Construct: sgRNA BRDN0001487156

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GAAGATGTCAACGCCCATCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK1E (1454)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77976

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000022.11 22 38299922 - CSNK1E NNNGATGTCAACGCCCATCG NGG 0 1.0 Tier I
2 NC_000022.11 22 38299922 - TPTEP2-CSNK1E NNNGATGTCAACGCCCATCG NGG 0 1.0 Tier I
3 NC_000004.12 4 1872483 - NSD2 NNNAATGTCAAAGCCCATCG NGG 2 0.6429 Tier II
4 NC_000007.14 7 26121263 + LOC105375198 NNNGATGTCAAGGCTCATCG NGG 2 0.0296 Tier III
5 NC_000007.14 7 26121263 + LOC105375199 NNNGATGTCAAGGCTCATCG NGG 2 0.0296 Tier III
6 NC_000017.11 17 36893749 - LHX1-DT NNNGATGTCAATGGCCATCG NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 79425527 - Csnk1e NNNGATGTCAACGCCAATCG NGG 1 1.0 Tier I
Download CSV

Other clones with same target sequence:

(none)