.11 13 22837821 - IPMKP1 NNNTAATGAATATAAAGATA NGG 2 0.6 Tier III
36 NC_000007.14 7 45202192 - LOC124901623 NNNTAATAGATTTAAAGATA NGG 2 0.3846 Tier III
37 NC_000010.11 10 119019383 - LINC03036 NNNTAATAGATGTAAAGAAA NGG 2 0.2857 Tier III
38 NC_000019.10 19 15911387 - LOC729654 NNNTAATGGATGTAAAGACA NGG 1 0.2759 Tier III
39 NC_000001.11 1 100702351 + LOC124904231 NNNAAATGGATGTAAAGTTA NGG 2 0.2381 Tier III
40 NC_000006.12 6 8039337 + BLOC1S5-TXNDC5 NNNTAATGGATATAAATATA NGG 2 0.2333 Tier III
41 NC_000006.12 6 8039337 + EEF1E1-BLOC1S5 NNNTAATGGATATAAATATA NGG 2 0.2333 Tier III
42 NC_000009.12 9 116635181 - LOC105376240 NNNTGATGGATGTAAAGAAA NGG 2 0.2057 Tier III
43 NC_000009.12 9 104921937 + LOC124902239 NNNTAATGGAAGTAAAGAGA NGG 2 0.1875 Tier III
44 NC_000012.12 12 16275238 + LOC101928362 NNNTAAGGGATGGAAAGATA NGG 2 0.1535 Tier III
45 NC_000004.12 4 123768617 + LINC01091 NNNTAATGGATGTTAAGAAA NGG 2 0.1524 Tier III
46 NC_000007.14 7 118357995 - LOC124901815 NNNTAATGGATGTAAAAATA NCG 2 0.1 Tier III
47 NC_000003.12 3 180547036 - TTC14-DT NNNTAATGGATGTAAAGGTA NGA 2 0.0278 Tier III
48 NC_000021.9 21 37723733 + KCNJ6-AS1 NNNTAATGGATGTAAAGATA NCA 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 161996620 + Nhs NNNCAATGGATATAAAGATA NGG 2 0.6039 Tier II
2 NC_000077.6 11 47225313 + Sgcd NNNTAGTGGATGTAAAGATG NGG 2 0.5462 Tier II
3 NC_000081.6 15 58051828 + Zhx1 NNNTAGTGGAAGTAAAGATA NGG 2 0.5357 Tier II
4 NC_000068.7 2 20441544 + Etl4 NNNTAATGGATATTAAGATA NGG 2 0.4978 Tier II
5 NC_000069.6 3 156947261 - Negr1 NNNAAATGGTTGTAAAGATA NGG 2 0.4202 Tier II
6 NC_000082.6 16 44255531 + Sidt1 NNNTAATGGAAGTTAAGATA NGG 2 0.4 Tier II
7 NC_000068.7 2 7108585 - Celf2 NNNTCATGGATCTAAAGATA NGG 2 0.2647 Tier II
8 NC_000071.6 5 72344058 + Corin NNNCAATGGATTTAAAGATA NGG 2 0.2489 Tier II
9 NC_000076.6 10 116549697 + Cnot2 NNNTAAAGGATGTAAATATA NGG 2 0.2188 Tier II
10 NC_000073.6 7 120997037 - Mfsd13b NNNTAATGGATGAACAGATA NGG 2 0.1888 Tier II
11 NC_000082.6 16 39997726 - Lsamp NNNTAATGAATGTAAAGAAA NGG 2 0.1837 Tier II
12 NC_000083.6 17 26114080 + Pgap6 NNNGAATGGATGTAAAGAAA NGG 2 0.1786 Tier II
13 NC_000070.6 4 125683956 + Grik3 NNNTAATGGATGAATAGATA NGG 2 0.1385 Tier II
14 NC_000078.6 12 117733383 - Rapgef5 NNNAAATGGATGTAAAGACA NGG 2 0.1314 Tier II
15 NC_000067.6 1 180574371 + Parp1 NNNTAACGGATGTAAAGCTA NGG 2 0.131 Tier II
16 NC_000075.6 9 86415811 - Ube2cbp NNNTAATGGCTGTAAAGAAA NGG 2 0.0952 Tier II
17 NC_000074.6 8 36710027 + Dlc1 NNNTAATGGATGGAAAGAAA NGG 2 0.0745 Tier II
18 NC_000068.7 2 57340224 - Gpd2 NNNTAATGGATGTCAAGACA NGG 2 0.0591 Tier II
19 NC_000081.6 15 57735978 + Zhx2 NNNTAATGGATGTAAGGATA NAG 2 0.0499 Tier II
20 NC_000075.6 9 118099934 - Cmc1 NNNTAATGGATGTAAAGTTA NGC 2 0.0111 Tier II
21 NC_000073.6 7 7555599 + Vmn2r33 NNNTAATGGATGTACAGATA NTG 2 0.0106 Tier II
22 NC_000078.6 12 30865662 - Gm32320 NNNTAAAGGATGCAAAGATA NGG 2 0.6908 Tier III
23 NC_000084.6 18 26083987 + Gm33228 NNNTAATGGATGCAAAGAAA NGG 2 0.2256 Tier III
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Other clones with same target sequence:

(none)