Construct: sgRNA BRDN0001487137

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAACTGGACAGGTTTCATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPR1A (657)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77958

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 86919178 + BMPR1A NNNCTGGACAGGTTTCATAG NGG 0 1.0 Tier I
2 NC_000019.10 19 17436675 + TMEM221 NNNCTGGACAGGTTGAATAG NGG 2 0.05 Tier I
3 NC_000004.12 4 41008007 + APBB2 NNNCTAGAAAGGTTTCATAG NGG 2 0.8571 Tier II
4 NC_000020.11 20 42316843 - PTPRT NNNCTGGACAGGCTTCATAG NGG 1 0.7895 Tier II
5 NC_000015.10 15 44049822 - FRMD5 NNNCTGGGAAGGTTTCATAG NGG 2 0.6286 Tier II
6 NC_000014.9 14 79625690 - NRXN3 NNNCTGGAGAGGTTTCATAG NGG 1 0.619 Tier II
7 NC_000008.11 8 99787215 + VPS13B NNNCTGGAAAGGTTTCAAAG NGG 2 0.5714 Tier II
8 NC_000010.11 10 35355087 - CCNY NNNCTGGACAACTTTCATAG NGG 2 0.5294 Tier II
9 NC_000007.14 7 117422680 + ASZ1 NNNCTGGAAAGGTTTCACAG NGG 2 0.3673 Tier II
10 NC_000004.12 4 24842750 - CCDC149 NNNCTGGACAGGCTTCATGG NGG 2 0.2961 Tier II
11 NC_000015.10 15 29056864 - APBA2 NNNCTGGTCAGGTTTCAAAG NGG 2 0.2857 Tier II
12 NC_000011.10 11 34236457 + ABTB2 NNNCTCGACAGGTTTCAGAG NGG 2 0.2273 Tier II
13 NC_000009.12 9 127184465 - RALGPS1 NNNCTGGCCAGGTTTCACAG NGG 2 0.1837 Tier II
14 NC_000014.9 14 56649086 + TMEM260 NNNCTGGACAGGGTTCAGAG NGG 2 0.087 Tier II
15 NC_000001.11 1 33778847 + CSMD2 NNNCTGGACAGGTTTCTTAC NGG 2 0.0571 Tier II
16 NC_000011.10 11 132710943 + OPCML NNNCTGGAGAGGTTTCATAG NGA 2 0.043 Tier II
17 NC_000007.14 7 3998596 + SDK1 NNNCTGGACAGGTTTCAGAG NTG 2 0.013 Tier II
18 NC_000012.12 12 62143509 - TAFA2 NNNCTGGACAGGTTTTATAG NTG 2 0.012 Tier II
19 NC_000009.12 9 127729124 + TTC16 NNNCTGGACAGGTTGCTTAG NGG 2 0.0067 Tier II
20 NC_000014.9 14 79625690 - NRXN3-AS3 NNNCTGGAGAGGTTTCATAG NGG 1 0.619 Tier III
21 NC_000007.14 7 64226515 + LOC105375319 NNNCTAGACAGGTTTCACAG NGG 2 0.4286 Tier III
22 NC_000005.10 5 121840951 - LOC105379149 NNNCTAGACAGGTTTCATAG NAG 2 0.2593 Tier III
23 NC_000002.12 2 198612148 - LOC105373831 NNNCTGGAGAGGTTTCAGAG NGG 2 0.2063 Tier III
24 NC_000009.12 9 127184465 - LOC105376278 NNNCTGGCCAGGTTTCACAG NGG 2 0.1837 Tier III
25 NC_000004.12 4 54336014 + LINC02283 NNNCTGGACAAGTTTCCTAG NGG 2 0.1765 Tier III
