Construct: sgRNA BRDN0001487080

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTTAGGCATTACCCACACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5K1A (8394)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77962

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 151231720 + PIP5K1A NNNAGGCATTACCCACACTG NGG 0 1.0 Tier I
2 NC_000002.12 2 218430864 + VIL1 NNNAGGCAAAACCCACACTG NGG 2 0.7959 Tier I
3 NC_000020.11 20 54160124 + CYP24A1 NNNAGGCACTACCTACACTG NGG 2 0.6769 Tier II
4 NC_000001.11 1 17638045 - ARHGEF10L NNNAGGCACTGCCCACACTG NGG 2 0.6 Tier II
5 NC_000008.11 8 140583035 - AGO2 NNNTGGCACTACCCACACTG NGG 2 0.5874 Tier II
6 NC_000008.11 8 140080369 - TRAPPC9 NNNACGCATTACCCACACTC NGG 2 0.3367 Tier II
7 NC_000002.12 2 121233895 + TFCP2L1 NNNAGGCAGCACCCACACTG NGG 2 0.3302 Tier II
8 NC_000002.12 2 102143412 + IL1R1 NNNAGGCCTTAACCACACTG NGG 2 0.3061 Tier II
9 NC_000010.11 10 127100893 + DOCK1 NNNAGGCCTTGCCCACACTG NGG 2 0.2786 Tier II
10 NC_000001.11 1 48789579 + AGBL4 NNNAGGCATGAGCCACACTG NGG 2 0.2222 Tier II
11 NC_000016.10 16 67004769 + CES4A NNNAGGCCTGACCCACACTG NGG 2 0.2143 Tier II
12 NC_000001.11 1 43811458 - ST3GAL3 NNNGGGCATTACCCATACTG NGG 2 0.1923 Tier II
13 NC_000015.10 15 31098750 + TRPM1 NNNAGGCATTACCCACCCTG NGG 1 0.1765 Tier II
14 NC_000017.11 17 41623618 + KRT17 NNNAGGCCTTCCCCACACTG NGG 2 0.1714 Tier II
15 NC_000022.11 22 46725834 - CERK NNNAGGCATTACCCAGACTG NGG 1 0.1538 Tier II
16 NC_000022.11 22 50403496 - PPP6R2 NNNAGGCAGTACCCACCCTG NGG 2 0.1092 Tier II
17 NC_000019.10 19 16384111 - EPS15L1 NNNAGGCATTATCCACGCTG NGG 2 0.095 Tier II
18 NC_000001.11 1 176881382 - ASTN1 NNNAGCCATTACCCACACTG NGA 2 0.0473 Tier II
19 NC_000023.11 X 2788956 - XG NNNAGGCATTAGCCACACTG NGC 2 0.0099 Tier II
20 NC_000016.10 16 66438952 - BEAN1 NNNAGGCATTACCCACAGTG NGC 2 0.003 Tier II
21 NC_000003.12 3 45869657 - LZTFL1 NNNAGGCATTACCCACACTG NTT 2 0.0 Tier II
22 NC_000010.11 10 93961270 - PIPSL NNNAGGCATTACCCACACTG NGG 0 1.0 Tier III
23 NC_000006.12 6 7986784 + PIP5K1P1 NNNAGGCATTACTTACACTG NGG 2 0.5133 Tier III
24 NC_000006.12 6 7986784 + BLOC1S5-TXNDC5 NNNAGGCATTACTTACACTG NGG 2 0.5133 Tier III
25 NC_000004.12 4 158361868 + LOC105377510 NNNAGGCATAACACACACTG NGG 2 0.3297 Tier III
26 NC_000012.12 12 11216746 + LOC107987435 NNNTGGCATTACCCACAGTG NGG 2 0.0848 Tier III
27 NC_000002.12 2 23007891 - LOC124908057 NNNAGGCATTACCCATACTG NGT 2 0.005 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 95074075 - Pip5k1a NNNAGGCATCACTCACACTG NGG 2 0.3733 Tier I
2 NC_000071.6 5 127984425 + Tmem132d NNNAGGCATCAGCCACACTG NGG 2 0.237 Tier I
3 NC_000073.6 7 27532326 + Pld3 NNNAGGCATTGCCCACACTG NTG 2 0.0253 Tier I
