Construct: sgRNA BRDN0001487083

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAAACTGCAACCAACAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAPK8 (5599)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78047

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 48425965 + MAPK8 NNNAACTGCAACCAACAGTA NGG 0 1.0 Tier I
2 NC_000002.12 2 98616483 - UNC50 NNNAACTGCAACCAACCATA NGG 2 0.1222 Tier I
3 NC_000011.10 11 114712364 + NXPE2 NNNAAAGGCAACCAACAGTA NGG 2 0.5462 Tier II
4 NC_000012.12 12 29501895 - TMTC1 NNNAACTGCAATCAAAAGTA NGG 2 0.5385 Tier II
5 NC_000009.12 9 127356953 - GARNL3 NNNAAATGCAATCAACAGTA NGG 2 0.5 Tier II
6 NC_000013.11 13 43669171 + ENOX1 NNNAACTGCAACCAGCAATA NGG 2 0.45 Tier II
7 NC_000001.11 1 48569203 - AGBL4 NNNAACTGCAACCACAAGTA NGG 2 0.2727 Tier II
8 NC_000017.11 17 80676678 + RPTOR NNNAACTGCAACCAAAAGTA NAG 2 0.2593 Tier II
9 NC_000001.11 1 227260820 + CDC42BPA NNNATCTGGAACCAACAGTA NGG 2 0.2251 Tier II
10 NC_000002.12 2 52908671 + LOC105369165 NNNAACTACAACCAACAGTG NGG 2 0.7647 Tier III
11 NC_000017.11 17 48672739 + LINC02086 NNNAACAGCAACTAACAGTA NGG 2 0.6125 Tier III
12 NC_000005.10 5 66395299 - LOC105379003 NNNAACTGGAACCAACACTA NGG 2 0.2948 Tier III
13 NC_000004.12 4 185099994 + LINC02436 NNNAAGTGCAAGCAACAGTA NGG 2 0.2222 Tier III
14 NC_000023.11 X 23101148 - PTCHD1-AS NNNAACTGCAACAAACAGTA NGA 2 0.0267 Tier III
15 NC_000020.11 20 52405318 + LOC105372666 NNNAACTGCAACCCACAGTA NGA 2 0.0149 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 33388906 - Mapk8 NNNAACTACAACCAACAGTA NGG 1 1.0 Tier I
2 NC_000075.6 9 107979459 + Uba7 NNNAACTGCTACCAACAGTA NGG 1 0.8824 Tier I
3 NC_000086.7 X 166474239 + Rab9 NNNAACTAAAACCAACAGTA NGG 2 0.8571 Tier II
4 NC_000079.6 13 54106032 - Sfxn1 NNNAAATGTAACCAACAGTA NGG 2 0.8125 Tier II
5 NC_000069.6 3 86281942 - Lrba NNNAAATGCAACCAACAATA NGG 2 0.6429 Tier II
6 NC_000070.6 4 33246780 + Pnrc1 NNNAACTGTAAACAACAGTA NGG 2 0.625 Tier II
7 NC_000071.6 5 14806178 + Pclo NNNAACTGCAAACAACAGTG NGG 2 0.5462 Tier II
8 NC_000067.6 1 52984997 + 1700019D03Rik NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier II
9 NC_000073.6 7 56371739 + Oca2 NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier II
10 NC_000076.6 10 53055006 - Slc35f1 NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier II
11 NC_000078.6 12 116505631 - Ptprn2 NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier II
12 NC_000079.6 13 17781787 + Cdk13 NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier II
13 NC_000075.6 9 103330160 + Topbp1 NNNAACTGCAACCAACATTT NGG 2 0.4 Tier II
14 NC_000074.6 8 58071504 + Galntl6 NNNAACTGGAATCAACAGTA NGG 2 0.3333 Tier II
15 NC_000077.6 11 64063718 + Cox10 NNNAACAGCAACCAACAGAA NGG 2 0.25 Tier II
16 NC_000068.7 2 15759113 - Malrd1 NNNTACTGCATCCAACAGTA NGG 2 0.1958 Tier II
17 NC_000068.7 2 44716577 + Gtdc1 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
18 NC_000072.6 6 57621568 + Herc6 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
19 NC_000076.6 10 27567949 + Lama2 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
20 NC_000079.6 13 77740689 - Fam172a NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
21 NC_000079.6 13 77782999 - Fam172a NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
22 NC_000079.6 13 77787112 - Fam172a NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
23 NC_000085.6 19 40053005 + Cyp2c54 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
24 NC_000085.6 19 40783108 + Cc2d2b NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
25 NC_000086.7 X 95533975 - Asb12 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
26 NC_000086.7 X 113070429 + Chm NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier II
27 NC_000071.6 5 34996875 - Rgs12 NNNAACTTCAACCAACAGTA NAG 2 0.162 Tier II
28 NC_000080.6 14 51628652 + Gm5622 NNNAACTGCAACCTACAGAA NGG 2 0.1524 Tier II
29 NC_000071.6 5 25801137 - Actr3b NNNAACTGCAGCCAACAGTA NCG 2 0.0696 Tier II
30 NC_000073.6 7 17280201 + Gm42373 NNNAACAGCAACCAACAGTT NGG 2 0.525 Tier III
31 NC_000070.6 4 17512522 + Gm42257 NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier III
32 NC_000071.6 5 28633051 - 9530036O11Rik NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier III
33 NC_000072.6 6 69689930 + Igk NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier III
34 NC_000075.6 9 24982363 + E130101E03Rik NNNAACTGCAACCAACAGAT NGG 2 0.1714 Tier III
35 NC_000069.6 3 126485622 + Gm46823 NNNAACGGCAACCAACAGAA NGG 2 0.1681 Tier III
36 NC_000082.6 16 39463100 - Gm36903 NNNAACTGCAACCTACAGAA NGG 2 0.1524 Tier III
37 NC_000073.6 7 118279168 + Gm34999 NNNAACTGCAACCAACCCTA NGG 2 0.084 Tier III
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Other clones with same target sequence:

(none)