Construct: sgRNA BRDN0001487080
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTTAGGCATTACCCACACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1A (8394)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77962
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000069.6 |
3 |
95074075 |
- |
Pip5k1a |
NNNAGGCATCACTCACACTG |
NGG |
2 |
0.3733 |
Tier I |
2 |
NC_000071.6 |
5 |
127984425 |
+ |
Tmem132d |
NNNAGGCATCAGCCACACTG |
NGG |
2 |
0.237 |
Tier I |
3 |
NC_000073.6 |
7 |
27532326 |
+ |
Pld3 |
NNNAGGCATTGCCCACACTG |
NTG |
2 |
0.0253 |
Tier I |
4 |
NC_000075.6 |
9 |
15913176 |
- |
Fat3 |
NNNAGGCATTATCCACACTG |
NGG |
1 |
0.5385 |
Tier II |
5 |
NC_000075.6 |
9 |
119796767 |
+ |
Scn11a |
NNNAGGCATCACCCACACTG |
NGG |
1 |
0.5333 |
Tier II |
6 |
NC_000073.6 |
7 |
120747183 |
- |
Vwa3a |
NNNAGGCAAGACCCACACTG |
NGG |
2 |
0.4643 |
Tier II |
7 |
NC_000075.6 |
9 |
114095060 |
- |
Susd5 |
NNNAGTCATCACCCACACTG |
NGG |
2 |
0.3556 |
Tier II |
8 |
NC_000077.6 |
11 |
53123243 |
- |
Fstl4 |
NNNAGCCATTACCCACACTC |
NGG |
2 |
0.2922 |
Tier II |
9 |
NC_000072.6 |
6 |
121219257 |
- |
Tuba8 |
NNNAGGCATTGCCCACACTC |
NGG |
2 |
0.2786 |
Tier II |
10 |
NC_000072.6 |
6 |
46397929 |
+ |
Cntnap2 |
NNNATGCAATACCCACACTG |
NGG |
2 |
0.2786 |
Tier II |
11 |
NC_000067.6 |
1 |
97416568 |
+ |
Gm7135 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier II |
12 |
NC_000068.7 |
2 |
127078381 |
- |
Blvra |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier II |
13 |
NC_000084.6 |
18 |
10733383 |
+ |
Mib1 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier II |
14 |
NC_000084.6 |
18 |
11883021 |
+ |
Cables1 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier II |
15 |
NC_000077.6 |
11 |
108725104 |
+ |
Cep112 |
NNNAGGCATTTCCCACATTG |
NGG |
2 |
0.1978 |
Tier II |
16 |
NC_000074.6 |
8 |
13373423 |
+ |
Tfdp1 |
NNNAGGCATTGCCCACACTG |
NAG |
2 |
0.1685 |
Tier II |
17 |
NC_000083.6 |
17 |
48974808 |
+ |
Lrfn2 |
NNNAGGCATTTGCCACACTG |
NGG |
2 |
0.1368 |
Tier II |
18 |
NC_000077.6 |
11 |
88407044 |
- |
Msi2 |
NNNAGGCATTACCTACTCTG |
NGG |
2 |
0.0978 |
Tier II |
19 |
NC_000078.6 |
12 |
88907600 |
- |
Nrxn3 |
NNNAGGCATTGCCCACTCTG |
NGG |
2 |
0.0867 |
Tier II |
20 |
NC_000067.6 |
1 |
88238797 |
- |
Mroh2a |
NNNAGGCTTTACCCACAGTG |
NGG |
2 |
0.0571 |
Tier II |
21 |
NC_000082.6 |
16 |
18928208 |
+ |
Hira |
NNNATGCATTACGCACACTG |
NGG |
2 |
0.0409 |
Tier II |
22 |
NC_000068.7 |
2 |
119679458 |
+ |
Rtf1 |
NNNAGGCATTACACACACTG |
NGA |
2 |
0.0267 |
Tier II |
23 |
NC_000081.6 |
15 |
5455621 |
- |
Gm46496 |
NNNAGGCATAACCCACACTG |
NGG |
1 |
0.8571 |
Tier III |
24 |
NC_000077.6 |
11 |
39591049 |
+ |
Gm33963 |
NNNAGGTATCACCCACACTG |
NGG |
2 |
0.4333 |
Tier III |
25 |
NC_000070.6 |
4 |
89055307 |
+ |
Wincr1 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier III |
26 |
NC_000078.6 |
12 |
97351813 |
+ |
Gm40552 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier III |
27 |
NC_000079.6 |
13 |
16203813 |
+ |
Gm31711 |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier III |
28 |
NC_000086.7 |
X |
20971747 |
+ |
A230072C01Rik |
NNNTGGCATTCCCCACACTG |
NGG |
2 |
0.2545 |
Tier III |
29 |
NC_000077.6 |
11 |
108725104 |
+ |
LOC115487814 |
NNNAGGCATTTCCCACATTG |
NGG |
2 |
0.1978 |
Tier III |
30 |
NC_000081.6 |
15 |
38148337 |
- |
Gm35081 |
NNNAGGCATTCCCCATACTG |
NGG |
2 |
0.1231 |
Tier III |
31 |
NC_000079.6 |
13 |
56235998 |
+ |
4930550C17Rik |
NNNAGGCATTACCCCCAGTG |
NGG |
2 |
0.0364 |
Tier III |
32 |
NC_000081.6 |
15 |
99018848 |
+ |
Gm41398 |
NNNAGGCATTAGCCACACTG |
NGC |
2 |
0.0099 |
Tier III |
33 |
NC_000076.6 |
10 |
82185354 |
- |
Gm40720 |
NNNAGGCATTACCCACAATG |
NGT |
2 |
0.0087 |
Tier III |
Other clones with same target sequence:
(none)