Construct: sgRNA BRDN0001487138

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGCGCTCTGGTTGCATCCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80227

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 193301281 - ATP13A5 NNNGCTCTGGTTTCATCCTT NGG 2 0.1385 Tier I
2 NC_000020.11 20 46949831 + EYA2 NNNGCTTTGGTTGCAACCCT NGG 2 0.7386 Tier II
3 NC_000001.11 1 108881026 + GPSM2 NNNGCTCTGATTGCATACCT NGG 2 0.4356 Tier II
4 NC_000022.11 22 47066522 + TBC1D22A NNNCCTCTGTTTGCATCCCT NGG 2 0.4301 Tier II
5 NC_000001.11 1 2088424 + PRKCZ NNNGCCCTGGTTGCATCCTT NGG 2 0.4196 Tier II
6 NC_000001.11 1 7676199 - CAMTA1 NNNGCTCTGTTGGCATCCCT NGG 2 0.4063 Tier II
7 NC_000005.10 5 176528310 + RNF44 NNNGGTCTGGCTGCATCCCT NGG 2 0.4 Tier II
8 NC_000017.11 17 74338254 + KIF19 NNNGTTCTGGTTGCATCCCA NGG 2 0.358 Tier II
9 NC_000001.11 1 231028164 + FAM89A NNNCCTCTGGCTGCATCCCT NGG 2 0.3529 Tier II
10 NC_000017.11 17 20949507 + LOC124900389 NNNGCTCTGTTTGCATCCAT NGG 2 0.3482 Tier II
11 NC_000002.12 2 73092927 - RAB11FIP5 NNNGCTCTGGTTGAGTCCCT NGG 2 0.2275 Tier II
12 NC_000017.11 17 45672702 + LINC02210-CRHR1 NNNGCTCTGGTTGCTTCCTT NGG 2 0.0923 Tier II
13 NC_000005.10 5 78542473 + LHFPL2 NNNGCTCTGCTTGCATCCCG NGG 2 0.0706 Tier II
14 NC_000005.10 5 42649942 - GHR NNNGCTCTGGTTTCTTCCCT NGG 2 0.06 Tier II
15 NC_000006.12 6 13974242 - RNF182 NNNGCTCTGGTAGCATCCCT NGA 2 0.0556 Tier II
16 NC_000008.11 8 23399079 + LOXL2 NNNGCTCTGGTTGCATCTCT NTG 2 0.025 Tier II
17 NC_000019.10 19 17645630 + UNC13A NNNGCTCTGGCTGGATCCCT NGG 2 0.0 Tier II
18 NC_000017.11 17 74338254 + LOC107985078 NNNGTTCTGGTTGCATCCCA NGG 2 0.358 Tier III
19 NC_000017.11 17 20949507 + LOC339260 NNNGCTCTGTTTGCATCCAT NGG 2 0.3482 Tier III
20 NC_000003.12 3 13680373 + LINC00620 NNNGCTCAGGTTGCTTCCCT NGG 2 0.16 Tier III
21 NC_000017.11 17 6865214 + ALOX12P2 NNNGCTCTGGTTCCATCCCT NGA 2 0.0292 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 135998580 - Igfn1 NNNGCTCTGGTGACATCCCT NGG 2 0.4615 Tier I
2 NC_000085.6 19 6994749 - Nudt22 NNNGCTCTGGTGGCATCCCC NGG 2 0.0455 Tier I
3 NC_000068.7 2 7389408 - Celf2 NNNGCTCTGGTTATATCCCT NGG 2 0.6769 Tier II
4 NC_000081.6 15 11877492 - Npr3 NNNGCTCTGGCAGCATCCCT NGG 2 0.5333 Tier II
5 NC_000069.6 3 8518126 + Stmn2 NNNGCACTGGTTGCATTCCT NGG 2 0.4044 Tier II
6 NC_000076.6 10 85408243 - Btbd11 NNNGCTCTCGTTGTATCCCT NGG 2 0.3949 Tier II
7 NC_000067.6 1 91025034 - Lrrfip1 NNNGATCTGGCTGCATCCCT NGG 2 0.381 Tier II
8 NC_000070.6 4 147919045 - Plod1 NNNTCTCTGATTGCATCCCT NGG 2 0.3394 Tier II
9 NC_000084.6 18 25102159 + Fhod3 NNNGCCCTGGTTTCATCCCT NGG 2 0.2727 Tier II
10 NC_000077.6 11 85358128 + Bcas3 NNNGCTCTGGGTCCATCCCT NGG 2 0.1684 Tier II
11 NC_000077.6 11 32667115 + Fbxw11 NNNGCTCTGGTTGCATCTCT NGC 2 0.0143 Tier II
12 NC_000067.6 1 193158589 + Irf6 NNNGGTCTGGTTGCATCCCT NGC 2 0.0133 Tier II
13 NC_000079.6 13 111795964 - Map3k1 NNNGCTCTGGTTCCATCCCT NGC 2 0.0094 Tier II
14 NC_000081.6 15 35408530 - Vps13b NNNGCTCTGGCTGGATCCCT NGG 2 0.0 Tier II
15 NC_000071.6 5 89539118 - Npffr2 NNNGCTCTGGTTGCATCCCT NAT 2 0.0 Tier II
16 NC_000084.6 18 23633178 + Dtna NNNGCTCTGGTTGGAACCCT NGG 2 0.0 Tier II
17 NC_000069.6 3 153086429 + Gm52620 NNNGCTCTGGTTACATCCAT NGG 2 0.3956 Tier III
18 NC_000083.6 17 39896395 - AY036118 NNNGCTCTGGTTGCAGACCT NGG 2 0.1615 Tier III
19 NC_000070.6 4 95105954 - Junos NNNGCTCTGGTTGCATCCAT NAG 2 0.1111 Tier III
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Other clones with same target sequence:

(none)