Construct: sgRNA BRDN0001487089
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGACCGACCATTCTTCCCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME2 (4831)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77932
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51168299 |
+ |
NME2 |
NNNACCGACCATTCTTCCCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51168299 |
+ |
NME1-NME2 |
NNNACCGACCATTCTTCCCT |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000003.12 |
3 |
14661700 |
- |
CCDC174 |
NNNACCAACCATTCTTCACT |
NGG |
2 |
0.5385 |
Tier I |
| 4 |
NC_000012.12 |
12 |
57808229 |
+ |
AVIL |
NNNATCGACCATTCTTTCCT |
NGG |
2 |
0.297 |
Tier I |
| 5 |
NC_000005.10 |
5 |
36660581 |
- |
SLC1A3 |
NNNACAGAACATTCTTCCCT |
NGG |
2 |
0.7959 |
Tier II |
| 6 |
NC_000003.12 |
3 |
185789385 |
+ |
IGF2BP2 |
NNNAGTGACCATTCTTCCCT |
NGG |
2 |
0.5571 |
Tier II |
| 7 |
NC_000001.11 |
1 |
58016240 |
+ |
DAB1 |
NNNACCAACCATTCTGCCCT |
NGG |
2 |
0.3462 |
Tier II |
| 8 |
NC_000007.14 |
7 |
129212530 |
+ |
SMO |
NNNACCGAGCATGCTTCCCT |
NGG |
2 |
0.1615 |
Tier II |
| 9 |
NC_000013.11 |
13 |
51259601 |
- |
FAM124A |
NNNACCGACCATTCTTCCCA |
NAG |
2 |
0.1458 |
Tier II |
| 10 |
NC_000006.12 |
6 |
166629195 |
+ |
RPS6KA2 |
NNNCCCGACCATGCTTCCCT |
NGG |
2 |
0.0921 |
Tier II |
| 11 |
NC_000008.11 |
8 |
60765951 |
- |
CHD7 |
NNNACTGACCATTCTTCCCT |
NGT |
2 |
0.015 |
Tier II |
| 12 |
NC_000005.10 |
5 |
164702381 |
- |
LINC03000 |
NNNACTGACCATACTTCCCT |
NGG |
2 |
0.6429 |
Tier III |
| 13 |
NC_000003.12 |
3 |
14661700 |
- |
LOC124906215 |
NNNACCAACCATTCTTCACT |
NGG |
2 |
0.5385 |
Tier III |
| 14 |
NC_000023.11 |
X |
10864025 |
+ |
HCCS-DT |
NNNACCAACCAGTCTTCCCT |
NGG |
2 |
0.5 |
Tier III |
| 15 |
NC_000013.11 |
13 |
30160312 |
+ |
LINC00385 |
NNNACCTACAATTCTTCCCT |
NGG |
2 |
0.4952 |
Tier III |
| 16 |
NC_000012.12 |
12 |
120282441 |
+ |
NME2P1 |
NNNACCGCCCATTCTTCCCT |
NGG |
1 |
0.4286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
6947701 |
+ |
Rbfox1 |
NNNACCCACCATTCTACCCT |
NGG |
2 |
0.625 |
Tier II |
| 2 |
NC_000071.6 |
5 |
116545907 |
+ |
Srrm4 |
NNNACCAACCATTCTTCCCA |
NGG |
2 |
0.5625 |
Tier II |
| 3 |
NC_000071.6 |
5 |
132007341 |
- |
Auts2 |
NNNTCCCACCATTCTTCCCT |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000067.6 |
1 |
40627282 |
+ |
Slc9a4 |
NNNAGCCACCATTCTTCCCT |
NGG |
2 |
0.4125 |
Tier II |
| 5 |
NC_000070.6 |
4 |
149360245 |
+ |
Ube4b |
NNNACAGACCCTTCTTCCCT |
NGG |
2 |
0.3714 |
Tier II |
| 6 |
NC_000067.6 |
1 |
8643521 |
- |
Sntg1 |
NNNACCGACCATTCTTCCAA |
NGG |
2 |
0.2411 |
Tier II |
| 7 |
NC_000086.7 |
X |
13658737 |
- |
Cask |
NNNACCAACCATTCTTCCCT |
NTG |
2 |
0.039 |
Tier II |
| 8 |
NC_000086.7 |
X |
13658737 |
- |
Gpr82 |
NNNACCAACCATTCTTCCCT |
NTG |
2 |
0.039 |
Tier II |
| 9 |
NC_000067.6 |
1 |
170300670 |
+ |
Gm7694 |
NNNACCTACCATTCTTCCCT |
NGC |
2 |
0.0127 |
Tier II |
| 10 |
NC_000078.6 |
12 |
29185139 |
- |
Gm31333 |
NNNACTGACCACTCTTCCCT |
NGG |
2 |
0.8797 |
Tier III |
| 11 |
NC_000077.6 |
11 |
95244036 |
- |
Gm11515 |
NNNACCCACCTTTCTTCCCT |
NGG |
2 |
0.2115 |
Tier III |
Other clones with same target sequence:
(none)