4 NC_000075.6 9 15913176 - Fat3 NNNAGGCATTATCCACACTG NGG 1 0.5385 Tier II
5 NC_000075.6 9 119796767 + Scn11a NNNAGGCATCACCCACACTG NGG 1 0.5333 Tier II
6 NC_000073.6 7 120747183 - Vwa3a NNNAGGCAAGACCCACACTG NGG 2 0.4643 Tier II
7 NC_000075.6 9 114095060 - Susd5 NNNAGTCATCACCCACACTG NGG 2 0.3556 Tier II
8 NC_000077.6 11 53123243 - Fstl4 NNNAGCCATTACCCACACTC NGG 2 0.2922 Tier II
9 NC_000072.6 6 121219257 - Tuba8 NNNAGGCATTGCCCACACTC NGG 2 0.2786 Tier II
10 NC_000072.6 6 46397929 + Cntnap2 NNNATGCAATACCCACACTG NGG 2 0.2786 Tier II
11 NC_000067.6 1 97416568 + Gm7135 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier II
12 NC_000068.7 2 127078381 - Blvra NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier II
13 NC_000084.6 18 10733383 + Mib1 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier II
14 NC_000084.6 18 11883021 + Cables1 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier II
15 NC_000077.6 11 108725104 + Cep112 NNNAGGCATTTCCCACATTG NGG 2 0.1978 Tier II
16 NC_000074.6 8 13373423 + Tfdp1 NNNAGGCATTGCCCACACTG NAG 2 0.1685 Tier II
17 NC_000083.6 17 48974808 + Lrfn2 NNNAGGCATTTGCCACACTG NGG 2 0.1368 Tier II
18 NC_000077.6 11 88407044 - Msi2 NNNAGGCATTACCTACTCTG NGG 2 0.0978 Tier II
19 NC_000078.6 12 88907600 - Nrxn3 NNNAGGCATTGCCCACTCTG NGG 2 0.0867 Tier II
20 NC_000067.6 1 88238797 - Mroh2a NNNAGGCTTTACCCACAGTG NGG 2 0.0571 Tier II
21 NC_000082.6 16 18928208 + Hira NNNATGCATTACGCACACTG NGG 2 0.0409 Tier II
22 NC_000068.7 2 119679458 + Rtf1 NNNAGGCATTACACACACTG NGA 2 0.0267 Tier II
23 NC_000081.6 15 5455621 - Gm46496 NNNAGGCATAACCCACACTG NGG 1 0.8571 Tier III
24 NC_000077.6 11 39591049 + Gm33963 NNNAGGTATCACCCACACTG NGG 2 0.4333 Tier III
25 NC_000070.6 4 89055307 + Wincr1 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier III
26 NC_000078.6 12 97351813 + Gm40552 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier III
27 NC_000079.6 13 16203813 + Gm31711 NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier III
28 NC_000086.7 X 20971747 + A230072C01Rik NNNTGGCATTCCCCACACTG NGG 2 0.2545 Tier III
29 NC_000077.6 11 108725104 + LOC115487814 NNNAGGCATTTCCCACATTG NGG 2 0.1978 Tier III
30 NC_000081.6 15 38148337 - Gm35081 NNNAGGCATTCCCCATACTG NGG 2 0.1231 Tier III
31 NC_000079.6 13 56235998 + 4930550C17Rik NNNAGGCATTACCCCCAGTG NGG 2 0.0364 Tier III
32 NC_000081.6 15 99018848 + Gm41398 NNNAGGCATTAGCCACACTG NGC 2 0.0099 Tier III
33 NC_000076.6 10 82185354 - Gm40720 NNNAGGCATTACCCACAATG NGT 2 0.0087 Tier III
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Other clones with same target sequence:

(none)