Construct: sgRNA BRDN0001487114
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTTCCGATCAGAACCAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PXK (54899)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78019
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
58390626 |
+ |
PXK |
NNNTCCGATCAGAACCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
91443921 |
- |
NFIL3 |
NNNTCCAAACAGAACCAAAG |
NGG |
2 |
0.9286 |
Tier II |
| 3 |
NC_000001.11 |
1 |
115054671 |
+ |
TSPAN2 |
NNNTCTGATCAGAGCCAAAG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000016.10 |
16 |
88678301 |
- |
SNAI3 |
NNNTCCAATCAGAACCAAAG |
NAG |
2 |
0.2593 |
Tier II |
| 5 |
NC_000016.10 |
16 |
88678301 |
- |
SNAI3-AS1 |
NNNTCCAATCAGAACCAAAG |
NAG |
2 |
0.2593 |
Tier III |
| 6 |
NC_000002.12 |
2 |
12203791 |
+ |
MIR3681HG |
NNNTCCCATCAGAACCACAG |
NGG |
2 |
0.131 |
Tier III |
| 7 |
NC_000015.10 |
15 |
22269021 |
- |
REREP3 |
NNNTCTGATCAGAAGCAAAG |
NGG |
2 |
0.0464 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
8136913 |
+ |
Pxk |
NNNTCCGATCAGAACCAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000079.6 |
13 |
15621960 |
- |
Gli3 |
NNNTCCCATCAGAACAAAAG |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000078.6 |
12 |
21285273 |
- |
Itgb1bp1 |
NNNACCGCTCAGAACCAAAG |
NGG |
2 |
0.2041 |
Tier II |
| 4 |
NC_000068.7 |
2 |
157170661 |
+ |
Rbl1 |
NNNTCCGATCAAAACCAAAG |
NGA |
2 |
0.0648 |
Tier II |
| 5 |
NC_000074.6 |
8 |
128297568 |
- |
A930035F09Rik |
NNNTCTGATCAGAAGCAAAG |
NGG |
2 |
0.0464 |
Tier III |
Other clones with same target sequence:
(none)
