Construct: sgRNA BRDN0001487091
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGAATCCCAAAAGTACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SH3BP4 (23677)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78089
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
235041335 |
+ |
SH3BP4 |
NNNGAATCCCAAAAGTACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
34842930 |
- |
BLTP3A |
NNNAAATCACAAAAGTACTG |
NGG |
2 |
0.7714 |
Tier II |
| 3 |
NC_000004.12 |
4 |
143459891 |
+ |
GAB1 |
NNNGAAACCAAAAAGTACTG |
NGG |
2 |
0.7583 |
Tier II |
| 4 |
NC_000017.11 |
17 |
17974299 |
- |
DRC3 |
NNNGAATTGCAAAAGTACTG |
NGG |
2 |
0.5417 |
Tier II |
| 5 |
NC_000007.14 |
7 |
103154233 |
+ |
LOC105375434 |
NNNGTATCCCAAAAGCACTG |
NGG |
2 |
0.2424 |
Tier II |
| 6 |
NC_000013.11 |
13 |
77974420 |
- |
EDNRB |
NNNGTATCCCAAAAGCACTG |
NGG |
2 |
0.2424 |
Tier II |
| 7 |
NC_000017.11 |
17 |
35502254 |
+ |
SLFN12L |
NNNGTATCCCAAAAGCACTG |
NGG |
2 |
0.2424 |
Tier II |
| 8 |
NC_000012.12 |
12 |
119866234 |
- |
CIT |
NNNGAATCCCAAGAGGACTG |
NGG |
2 |
0.2258 |
Tier II |
| 9 |
NC_000009.12 |
9 |
69215170 |
+ |
TJP2 |
NNNGAATTCCAAAAGTACGG |
NGG |
2 |
0.2188 |
Tier II |
| 10 |
NC_000020.11 |
20 |
42742134 |
+ |
PTPRT |
NNNGAATCCCAATAGCACTG |
NGG |
2 |
0.2 |
Tier II |
| 11 |
NC_000005.10 |
5 |
126961174 |
+ |
MARCHF3 |
NNNGAATCCCAGAAGTACTG |
NAG |
2 |
0.1872 |
Tier II |
| 12 |
NC_000001.11 |
1 |
63427730 |
+ |
ALG6 |
NNNGAATCCCAAAGGTACTG |
NAG |
2 |
0.121 |
Tier II |
| 13 |
NC_000007.14 |
7 |
11577209 |
- |
THSD7A |
NNNGAATCCCAGAAGTACTG |
NGA |
2 |
0.0502 |
Tier II |
| 14 |
NC_000002.12 |
2 |
36961385 |
+ |
STRN |
NNNCAATCCCAAAAGTACTG |
NGA |
2 |
0.0368 |
Tier II |
| 15 |
NC_000002.12 |
2 |
210299770 |
+ |
MYL1 |
NNNGAAACCCAAAAGTACTG |
NTG |
2 |
0.0341 |
Tier II |
| 16 |
NC_000022.11 |
22 |
30053744 |
+ |
HORMAD2-AS1 |
NNNGAATCTCAAAAGCACTG |
NGG |
2 |
0.5833 |
Tier III |
| 17 |
NC_000020.11 |
20 |
29080486 |
- |
FRG1DP |
NNNGAATCCGAAAAGAACTG |
NGG |
2 |
0.3535 |
Tier III |
| 18 |
NC_000006.12 |
6 |
1361098 |
+ |
LINC01394 |
NNNGCCTCCCAAAAGTACTG |
NGG |
2 |
0.2273 |
Tier III |
| 19 |
NC_000003.12 |
3 |
196151033 |
- |
LINC00885 |
NNNGACTCCCAAAAGTGCTG |
NGG |
2 |
0.0802 |
Tier III |
| 20 |
NC_000021.9 |
21 |
45251558 |
- |
LINC00334 |
NNNGAATCCCAAAAGGCCTG |
NGG |
2 |
0.0611 |
Tier III |
