Construct: sgRNA BRDN0001487105
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGTTGCTTAACGAATATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR2A (92)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77930
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
147915306 |
+ |
ACVR2A |
NNNGTTGCTTAACGAATATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
138701532 |
- |
FGF13 |
NNNGTTGCTTAATAAATATG |
NGG |
2 |
0.525 |
Tier II |
| 3 |
NC_000001.11 |
1 |
50820170 |
+ |
FAF1 |
NNNGTTGCTTAATGAATATG |
NAG |
2 |
0.1815 |
Tier II |
| 4 |
NC_000002.12 |
2 |
8984271 |
+ |
MBOAT2 |
NNNGTTGCTTAATGAATATG |
NAG |
2 |
0.1815 |
Tier II |
| 5 |
NC_000019.10 |
19 |
51748351 |
+ |
FPR1 |
NNNGTTGCTTAATGAATATG |
NAG |
2 |
0.1815 |
Tier II |
| 6 |
NC_000002.12 |
2 |
38857654 |
- |
DHX57 |
NNNGTTGCTTAACCAATATG |
NGA |
2 |
0.0298 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
48890413 |
+ |
Acvr2a |
NNNGTTGCTCAATGAATATG |
NGG |
2 |
0.3733 |
Tier I |
| 2 |
NC_000068.7 |
2 |
110909788 |
- |
Ano3 |
NNNGTTGCATAACAAATATG |
NGG |
2 |
0.6964 |
Tier II |
| 3 |
NC_000079.6 |
13 |
19070350 |
- |
Amph |
NNNGTAGCTTAACTAATATG |
NGG |
2 |
0.2311 |
Tier II |
| 4 |
NC_000084.6 |
18 |
12408967 |
- |
Lama3 |
NNNGTTGCTTAAGGAACATG |
NGG |
2 |
0.0963 |
Tier II |
| 5 |
NC_000072.6 |
6 |
68735201 |
- |
Igk |
NNNGTTGCTCAACAAATATG |
NGG |
2 |
0.4 |
Tier III |
| 6 |
NC_000078.6 |
12 |
114097373 |
- |
Igh |
NNNGTTGCTTAATGAATATG |
NAG |
2 |
0.1815 |
Tier III |
Other clones with same target sequence:
(none)
