Construct: sgRNA BRDN0001487120
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCAAAAGGCGTTACCCCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- YES1 (7525)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77966
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
756629 |
+ |
YES1 |
NNNAAAAGGCGTTACCCCTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
25647460 |
- |
GRK3 |
NNNAAAAGGTGTTACCCCTG |
NGG |
1 |
0.9412 |
Tier II |
| 3 |
NC_000008.11 |
8 |
19740761 |
+ |
CSGALNACT1 |
NNNAAAAGCAGTTACCCCTG |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000015.10 |
15 |
58613103 |
+ |
ADAM10 |
NNNAAAAGGCGTTGCCACTG |
NGG |
2 |
0.2178 |
Tier II |
| 5 |
NC_000008.11 |
8 |
10940547 |
- |
XKR6 |
NNNAAAAGGGGTTTCCCCTG |
NGG |
2 |
0.2074 |
Tier II |
| 6 |
NC_000022.11 |
22 |
25647460 |
- |
YES1P1 |
NNNAAAAGGTGTTACCCCTG |
NGG |
1 |
0.9412 |
Tier III |
| 7 |
NC_000002.12 |
2 |
3154058 |
- |
LOC107985840 |
NNNAAAAGCGGTTACCCCTG |
NGG |
2 |
0.2094 |
Tier III |
| 8 |
NC_000008.11 |
8 |
64336503 |
+ |
LINC01414 |
NNNAAAAGGGGTTACCCCTG |
NGA |
2 |
0.027 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
32640532 |
- |
Yes1 |
NNNAAAAGGAGTCACCCCTG |
NGG |
2 |
0.6842 |
Tier I |
| 2 |
NC_000076.6 |
10 |
7739901 |
- |
Katna1 |
NNNAAAAGACGTTGCCCCTG |
NGG |
2 |
0.3 |
Tier II |
| 3 |
NC_000067.6 |
1 |
38428810 |
- |
Aff3 |
NNNAGAAGGCTTTACCCCTG |
NGG |
2 |
0.2769 |
Tier II |
Other clones with same target sequence:
(none)
