Construct: sgRNA BRDN0001487159
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGTGCTCACTTGAACCACGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LMTK3 (114783)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77993
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
48498509 |
+ |
LMTK3 |
NNNGCTCACTTGAACCACGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
102469640 |
- |
MFSD13A |
NNNAATCACTTGAACCACGA |
NGG |
2 |
0.5143 |
Tier II |
| 3 |
NC_000005.10 |
5 |
145561347 |
- |
PRELID2 |
NNNACTCACTTGAACCAAGA |
NGG |
2 |
0.4846 |
Tier II |
| 4 |
NC_000002.12 |
2 |
74269300 |
- |
SLC4A5 |
NNNGATCACTTGAACCACGG |
NGG |
2 |
0.437 |
Tier II |
| 5 |
NC_000002.12 |
2 |
9408481 |
- |
ITGB1BP1 |
NNNGCTCACTTGAACCAAGG |
NGG |
2 |
0.4118 |
Tier II |
| 6 |
NC_000020.11 |
20 |
48857698 |
+ |
PREX1 |
NNNGATCACTTGAACCAAGA |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000007.14 |
7 |
150314877 |
+ |
LRRC61 |
NNNGCTCACTTGAACCCCGG |
NGG |
2 |
0.1349 |
Tier II |
| 8 |
NC_000007.14 |
7 |
150314877 |
+ |
ACTR3C |
NNNGCTCACTTGAACCCCGG |
NGG |
2 |
0.1349 |
Tier II |
| 9 |
NC_000010.11 |
10 |
101447245 |
- |
BTRC |
NNNGGTCACTTGACCCACGA |
NGG |
2 |
0.1286 |
Tier II |
| 10 |
NC_000001.11 |
1 |
149980637 |
+ |
OTUD7B |
NNNGATCACTTGAACCAGGA |
NGG |
2 |
0.0762 |
Tier II |
| 11 |
NC_000001.11 |
1 |
153950026 |
- |
CRTC2 |
NNNGATCACTTGAACCAGGA |
NGG |
2 |
0.0762 |
Tier II |
| 12 |
NC_000002.12 |
2 |
231202416 |
- |
ARMC9 |
NNNGATCACTTGAACCAGGA |
NGG |
2 |
0.0762 |
Tier II |
| 13 |
NC_000010.11 |
10 |
102469640 |
- |
LOC124902493 |
NNNAATCACTTGAACCACGA |
NGG |
2 |
0.5143 |
Tier III |
| 14 |
NC_000003.12 |
3 |
160048397 |
- |
IL12A-AS1 |
NNNGATCACTTGAACCCCGA |
NGG |
2 |
0.1008 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
78448174 |
+ |
Pdxk |
NNNGCTCACTAGAACCAAGA |
NGG |
2 |
0.4038 |
Tier II |
| 2 |
NC_000083.6 |
17 |
56053792 |
+ |
Chaf1a |
NNNGCTCATTTGAACCAGGA |
NGG |
2 |
0.1167 |
Tier II |
| 3 |
NC_000086.7 |
X |
13716479 |
- |
Cask |
NNNGCACACTTGAACCAGGA |
NGG |
2 |
0.1156 |
Tier II |
| 4 |
NC_000072.6 |
6 |
37429705 |
+ |
Creb3l2 |
NNNACTCACTTGAAGCACGA |
NGG |
2 |
0.045 |
Tier II |
| 5 |
NC_000072.6 |
6 |
6328347 |
+ |
Gm20619 |
NNNGCTCACTTGAACCAAGG |
NGG |
2 |
0.4118 |
Tier III |
| 6 |
NC_000079.6 |
13 |
48173321 |
+ |
A330033J07Rik |
NNNGCTCACGTGGACCACGA |
NGG |
2 |
0.3261 |
Tier III |
| 7 |
NC_000070.6 |
4 |
13965579 |
- |
Gm11823 |
NNNGTTCACTTGAATCACGA |
NGG |
2 |
0.0424 |
Tier III |
Other clones with same target sequence:
(none)
