Construct: sgRNA BRDN0001487070
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGAGCTTGCCATTCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DCK (1633)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78058
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
71022494 |
- |
DCK |
NNNGAGCTTGCCATTCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
92012438 |
+ |
TRIP11 |
NNNGAGCTTACAATTCAGAG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000001.11 |
1 |
53026022 |
- |
SCP2 |
NNNGAGCTAGCCATTCAAAG |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000011.10 |
11 |
100761943 |
+ |
ARHGAP42 |
NNNGAGCTTACGATTCAGAG |
NGG |
2 |
0.4148 |
Tier II |
| 5 |
NC_000006.12 |
6 |
161764264 |
- |
PRKN |
NNNGAGCTTGCAATTCAGTG |
NGG |
2 |
0.3846 |
Tier II |
| 6 |
NC_000004.12 |
4 |
26198363 |
- |
RBPJ |
NNNGAGCTTACCATTTAGAG |
NGG |
2 |
0.2872 |
Tier II |
| 7 |
NC_000016.10 |
16 |
81602219 |
+ |
CMIP |
NNNGAGCTTGCCATTCAGAG |
NAG |
1 |
0.2593 |
Tier II |
| 8 |
NC_000001.11 |
1 |
21512630 |
- |
ALPL |
NNNGAGCTTGACACTCAGAG |
NGG |
2 |
0.2143 |
Tier II |
| 9 |
NC_000004.12 |
4 |
121360839 |
- |
QRFPR |
NNNGAGTTTGGCATTCAGAG |
NGG |
2 |
0.2031 |
Tier II |
| 10 |
NC_000001.11 |
1 |
14583535 |
+ |
KAZN |
NNNGAGCTGGCCTTTCAGAG |
NGG |
2 |
0.1857 |
Tier II |
| 11 |
NC_000001.11 |
1 |
3341386 |
+ |
PRDM16 |
NNNGAGCTTCCCATCCAGAG |
NGG |
2 |
0.1091 |
Tier II |
| 12 |
NC_000002.12 |
2 |
196544971 |
- |
HECW2 |
NNNGAGCTTGCCCTTGAGAG |
NGG |
2 |
0.0324 |
Tier II |
| 13 |
NC_000015.10 |
15 |
80535830 |
+ |
ARNT2 |
NNNGAGCTTTCCATTCAGAG |
NTG |
2 |
0.0317 |
Tier II |
| 14 |
NC_000015.10 |
15 |
41311908 |
- |
OIP5 |
NNNGAGCTTGCCATTGAGAG |
NGA |
2 |
0.0107 |
Tier II |
| 15 |
NC_000017.11 |
17 |
1227001 |
+ |
ABR |
NNNGAGCTTGCCCTGCAGAG |
NGG |
2 |
0.0105 |
Tier II |
| 16 |
NC_000002.12 |
2 |
239137663 |
- |
HDAC4 |
NNNGAGCTTGCCCGTCAGAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000012.12 |
12 |
85291883 |
+ |
ALX1 |
NNNGAGCTTTCCAGTCAGAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000004.12 |
4 |
16254371 |
- |
TAPT1-AS1 |
NNNAAGCTTTCCATTCAGAG |
NGG |
2 |
0.7313 |
Tier III |
| 19 |
NC_000011.10 |
11 |
57146421 |
- |
LOC105369309 |
NNNGAGCATCCCATTCAGAG |
NGG |
2 |
0.32 |
Tier III |
| 20 |
NC_000004.12 |
4 |
26198363 |
- |
LOC124900690 |
NNNGAGCTTACCATTTAGAG |
NGG |
2 |
0.2872 |
Tier III |
| 21 |
NC_000001.11 |
1 |
200527013 |
- |
LOC107985459 |
NNNGAGCTTGGCTTTCAGAG |
NGG |
2 |
0.075 |
Tier III |
| 22 |
NC_000015.10 |
15 |
41311908 |
- |
OIP5-AS1 |
NNNGAGCTTGCCATTGAGAG |
NGA |
2 |
0.0107 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
88772757 |
- |
Dck |
NNNGAGCTTGCCATTGAGAG |
NGG |
1 |
0.1538 |
Tier I |
| 2 |
NC_000080.6 |
14 |
9917844 |
+ |
Fhit |
NNNGAGCTTACCATTCAGAT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000075.6 |
