Construct: sgRNA BRDN0001487135

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TTGTGGCACAGATCGCGGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROR2 (4920)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77941

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 91724694 - ROR2 NNNTGGCACAGATCGCGGCG NGG 0 1.0 Tier I
2 NC_000019.10 19 40605584 + LTBP4 NNNTGGCACAGAGCGCGCCG NGG 2 0.1242 Tier I
3 NC_000018.10 18 11892 - LINC02564 NNNTGGCAGAGACCGCGGCG NGG 2 0.4887 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 53111255 - Ror2 NNNTGGCGCAGATCGCTGCG NGG 2 0.1833 Tier I
2 NC_000073.6 7 144612961 - Ano1 NNNTGGCACAGATCAAGGCG NGG 2 0.9412 Tier II
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Other clones with same target sequence:

(none)