Construct: sgRNA BRDN0001487095
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCCTCAAAGACTTCCGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SIK1 (150094)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77893
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000021.9 |
21 |
6116969 |
- |
LOC102724428 |
NNNCTCAAAGACTTCCGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000021.9 |
21 |
43421293 |
+ |
SIK1 |
NNNCTCAAAGACTTCCGGGG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000007.14 |
7 |
72961223 |
- |
TRIM74 |
NNNCTCAAAGCATTCCGGGG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000007.14 |
7 |
75403868 |
+ |
TRIM73 |
NNNCTCAAAGCATTCCGGGG |
NGG |
2 |
0.2857 |
Tier II |
| 5 |
NC_000016.10 |
16 |
67455215 |
- |
ATP6V0D1 |
NNNCTCAAAGACATCTGGGG |
NGG |
2 |
0.213 |
Tier II |
| 6 |
NC_000006.12 |
6 |
45886740 |
+ |
CLIC5 |
NNNCTCAAAGGCTTCTGGGG |
NGG |
2 |
0.2 |
Tier II |
| 7 |
NC_000002.12 |
2 |
79934588 |
- |
CTNNA2 |
NNNCTCAAAGACTTCTGGGC |
NGG |
2 |
0.1319 |
Tier II |
| 8 |
NC_000016.10 |
16 |
19610503 |
- |
VPS35L |
NNNCTCAATGACTTGCGGGG |
NGG |
2 |
0.03 |
Tier II |
| 9 |
NC_000005.10 |
5 |
80964540 |
+ |
RASGRF2 |
NNNCTCAAAGACTTCTGGGG |
NGA |
2 |
0.0214 |
Tier II |
| 10 |
NC_000008.11 |
8 |
66027230 |
- |
DNAJC5B |
NNNCTCAAAGATTTCCGGGG |
NTG |
2 |
0.021 |
Tier II |
| 11 |
NC_000016.10 |
16 |
89643274 |
+ |
LOC124903758 |
NNNCTCAAAGACTGCCAGGG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
33049986 |
+ |
Iqcg |
NNNCTCTATGACTTCCGGGG |
NGG |
2 |
0.2625 |
Tier I |
| 2 |
NC_000080.6 |
14 |
33958501 |
- |
Rbp3 |
NNNTTCAAAGACTTCTGGGG |
NGG |
2 |
0.2462 |
Tier I |
| 3 |
NC_000077.6 |
11 |
88440312 |
+ |
Msi2 |
NNNATCAAAGACTTCAGGGG |
NGG |
2 |
0.8421 |
Tier II |
| 4 |
NC_000077.6 |
11 |
67684285 |
+ |
Gas7 |
NNNTTCAAAGACTTCAGGGG |
NGG |
2 |
0.8 |
Tier II |
| 5 |
NC_000077.6 |
11 |
67684285 |
+ |
Glp2r |
NNNTTCAAAGACTTCAGGGG |
NGG |
2 |
0.8 |
Tier II |
| 6 |
NC_000068.7 |
2 |
119229456 |
- |
Ppp1r14d |
NNNCTCAGAGACTTCCAGGG |
NGG |
2 |
0.6844 |
Tier II |
| 7 |
NC_000071.6 |
5 |
151057386 |
- |
Stard13 |
NNNCTCAAAGAGTTCCGTGG |
NGG |
2 |
0.2963 |
Tier II |
| 8 |
NC_000077.6 |
11 |
37111453 |
+ |
Tenm2 |
NNNCTAAAAGACTTCTGGGG |
NGG |
2 |
0.2857 |
Tier II |
| 9 |
NC_000068.7 |
2 |
23327352 |
+ |
Nxph2 |
NNNCTCAAAGACTTCCAGGG |
NAG |
2 |
0.242 |
Tier II |
| 10 |
NC_000081.6 |
15 |
97792874 |
+ |
Hdac7 |
NNNCTCGAAGACTTCTGGGG |
NGG |
2 |
0.2172 |
Tier II |
| 11 |
NC_000074.6 |
8 |
87570346 |
- |
4933402J07Rik |
NNNCACAAAGACTTCCCGGG |
NGG |
2 |
0.1176 |
Tier II |
| 12 |
NC_000083.6 |
17 |
29434575 |
- |
Gm36486 |
NNNCTCACAGCCTTCCGGGG |
NGG |
2 |
0.1714 |
Tier III |
| 13 |
NC_000078.6 |
12 |
115434004 |
- |
Igh |
NNNGTCAAAGACTTCCTGGG |
NGG |
2 |
0.125 |
Tier III |
| 14 |
NC_000077.6 |
11 |
114554391 |
+ |
Gm31724 |
NNNCTCAAAGCCTCCCGGGG |
NGG |
2 |
0.1143 |
Tier III |
Other clones with same target sequence:
(none)
