Construct: sgRNA BRDN0001487070

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTTGAGCTTGCCATTCAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCK (1633)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78058

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000004.12 4 71022494 - DCK NNNGAGCTTGCCATTCAGAG NGG 0 1.0 Tier I
2 NC_000014.9 14 92012438 + TRIP11 NNNGAGCTTACAATTCAGAG NGG 2 0.6667 Tier II
3 NC_000001.11 1 53026022 - SCP2 NNNGAGCTAGCCATTCAAAG NGG 2 0.6429 Tier II
4 NC_000011.10 11 100761943 + ARHGAP42 NNNGAGCTTACGATTCAGAG NGG 2 0.4148 Tier II
5 NC_000006.12 6 161764264 - PRKN NNNGAGCTTGCAATTCAGTG NGG 2 0.3846 Tier II
6 NC_000004.12 4 26198363 - RBPJ NNNGAGCTTACCATTTAGAG NGG 2 0.2872 Tier II
7 NC_000016.10 16 81602219 + CMIP NNNGAGCTTGCCATTCAGAG NAG 1 0.2593 Tier II
8 NC_000001.11 1 21512630 - ALPL NNNGAGCTTGACACTCAGAG NGG 2 0.2143 Tier II
9 NC_000004.12 4 121360839 - QRFPR NNNGAGTTTGGCATTCAGAG NGG 2 0.2031 Tier II
10 NC_000001.11 1 14583535 + KAZN NNNGAGCTGGCCTTTCAGAG NGG 2 0.1857 Tier II
11 NC_000001.11 1 3341386 + PRDM16 NNNGAGCTTCCCATCCAGAG NGG 2 0.1091 Tier II
12 NC_000002.12 2 196544971 - HECW2 NNNGAGCTTGCCCTTGAGAG NGG 2 0.0324 Tier II
13 NC_000015.10 15 80535830 + ARNT2 NNNGAGCTTTCCATTCAGAG NTG 2 0.0317 Tier II
14 NC_000015.10 15 41311908 - OIP5 NNNGAGCTTGCCATTGAGAG NGA 2 0.0107 Tier II
15 NC_000017.11 17 1227001 + ABR NNNGAGCTTGCCCTGCAGAG NGG 2 0.0105 Tier II
16 NC_000002.12 2 239137663 - HDAC4 NNNGAGCTTGCCCGTCAGAG NGG 2 0.0 Tier II
17 NC_000012.12 12 85291883 + ALX1 NNNGAGCTTTCCAGTCAGAG NGG 2 0.0 Tier II
18 NC_000004.12 4 16254371 - TAPT1-AS1 NNNAAGCTTTCCATTCAGAG NGG 2 0.7313 Tier III
19 NC_000011.10 11 57146421 - LOC105369309 NNNGAGCATCCCATTCAGAG NGG 2 0.32 Tier III
20 NC_000004.12 4 26198363 - LOC124900690 NNNGAGCTTACCATTTAGAG NGG 2 0.2872 Tier III
21 NC_000001.11 1 200527013 - LOC107985459 NNNGAGCTTGGCTTTCAGAG NGG 2 0.075 Tier III
22 NC_000015.10 15 41311908 - OIP5-AS1 NNNGAGCTTGCCATTGAGAG NGA 2 0.0107 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 88772757 - Dck NNNGAGCTTGCCATTGAGAG NGG 1 0.1538 Tier I
2 NC_000080.6 14 9917844 + Fhit NNNGAGCTTACCATTCAGAT NGG 2 0.6533 Tier II
3 NC_000075.6 9 28194172 + Opcml NNNGATCTTGACATTCAGAG NGG 2 0.5 Tier II
4 NC_000073.6 7 113250482 + Arntl NNNGAGGCTGCCATTCAGAG NGG 2 0.4706 Tier II
5 NC_000072.6 6 84854493 + Exoc6b NNNGGGCTTGCCAATCAGAG NGG 2 0.4457 Tier II
6 NC_000081.6 15 83787968 + Mpped1 NNNCAGCTTTCCATTCAGAG NGG 2 0.4301 Tier II
7 NC_000067.6 1 99796359 + Cntnap5b NNNGAGCTTGCTATTCAGAC NGG 2 0.2308 Tier II
8 NC_000077.6 11 6164063 + Nudcd3 NNNGTGCTTGCCATACAGAG NGG 2 0.2105 Tier II
9 NC_000080.6 14 65424787 - Pnoc NNNGGGCTTGCCATTCAGAG NAG 2 0.1867 Tier II
10 NC_000073.6 7 64785248 - Fam189a1 NNNGAGCTGGCCTTTCAGAG NGG 2 0.1857 Tier II
11 NC_000078.6 12 99216614 + Foxn3 NNNTAGCTTGCCACTCAGAG NGG 2 0.1039 Tier II
12 NC_000072.6 6 54540180 - Scrn1 NNNGAGCTTGCCCTTCACAG NGG 2 0.1003 Tier II
13 NC_000071.6 5 74623747 - Lnx1 NNNTAGCTTGGCATTCAGAG NGG 2 0.0909 Tier II
14 NC_000085.6 19 58928768 + Hspa12a NNNGAGCTTGTCATTCAGAG NAG 2 0.0798 Tier II
15 NC_000074.6 8 118884454 + Cdh13 NNNGATCTTGCCATTCAGAG NCG 2 0.0714 Tier II
16 NC_000071.6 5 31692030 - Rbks NNNGAGCTTGGCATTCAGAG NAG 2 0.0648 Tier II
17 NC_000077.6 11 16852064 - Egfr NNNGAGCTTGCCATTCTCAG NGG 2 0.0635 Tier II
18 NC_000073.6 7 56155728 - Herc2 NNNGAGCCTGCCATTCAGAG NTG 2 0.039 Tier II
19 NC_000071.6 5 111228062 + Ttc28 NNNGAGCTGGCCATGCAGAG NGG 2 0.031 Tier II
20 NC_000075.6 9 119009209 - Ctdspl NNNGAGCTTGCCAAGCAGAG NGG 2 0.031 Tier II
21 NC_000068.7 2 165318564 - Elmo2 NNNGAACTTGCCATTCAGAG NGC 2 0.0222 Tier II
22 NC_000084.6 18 39238142 - Arhgap26 NNNGTGCTTGCCATTCAGAG NGT 2 0.0059 Tier II
23 NC_000070.6 4 58306612 + Musk NNNGAGCTTGCCACTCAGAG NGT 2 0.0046 Tier II
24 NC_000067.6 1 167077868 - Fam78b NNNGAGCTTGCCATTCTGAG NGC 2 0.003 Tier II
25 NC_000084.6 18 73668811 + Smad4 NNNGAACTTGCCAGTCAGAG NGG 2 0.0 Tier II
26 NC_000073.6 7 113250482 + Gm33721 NNNGAGGCTGCCATTCAGAG NGG 2 0.4706 Tier III
27 NC_000073.6 7 110741190 - Gm39068 NNNGAGCTTGACATCCAGAG NGG 2 0.2045 Tier III
28 NC_000086.7 X 108447787 + Gm9218 NNNGAGCTTGCCATTCAGCC NGG 2 0.0887 Tier III
29 NC_000072.6 6 59942686 - Gm18012 NNNGAGCTTGCCATTGACAG NGG 2 0.0733 Tier III
Download CSV

Other clones with same target sequence:

(none)

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