Construct: sgRNA BRDN0001487137
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAACTGGACAGGTTTCATAG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- BMPR1A (657)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000010.11 | 10 | 86919178 | + | BMPR1A | NNNCTGGACAGGTTTCATAG | NGG | 0 | 1.0 | Tier I |
2 | NC_000019.10 | 19 | 17436675 | + | TMEM221 | NNNCTGGACAGGTTGAATAG | NGG | 2 | 0.05 | Tier I |
3 | NC_000004.12 | 4 | 41008007 | + | APBB2 | NNNCTAGAAAGGTTTCATAG | NGG | 2 | 0.8571 | Tier II |
4 | NC_000020.11 | 20 | 42316843 | - | PTPRT | NNNCTGGACAGGCTTCATAG | NGG | 1 | 0.7895 | Tier II |
5 | NC_000015.10 | 15 | 44049822 | - | FRMD5 | NNNCTGGGAAGGTTTCATAG | NGG | 2 | 0.6286 | Tier II |
6 | NC_000014.9 | 14 | 79625690 | - | NRXN3 | NNNCTGGAGAGGTTTCATAG | NGG | 1 | 0.619 | Tier II |
7 | NC_000008.11 | 8 | 99787215 | + | VPS13B | NNNCTGGAAAGGTTTCAAAG | NGG | 2 | 0.5714 | Tier II |
8 | NC_000010.11 | 10 | 35355087 | - | CCNY | NNNCTGGACAACTTTCATAG | NGG | 2 | 0.5294 | Tier II |
9 | NC_000007.14 | 7 | 117422680 | + | ASZ1 | NNNCTGGAAAGGTTTCACAG | NGG | 2 | 0.3673 | Tier II |
10 | NC_000004.12 | 4 | 24842750 | - | CCDC149 | NNNCTGGACAGGCTTCATGG | NGG | 2 | 0.2961 | Tier II |
11 | NC_000015.10 | 15 | 29056864 | - | APBA2 | NNNCTGGTCAGGTTTCAAAG | NGG | 2 | 0.2857 | Tier II |
12 | NC_000011.10 | 11 | 34236457 | + | ABTB2 | NNNCTCGACAGGTTTCAGAG | NGG | 2 | 0.2273 | Tier II |
13 | NC_000009.12 | 9 | 127184465 | - | RALGPS1 | NNNCTGGCCAGGTTTCACAG | NGG | 2 | 0.1837 | Tier II |
14 | NC_000014.9 | 14 | 56649086 | + | TMEM260 | NNNCTGGACAGGGTTCAGAG | NGG | 2 | 0.087 | Tier II |
15 | NC_000001.11 | 1 | 33778847 | + | CSMD2 | NNNCTGGACAGGTTTCTTAC | NGG | 2 | 0.0571 | Tier II |
16 | NC_000011.10 | 11 | 132710943 | + | OPCML | NNNCTGGAGAGGTTTCATAG | NGA | 2 | 0.043 | Tier II |
17 | NC_000007.14 | 7 | 3998596 | + | SDK1 | NNNCTGGACAGGTTTCAGAG | NTG | 2 | 0.013 | Tier II |
18 | NC_000012.12 | 12 | 62143509 | - | TAFA2 | NNNCTGGACAGGTTTTATAG | NTG | 2 | 0.012 | Tier II |
19 | NC_000009.12 | 9 | 127729124 | + | TTC16 | NNNCTGGACAGGTTGCTTAG | NGG | 2 | 0.0067 | Tier II |
20 | NC_000014.9 | 14 | 79625690 | - | LOC105370583 | NNNCTGGAGAGGTTTCATAG | NGG | 1 | 0.619 | Tier III |
21 | NC_000007.14 | 7 | 64226515 | + | LOC105375319 | NNNCTAGACAGGTTTCACAG | NGG | 2 | 0.4286 | Tier III |
22 | NC_000005.10 | 5 | 121840951 | - | LOC105379149 | NNNCTAGACAGGTTTCATAG | NAG | 2 | 0.2593 | Tier III |
23 | NC_000002.12 | 2 | 198612148 | - | LOC105373831 | NNNCTGGAGAGGTTTCAGAG | NGG | 2 | 0.2063 | Tier III |
24 | NC_000009.12 | 9 | 127184465 | - | LOC105376278 | NNNCTGGCCAGGTTTCACAG | NGG | 2 | 0.1837 | Tier III |
25 | NC_000004.12 | 4 | 54336014 | + | LINC02283 | NNNCTGGACAAGTTTCCTAG | NGG | 2 | 0.1765 | Tier III |
