Construct: sgRNA BRDN0001487146
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCACAAACTGAATTACGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TNNI3K (51086)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77896
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
74369479 |
- |
TNNI3K |
NNNACAAACTGAATTACGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
74369479 |
- |
FPGT-TNNI3K |
NNNACAAACTGAATTACGCA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000002.12 |
2 |
110970286 |
+ |
ACOXL |
NNNACAAAATGAATTACACA |
NGG |
2 |
0.5934 |
Tier II |
| 4 |
NC_000005.10 |
5 |
113372408 |
+ |
MCC |
NNNACATACTGAATTACACA |
NGG |
2 |
0.3029 |
Tier II |
| 5 |
NC_000016.10 |
16 |
14108458 |
+ |
MRTFB |
NNNACATACTGAATTATGCA |
NGG |
2 |
0.2042 |
Tier II |
| 6 |
NC_000023.11 |
X |
31478807 |
+ |
DMD |
NNNACAAACTGATTTATGCA |
NGG |
2 |
0.14 |
Tier II |
| 7 |
NC_000009.12 |
9 |
110745086 |
+ |
MUSK |
NNNACAAACTGAATCAAGCA |
NGG |
2 |
0.1273 |
Tier II |
| 8 |
NC_000003.12 |
3 |
152461887 |
+ |
MBNL1 |
NNNACAAACTGAATTACCCA |
NAG |
2 |
0.1235 |
Tier II |
| 9 |
NC_000003.12 |
3 |
10363163 |
- |
ATP2B2 |
NNNACAAACTGAATTACGGA |
NGA |
2 |
0.0087 |
Tier II |
| 10 |
NC_000011.10 |
11 |
70418633 |
- |
CTTN |
NNNACAAACTGAAGCACGCA |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
154939690 |
+ |
Tnni3k |
NNNACAAACTGAACCACGCA |
NGG |
2 |
0.0779 |
Tier I |
| 2 |
NC_000067.6 |
1 |
71035447 |
+ |
Bard1 |
NNNACAAAATGAATTACTCA |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000079.6 |
13 |
41043846 |
+ |
Mak |
NNNACAAACAGAATTAAGCA |
NGG |
2 |
0.4 |
Tier II |
| 4 |
NC_000083.6 |
17 |
65691213 |
+ |
Rab31 |
NNNATAAACTGAATTACGCC |
NGG |
2 |
0.1446 |
Tier II |
| 5 |
NC_000080.6 |
14 |
46819616 |
- |
Gmfb |
NNNACAAACTGAATTACACA |
NGT |
2 |
0.0112 |
Tier II |
| 6 |
NC_000072.6 |
6 |
131242548 |
+ |
Klra2 |
NNNACAAACTGAATTTCTCA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000079.6 |
13 |
40716683 |
+ |
Tfap2a |
NNNACAATCTGAAGTACGCA |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000069.6 |
3 |
131493365 |
+ |
Gm36753 |
NNNACAAACTGATTAACGCA |
NGG |
2 |
0.1737 |
Tier III |
| 9 |
NC_000078.6 |
12 |
33021541 |
- |
Gm40874 |
NNNACAAACTGCATTAAGCA |
NGG |
2 |
0.1228 |
Tier III |
| 10 |
NC_000072.6 |
6 |
16574655 |
- |
Gm36503 |
NNNACAAACTGAATAAGGCA |
NGG |
2 |
0.0341 |
Tier III |
Other clones with same target sequence:
(none)
