Construct: sgRNA BRDN0001487158
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTACTTACACACTCGCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GRK3 (157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77943
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
25690276 |
- |
GRK3 |
NNNACTTACACACTCGCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
50291406 |
- |
PLXNB2 |
NNNAGTTACACACACGCATG |
NGG |
2 |
0.3714 |
Tier II |
| 3 |
NC_000012.12 |
12 |
23750896 |
+ |
SOX5 |
NNNATTTACACACTGGCATG |
NGG |
2 |
0.0318 |
Tier II |
| 4 |
NC_000012.12 |
12 |
128981965 |
+ |
GLT1D1 |
NNNACTTACACACGCGCATG |
NAG |
2 |
0.0 |
Tier II |
| 5 |
NC_000005.10 |
5 |
80198554 |
+ |
SERINC5 |
NNNACTTACACACTCCCATC |
NGG |
2 |
0.0 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
4290082 |
+ |
Grk2 |
NNNACTTACACACTGGCATG |
NGG |
1 |
0.05 |
Tier I |
| 2 |
NC_000076.6 |
10 |
67240424 |
- |
Jmjd1c |
NNNACTTGCACACTCGCATA |
NGG |
2 |
0.6875 |
Tier II |
| 3 |
NC_000080.6 |
14 |
38808325 |
+ |
Nrg3 |
NNNACTTACACACTCACATG |
NAG |
2 |
0.2593 |
Tier II |
| 4 |
NC_000086.7 |
X |
69360307 |
- |
Aff2 |
NNNACTTACACACTCACAGG |
NGG |
2 |
0.25 |
Tier II |
| 5 |
NC_000072.6 |
6 |
29493068 |
- |
Kcp |
NNNACTTACACACACGCGTG |
NGG |
2 |
0.2476 |
Tier II |
| 6 |
NC_000068.7 |
2 |
71482894 |
- |
Metap1d |
NNNACATACACACTCGCATG |
NAG |
2 |
0.2247 |
Tier II |
| 7 |
NC_000068.7 |
2 |
140478006 |
+ |
Macrod2 |
NNNACTTGCACACTGGCATG |
NGG |
2 |
0.0367 |
Tier II |
| 8 |
NC_000079.6 |
13 |
18136471 |
+ |
Pou6f2 |
NNNACTTACACACTCACATG |
NGC |
2 |
0.0222 |
Tier II |
| 9 |
NC_000079.6 |
13 |
23965399 |
- |
Scgn |
NNNACATACACACTCGCATG |
NGC |
2 |
0.0193 |
Tier II |
| 10 |
NC_000085.6 |
19 |
34568477 |
+ |
Ifit2 |
NNNACTTACACACTCACATG |
NGT |
2 |
0.0161 |
Tier II |
| 11 |
NC_000073.6 |
7 |
37607690 |
- |
Zfp536 |
NNNGCTTACACACTCGCATG |
NGT |
2 |
0.0101 |
Tier II |
| 12 |
NC_000079.6 |
13 |
51210966 |
- |
Gm46414 |
NNNACTTACACACTCACATG |
NGC |
2 |
0.0222 |
Tier III |
Other clones with same target sequence:
(none)
