Construct: sgRNA BRDN0001487092
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAGCGTACGACTGTGTACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK7 (5754)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78022
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43141974 |
+ |
PTK7 |
NNNCGTACGACTGTGTACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
7252351 |
+ |
ELP5 |
NNNCGTACGACTGTGCGCCA |
NGG |
2 |
0.1176 |
Tier II |
| 3 |
NC_000023.11 |
X |
43971516 |
- |
NDP |
NNNCTTACGACTGTGTACCA |
NGC |
2 |
0.0067 |
Tier II |
| 4 |
NC_000023.11 |
X |
43971516 |
- |
NDP-AS1 |
NNNCTTACGACTGTGTACCA |
NGC |
2 |
0.0067 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
46576600 |
- |
Ptk7 |
NNNCGTACAACTGTGTACCA |
NGG |
1 |
0.6429 |
Tier I |
| 2 |
NC_000069.6 |
3 |
89259659 |
+ |
Dpm3 |
NNNCGTATGACTGTGTACCG |
NGG |
2 |
0.6691 |
Tier II |
| 3 |
NC_000067.6 |
1 |
66848923 |
- |
Acadl |
NNNCGTATGAATGTGTACCA |
NGG |
2 |
0.6563 |
Tier II |
| 4 |
NC_000071.6 |
5 |
118086440 |
+ |
Fbxw8 |
NNNCGTATGAGTGTGTACCA |
NGG |
2 |
0.2188 |
Tier II |
| 5 |
NC_000078.6 |
12 |
73321463 |
- |
Slc38a6 |
NNNCGTACGGCTGTGTACCA |
NGT |
2 |
0.009 |
Tier II |
Other clones with same target sequence:
(none)