26 NC_000015.10 15 69669977 - PCAT29 NNNCAGGACAGGTTTCCTAG NGG 2 0.0882 Tier III
27 NC_000003.12 3 181347649 - SOX2-OT NNNGTGGACAGGTTTGATAG NGG 2 0.0769 Tier III
28 NC_000005.10 5 164540715 + LOC102546299 NNNCTGGACAGGTGTCCTAG NGG 2 0.0 Tier III
29 NC_000005.10 5 164540715 + LINC03000 NNNCTGGACAGGTGTCCTAG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 36331194 + Sorcs2 NNNCTGGACAGGCTTCATAA NGG 2 0.7401 Tier II
2 NC_000067.6 1 132711490 - Nfasc NNNCTGGACAGACTTCATAG NGG 2 0.7368 Tier II
3 NC_000067.6 1 32178877 - Khdrbs2 NNNCTGAACAGCTTTCATAG NGG 2 0.5294 Tier II
4 NC_000076.6 10 89319579 + Ano4 NNNCTGGACAATTTTCATAG NGG 2 0.3846 Tier II
5 NC_000072.6 6 29695723 + Tspan33 NNNCTGGAAAGGTTTCACAG NGG 2 0.3673 Tier II
6 NC_000081.6 15 88694017 - Brd1 NNNCTGCTCAGGTTTCATAG NGG 2 0.2946 Tier II
7 NC_000071.6 5 48984156 - Kcnip4 NNNCTGGCCAGGTTTCATGG NGG 2 0.1607 Tier II
8 NC_000086.7 X 6460703 + Shroom4 NNNCTGGAGAGGTTTCATAG NAG 2 0.1605 Tier II
9 NC_000085.6 19 53797959 + Rbm20 NNNCTGGACAGGTCTCACAG NGG 2 0.1224 Tier II
10 NC_000077.6 11 35289804 + Slit3 NNNCTGGACAGGTCTCAGAG NGG 2 0.0952 Tier II
11 NC_000072.6 6 129401218 - Clec1b NNNCTGGACAGGTTTCTAAG NGG 2 0.0889 Tier II
12 NC_000072.6 6 129401218 - Clec12b NNNCTGGACAGGTTTCTAAG NGG 2 0.0889 Tier II
13 NC_000080.6 14 48513700 + Tmem260 NNNCTGGACAGGGTTCAGAG NGG 2 0.087 Tier II
14 NC_000085.6 19 57928963 + Atrnl1 NNNCTGGACAGGTTTTATAG NAG 2 0.0798 Tier II
15 NC_000069.6 3 138763678 + Tspan5 NNNCTGGTCAGGTTTCTTAG NGG 2 0.0571 Tier II
16 NC_000085.6 19 41503606 + Lcor NNNCTGGACAGGTTTCATAA NTG 2 0.0365 Tier II
17 NC_000084.6 18 21272595 - Garem1 NNNCTGGAGAGGTTTCATAG NGC 2 0.0138 Tier II
18 NC_000071.6 5 26859480 - Dpp6 NNNCTGGACAGCTTTCATAG NGC 2 0.0118 Tier II
19 NC_000067.6 1 88086151 + Ugt1a10 NNNCTGGACAGGTTCCATAG NTG 2 0.0106 Tier II
20 NC_000067.6 1 88086151 + Ugt1a9 NNNCTGGACAGGTTCCATAG NTG 2 0.0106 Tier II
21 NC_000071.6 5 23054305 + Lhfpl3 NNNCTGGACAGCTTTCATAG NGT 2 0.0085 Tier II
22 NC_000086.7 X 6460703 + 5430428K19Rik NNNCTGGAGAGGTTTCATAG NAG 2 0.1605 Tier III
23 NC_000086.7 X 8002177 - Gm39488 NNNCTGGACAGGTTTTATGG NGG 2 0.1154 Tier III
24 NC_000077.6 11 35289804 + Gm46277 NNNCTGGACAGGTCTCAGAG NGG 2 0.0952 Tier III
Download CSV

Other clones with same target sequence:

(none)