Construct: sgRNA BRDN0001487098

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCCTGACGGGCGAGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CKM (1158)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78031

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000019.10 19 45311896 - CKM NNNCTGACGGGCGAGTTCAA NGG 0 1.0 Tier I
2 NC_000011.10 11 70503325 + SHANK2 NNNCTGACAGGCGAGTTCCA NGG 2 0.133 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 19416744 + Ckm NNNCTGACGGGCGAGTTCAA NGG 0 1.0 Tier I
2 NC_000081.6 15 102406394 - Sp1 NNNCTGAGGGGCGAGCTCAA NGG 2 0.4286 Tier II
3 NC_000075.6 9 36794280 + Ei24 NNNCTGACGGTCTAGTTCAA NGG 2 0.1154 Tier II
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Other clones with same target sequence:

(none)