Construct: sgRNA BRDN0001487148
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGTGTAAATCGGCCAGCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FN3KRP (79672)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78004
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
82720298 |
- |
FN3KRP |
NNNGTGTAAATCGGCCAGCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
123755624 |
- |
ATP6V0A2 |
NNNGTGTAAATTGGCAAGCT |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000004.12 |
4 |
150225041 |
- |
DCLK2 |
NNNGTGTAAATCATCCAGCT |
NGG |
2 |
0.2462 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
56775740 |
- |
Lingo1 |
NNNGTGTTAATCGCCCAGCT |
NGG |
2 |
0.1837 |
Tier II |
| 2 |
NC_000077.6 |
11 |
75372413 |
+ |
Smyd4 |
NNNGTGTATATCTGCCAGCT |
NGG |
2 |
0.18 |
Tier II |
| 3 |
NC_000068.7 |
2 |
6680142 |
- |
Celf2 |
NNNGTGTAAATCTGCCTGCT |
NGG |
2 |
0.04 |
Tier II |
Other clones with same target sequence:
(none)
