Construct: sgRNA BRDN0001487133
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAGATTGGCACAGCAACGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- C17orf75 (64149)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78079
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
32341271 |
- |
C17orf75 |
NNNATTGGCACAGCAACGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
80689637 |
+ |
CDYL2 |
NNNATTGGAACAGCAACAAG |
NGG |
2 |
0.5934 |
Tier II |
| 3 |
NC_000003.12 |
3 |
27220158 |
- |
NEK10 |
NNNATTTGCACAGCAACAAG |
NGG |
2 |
0.3956 |
Tier II |
| 4 |
NC_000003.12 |
3 |
124972574 |
+ |
HEG1 |
NNNATTGCCACAGCAACCAG |
NGG |
2 |
0.293 |
Tier II |
| 5 |
NC_000008.11 |
8 |
74060664 |
- |
LY96 |
NNNATTTGCACAGCAAAGAG |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000011.10 |
11 |
133465025 |
- |
OPCML |
NNNATTGGCACAGCAACCAC |
NGG |
2 |
0.2041 |
Tier II |
| 7 |
NC_000023.11 |
X |
48578596 |
+ |
RBM3 |
NNNAATGGCACATCAACGAG |
NGG |
2 |
0.15 |
Tier II |
| 8 |
NC_000001.11 |
1 |
243924266 |
- |
LINC02774 |
NNNATTGGCAGAGCAACCAG |
NGG |
2 |
0.119 |
Tier III |
| 9 |
NC_000017.11 |
17 |
1713764 |
- |
MIR22HG |
NNNATTGGCCCAGCAAGGAG |
NGG |
2 |
0.0196 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
18521499 |
- |
Nhsl1 |
NNNAGTGGCACAGCAACTAG |
NGG |
2 |
0.4267 |
Tier II |
| 2 |
NC_000068.7 |
2 |
69986464 |
+ |
Ubr3 |
NNNCTTAGCACAGCAACGAG |
NGG |
2 |
0.3529 |
Tier II |
| 3 |
NC_000079.6 |
13 |
44448624 |
+ |
1700029N11Rik |
NNNATTGTCACGGCAACGAG |
NGG |
2 |
0.4514 |
Tier III |
| 4 |
NC_000070.6 |
4 |
94996560 |
+ |
Gm12694 |
NNNATTGTCACATCAACGAG |
NGG |
2 |
0.1875 |
Tier III |
| 5 |
NC_000068.7 |
2 |
72457769 |
+ |
Gm39847 |
NNNGTTGGCACAGCAGCGAG |
NGG |
2 |
0.1202 |
Tier III |
Other clones with same target sequence:
(none)