| 21 |
NC_000023.11 |
X |
135683218 |
- |
SAGE2P |
NNNGAATCCCTAAATTACTG |
NGG |
2 |
0.044 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
77616507 |
- |
Ptprd |
NNNGAATCATAAAAGTACTG |
NGG |
2 |
0.8067 |
Tier II |
| 2 |
NC_000067.6 |
1 |
62514375 |
- |
Pard3b |
NNNGAATCCTAGAAGTACTG |
NGG |
2 |
0.6797 |
Tier II |
| 3 |
NC_000068.7 |
2 |
130505463 |
+ |
Ptpra |
NNNGAATTCCAAAAGTACTT |
NGG |
2 |
0.6125 |
Tier II |
| 4 |
NC_000070.6 |
4 |
74159698 |
- |
Frmd3 |
NNNGAACCCCAAAAGTATTG |
NGG |
2 |
0.442 |
Tier II |
| 5 |
NC_000070.6 |
4 |
138732194 |
- |
Pla2g2c |
NNNGAACCCCAAAGGTACTG |
NGG |
2 |
0.3208 |
Tier II |
| 6 |
NC_000070.6 |
4 |
138732194 |
- |
Ubxn10 |
NNNGAACCCCAAAGGTACTG |
NGG |
2 |
0.3208 |
Tier II |
| 7 |
NC_000074.6 |
8 |
3584646 |
+ |
Rps23rg1 |
NNNTAGTCCCAAAAGTACTG |
NGG |
2 |
0.2597 |
Tier II |
| 8 |
NC_000075.6 |
9 |
28045788 |
+ |
Opcml |
NNNGTATCCCAAAAGTAATG |
NGG |
2 |
0.1958 |
Tier II |
| 9 |
NC_000069.6 |
3 |
108690275 |
+ |
Gpsm2 |
NNNGAAACCCAAACGTACTG |
NGG |
2 |
0.1875 |
Tier II |
| 10 |
NC_000071.6 |
5 |
89030687 |
- |
Slc4a4 |
NNNGAATCCCAAAAGTATTG |
NAG |
2 |
0.1667 |
Tier II |
| 11 |
NC_000068.7 |
2 |
19304215 |
- |
Armc3 |
NNNGAATCCCAAAAGGACAG |
NGG |
2 |
0.0989 |
Tier II |
| 12 |
NC_000083.6 |
17 |
43057179 |
- |
Tnfrsf21 |
NNNGAATCCCACAACTACTG |
NGG |
2 |
0.0718 |
Tier II |
| 13 |
NC_000086.7 |
X |
8276977 |
- |
Slc38a5 |
NNNGAATCCCTACAGTACTG |
NGG |
2 |
0.0648 |
Tier II |
| 14 |
NC_000068.7 |
2 |
15613865 |
+ |
Malrd1 |
NNNGAACCCCAAAAGTACTG |
NGA |
2 |
0.0477 |
Tier II |
| 15 |
NC_000068.7 |
2 |
52707279 |
+ |
Stam2 |
NNNGAAGCCCAAAAGTACTG |
NGA |
2 |
0.0408 |
Tier II |
| 16 |
NC_000086.7 |
X |
95163784 |
+ |
Arhgef9 |
NNNGAATCCCAAACTTACTG |
NGG |
2 |
0.0306 |
Tier II |
| 17 |
NC_000082.6 |
16 |
3885216 |
- |
Naa60 |
NNNGAATACCAAAAGTACTG |
NTG |
2 |
0.0253 |
Tier II |
| 18 |
NC_000082.6 |
16 |
35178644 |
+ |
Adcy5 |
NNNGAATCCCAAAAGGACTG |
NGC |
2 |
0.0077 |
Tier II |
| 19 |
NC_000067.6 |
1 |
62514375 |
- |
Gm34881 |
NNNGAATCCTAGAAGTACTG |
NGG |
2 |
0.6797 |
Tier III |
| 20 |
NC_000070.6 |
4 |
138732194 |
- |
LOC115489908 |
NNNGAACCCCAAAGGTACTG |
NGG |
2 |
0.3208 |
Tier III |
| 21 |
NC_000071.6 |
5 |
91278367 |
+ |
Gm19619 |
NNNGAATTCCAATAGTACTG |
NGG |
2 |
0.2625 |
Tier III |
Other clones with same target sequence:
(none)