9 |
28194172 |
+ |
Opcml |
NNNGATCTTGACATTCAGAG |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000073.6 |
7 |
113250482 |
+ |
Arntl |
NNNGAGGCTGCCATTCAGAG |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000072.6 |
6 |
84854493 |
+ |
Exoc6b |
NNNGGGCTTGCCAATCAGAG |
NGG |
2 |
0.4457 |
Tier II |
| 6 |
NC_000081.6 |
15 |
83787968 |
+ |
Mpped1 |
NNNCAGCTTTCCATTCAGAG |
NGG |
2 |
0.4301 |
Tier II |
| 7 |
NC_000067.6 |
1 |
99796359 |
+ |
Cntnap5b |
NNNGAGCTTGCTATTCAGAC |
NGG |
2 |
0.2308 |
Tier II |
| 8 |
NC_000077.6 |
11 |
6164063 |
+ |
Nudcd3 |
NNNGTGCTTGCCATACAGAG |
NGG |
2 |
0.2105 |
Tier II |
| 9 |
NC_000080.6 |
14 |
65424787 |
- |
Pnoc |
NNNGGGCTTGCCATTCAGAG |
NAG |
2 |
0.1867 |
Tier II |
| 10 |
NC_000073.6 |
7 |
64785248 |
- |
Fam189a1 |
NNNGAGCTGGCCTTTCAGAG |
NGG |
2 |
0.1857 |
Tier II |
| 11 |
NC_000078.6 |
12 |
99216614 |
+ |
Foxn3 |
NNNTAGCTTGCCACTCAGAG |
NGG |
2 |
0.1039 |
Tier II |
| 12 |
NC_000072.6 |
6 |
54540180 |
- |
Scrn1 |
NNNGAGCTTGCCCTTCACAG |
NGG |
2 |
0.1003 |
Tier II |
| 13 |
NC_000071.6 |
5 |
74623747 |
- |
Lnx1 |
NNNTAGCTTGGCATTCAGAG |
NGG |
2 |
0.0909 |
Tier II |
| 14 |
NC_000085.6 |
19 |
58928768 |
+ |
Hspa12a |
NNNGAGCTTGTCATTCAGAG |
NAG |
2 |
0.0798 |
Tier II |
| 15 |
NC_000074.6 |
8 |
118884454 |
+ |
Cdh13 |
NNNGATCTTGCCATTCAGAG |
NCG |
2 |
0.0714 |
Tier II |
| 16 |
NC_000071.6 |
5 |
31692030 |
- |
Rbks |
NNNGAGCTTGGCATTCAGAG |
NAG |
2 |
0.0648 |
Tier II |
| 17 |
NC_000077.6 |
11 |
16852064 |
- |
Egfr |
NNNGAGCTTGCCATTCTCAG |
NGG |
2 |
0.0635 |
Tier II |
| 18 |
NC_000073.6 |
7 |
56155728 |
- |
Herc2 |
NNNGAGCCTGCCATTCAGAG |
NTG |
2 |
0.039 |
Tier II |
| 19 |
NC_000071.6 |
5 |
111228062 |
+ |
Ttc28 |
NNNGAGCTGGCCATGCAGAG |
NGG |
2 |
0.031 |
Tier II |
| 20 |
NC_000075.6 |
9 |
119009209 |
- |
Ctdspl |
NNNGAGCTTGCCAAGCAGAG |
NGG |
2 |
0.031 |
Tier II |
| 21 |
NC_000068.7 |
2 |
165318564 |
- |
Elmo2 |
NNNGAACTTGCCATTCAGAG |
NGC |
2 |
0.0222 |
Tier II |
| 22 |
NC_000084.6 |
18 |
39238142 |
- |
Arhgap26 |
NNNGTGCTTGCCATTCAGAG |
NGT |
2 |
0.0059 |
Tier II |
| 23 |
NC_000070.6 |
4 |
58306612 |
+ |
Musk |
NNNGAGCTTGCCACTCAGAG |
NGT |
2 |
0.0046 |
Tier II |
| 24 |
NC_000067.6 |
1 |
167077868 |
- |
Fam78b |
NNNGAGCTTGCCATTCTGAG |
NGC |
2 |
0.003 |
Tier II |
| 25 |
NC_000084.6 |
18 |
73668811 |
+ |
Smad4 |
NNNGAACTTGCCAGTCAGAG |
NGG |
2 |
0.0 |
Tier II |
| 26 |
NC_000073.6 |
7 |
113250482 |
+ |
Gm33721 |
NNNGAGGCTGCCATTCAGAG |
NGG |
2 |
0.4706 |
Tier III |
| 27 |
NC_000073.6 |
7 |
110741190 |
- |
Gm39068 |
NNNGAGCTTGACATCCAGAG |
NGG |
2 |
0.2045 |
Tier III |
| 28 |
NC_000086.7 |
X |
108447787 |
+ |
Gm9218 |
NNNGAGCTTGCCATTCAGCC |
NGG |
2 |
0.0887 |
Tier III |
| 29 |
NC_000072.6 |
6 |
59942686 |
- |
Gm18012 |
NNNGAGCTTGCCATTGACAG |
NGG |
2 |
0.0733 |
Tier III |
Other clones with same target sequence:
(none)
