Construct: sgRNA BRDN0001487155

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GTAATTGATCTTGTACATGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDR2 (4921)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77975

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 162754788 - DDR2 NNNATTGATCTTGTACATGG NGG 0 1.0 Tier I
2 NC_000022.11 22 24504098 - UPB1 NNNATACATCTTGTACATGG NGG 2 0.5958 Tier II
3 NC_000003.12 3 29514994 - RBMS3 NNNATTGATCTTGAACATAG NGG 2 0.4422 Tier II
4 NC_000007.14 7 49827682 - VWC2 NNNATTGCTCTTGTACATGG NGG 1 0.4286 Tier II
5 NC_000009.12 9 37897519 - SLC25A51 NNNAGTGATCTTTTACATGG NGG 2 0.192 Tier II
6 NC_000012.12 12 103934490 + HSP90B1 NNNATTGCTGTTGTACATGG NGG 2 0.1667 Tier II
7 NC_000017.11 17 67049952 - CACNG1 NNNGTTGATCTTGTACATGG NAG 2 0.162 Tier II
8 NC_000020.11 20 64233520 - MYT1 NNNATTAATCTTGTAGATGG NGG 2 0.1538 Tier II
9 NC_000013.11 13 44514425 - TSC22D1 NNNTTTGATCTTGTTCATGG NGG 2 0.1273 Tier II
10 NC_000003.12 3 112128565 + C3orf52 NNNATTGATCTTGTTCATCG NGG 2 0.0897 Tier II
11 NC_000003.12 3 112128565 + GCSAM NNNATTGATCTTGTTCATCG NGG 2 0.0897 Tier II
12 NC_000003.12 3 105445813 - ALCAM NNNATTGATTTTGTACATGG NGA 2 0.0654 Tier II
13 NC_000003.12 3 141503442 + RASA2 NNNATTGATCTTGTACATAG NGA 2 0.0496 Tier II
14 NC_000004.12 4 99214064 + ADH6 NNNATTGATCTTGTAGATGG NAG 2 0.0399 Tier II
15 NC_000005.10 5 15913454 + FBXL7 NNNATTGATCTTTTACATGG NCG 2 0.0321 Tier II
16 NC_000003.12 3 123956530 - CCDC14 NNNATTCATCTTGTACATGG NTG 2 0.0268 Tier II
17 NC_000023.11 X 10757452 - MID1 NNNATTGATCTTGCACATGG NGA 2 0.0198 Tier II
18 NC_000006.12 6 160085583 + IGF2R NNNATTGATCTTGTACTTGG NTG 2 0.0052 Tier II
19 NC_000003.12 3 157580254 + SLC66A1LP NNNGTTGATCTTGTAAATGG NGG 2 0.625 Tier III
20 NC_000003.12 3 195975378 - SDHAP1 NNNAATGATCTTGTACATCG NGG 2 0.2241 Tier III
21 NC_000006.12 6 15963375 + LOC105374949 NNNCTTTATCTTGTACATGG NGG 2 0.2017 Tier III
22 NC_000009.12 9 37897519 - PAICSP1 NNNAGTGATCTTTTACATGG NGG 2 0.192 Tier III
23 NC_000003.12 3 105445813 - LOC124906263 NNNATTGATTTTGTACATGG NGA 2 0.0654 Tier III
24 NC_000004.12 4 99214064 + LOC100507053 NNNATTGATCTTGTAGATGG NAG 2 0.0399 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 170005141 + Ddr2 NNNGTTGATCTTGTACATGG NTG 2 0.0244 Tier I
2 NC_000074.6 8 13281661 + Dcun1d2 NNNATTAATCTAGTACATGG NGG 2 0.8 Tier II
3 NC_000079.6 13 10006799 - Chrm3 NNNATCGATCTTGTACATTG NGG 2 0.6061 Tier II
4 NC_000075.6 9 55749789 - Scaper NNNAGTGATCTTCTACATGG NGG 2 0.2695 Tier II
5 NC_000068.7 2 142256084 - Macrod2 NNNATTTATCTTGTATATGG NGG 2 0.1758 Tier II
6 NC_000069.6 3 64266620 - Vmn2r3 NNNATTGATCTTGAACATGG NAG 2 0.1605 Tier II
7 NC_000084.6 18 57559194 - Ccdc192 NNNATTTATCTTGTACGTGG NGG 2 0.1008 Tier II
8 NC_000070.6 4 4065451 + Sdr16c6 NNNATTGATCTTGTACTTTG NGG 2 0.0889 Tier II
9 NC_000078.6 12 32850753 - Nampt NNNATTAATCTTGTACATGG NTG 2 0.039 Tier II
10 NC_000075.6 9 63502297 + Iqch NNNATTGATCTAGTACATGG NGC 2 0.0178 Tier II
11 NC_000078.6 12 91641421 - Ston2 NNNATTGATCTTGGAAATGG NGG 2 0.0 Tier II
12 NC_000075.6 9 63502297 + Gm16759 NNNATTGATCTAGTACATGG NGC 2 0.0178 Tier III
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Other clones with same target sequence:

(none)