26 | NC_000015.10 | 15 | 69669977 | - | PCAT29 | NNNCAGGACAGGTTTCCTAG | NGG | 2 | 0.0882 | Tier III |
27 | NC_000003.12 | 3 | 181347649 | - | SOX2-OT | NNNGTGGACAGGTTTGATAG | NGG | 2 | 0.0769 | Tier III |
28 | NC_000005.10 | 5 | 164540715 | + | LOC102546299 | NNNCTGGACAGGTGTCCTAG | NGG | 2 | 0.0 | Tier III |
29 | NC_000005.10 | 5 | 164540715 | + | LINC03000 | NNNCTGGACAGGTGTCCTAG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000071.6 | 5 | 36331194 | + | Sorcs2 | NNNCTGGACAGGCTTCATAA | NGG | 2 | 0.7401 | Tier II |
2 | NC_000067.6 | 1 | 132711490 | - | Nfasc | NNNCTGGACAGACTTCATAG | NGG | 2 | 0.7368 | Tier II |
3 | NC_000067.6 | 1 | 32178877 | - | Khdrbs2 | NNNCTGAACAGCTTTCATAG | NGG | 2 | 0.5294 | Tier II |
4 | NC_000076.6 | 10 | 89319579 | + | Ano4 | NNNCTGGACAATTTTCATAG | NGG | 2 | 0.3846 | Tier II |
5 | NC_000072.6 | 6 | 29695723 | + | Tspan33 | NNNCTGGAAAGGTTTCACAG | NGG | 2 | 0.3673 | Tier II |
6 | NC_000081.6 | 15 | 88694017 | - | Brd1 | NNNCTGCTCAGGTTTCATAG | NGG | 2 | 0.2946 | Tier II |
7 | NC_000071.6 | 5 | 48984156 | - | Kcnip4 | NNNCTGGCCAGGTTTCATGG | NGG | 2 | 0.1607 | Tier II |
8 | NC_000086.7 | X | 6460703 | + | Shroom4 | NNNCTGGAGAGGTTTCATAG | NAG | 2 | 0.1605 | Tier II |
9 | NC_000085.6 | 19 | 53797959 | + | Rbm20 | NNNCTGGACAGGTCTCACAG | NGG | 2 | 0.1224 | Tier II |
10 | NC_000077.6 | 11 | 35289804 | + | Slit3 | NNNCTGGACAGGTCTCAGAG | NGG | 2 | 0.0952 | Tier II |
11 | NC_000072.6 | 6 | 129401218 | - | Clec1b | NNNCTGGACAGGTTTCTAAG | NGG | 2 | 0.0889 | Tier II |
12 | NC_000072.6 | 6 | 129401218 | - | Clec12b | NNNCTGGACAGGTTTCTAAG | NGG | 2 | 0.0889 | Tier II |
13 | NC_000080.6 | 14 | 48513700 | + | Tmem260 | NNNCTGGACAGGGTTCAGAG | NGG | 2 | 0.087 | Tier II |
14 | NC_000085.6 | 19 | 57928963 | + | Atrnl1 | NNNCTGGACAGGTTTTATAG | NAG | 2 | 0.0798 | Tier II |
15 | NC_000069.6 | 3 | 138763678 | + | Tspan5 | NNNCTGGTCAGGTTTCTTAG | NGG | 2 | 0.0571 | Tier II |
16 | NC_000085.6 | 19 | 41503606 | + | Lcor | NNNCTGGACAGGTTTCATAA | NTG | 2 | 0.0365 | Tier II |
17 | NC_000084.6 | 18 | 21272595 | - | Garem1 | NNNCTGGAGAGGTTTCATAG | NGC | 2 | 0.0138 | Tier II |
18 | NC_000071.6 | 5 | 26859480 | - | Dpp6 | NNNCTGGACAGCTTTCATAG | NGC | 2 | 0.0118 | Tier II |
19 | NC_000067.6 | 1 | 88086151 | + | Ugt1a10 | NNNCTGGACAGGTTCCATAG | NTG | 2 | 0.0106 | Tier II |
20 | NC_000067.6 | 1 | 88086151 | + | Ugt1a9 | NNNCTGGACAGGTTCCATAG | NTG | 2 | 0.0106 | Tier II |
21 | NC_000071.6 | 5 | 23054305 | + | Lhfpl3 | NNNCTGGACAGCTTTCATAG | NGT | 2 | 0.0085 | Tier II |
22 | NC_000086.7 | X | 6460703 | + | 5430428K19Rik | NNNCTGGAGAGGTTTCATAG | NAG | 2 | 0.1605 | Tier III |
23 | NC_000086.7 | X | 8002177 | - | Gm39488 | NNNCTGGACAGGTTTTATGG | NGG | 2 | 0.1154 | Tier III |
24 | NC_000077.6 | 11 | 35289804 | + | Gm46277 | NNNCTGGACAGGTCTCAGAG | NGG | 2 | 0.0952 | Tier III |