Construct: sgRNA BRDN0001487161

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCGCTTCTCCAGCACCACG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKX (5613)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
78015

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 3655307 - PRKX NNNGCTTCTCCAGCACCACG NGG 0 1.0 Tier I
2 NC_000001.11 1 6455783 - ESPN NNNCCTCCTCCAGCACCACG NGG 2 0.364 Tier I
3 NC_000005.10 5 67166067 - MAST4 NNNGCTTCTCCAGCACCTCC NGG 2 0.2143 Tier I
4 NC_000001.11 1 10665463 + CASZ1 NNNGCTTCTCCACCACCACC NGG 2 0.1805 Tier I
5 NC_000001.11 1 228107735 + MRPL55 NNNGCTTCACCAGCAGCACG NGG 2 0.1429 Tier I
6 NC_000019.10 19 1913109 - ADAT3 NNNGCTCCTCCAGCACCCCG NGG 2 0.131 Tier I
7 NC_000009.12 9 136800004 - CCDC183 NNNGCTTCTCCCGCACCACC NGG 2 0.1128 Tier I
8 NC_000021.9 21 46567466 - DIP2A NNNGCTCCTCCAGCACCACG NTG 2 0.0268 Tier I
9 NC_000007.14 7 1438132 + MICALL2 NNNGCTTCTCCAGCTCCACG NCG 2 0.0214 Tier I
10 NC_000023.11 X 17801640 + RAI2 NNNGCTGCTCCAGCACCACG NGC 2 0.0131 Tier I
11 NC_000005.10 5 139394213 + PROB1 NNNCCTTCTCCAGGACCACG NGG 2 0.0 Tier I
12 NC_000017.11 17 29608089 - ANKRD13B NNNGCTTCTCCAGGAGCACG NGG 2 0.0 Tier I
13 NC_000018.10 18 34872759 + DTNA NNNGCTGCTCCAGCACCACA NGG 2 0.5515 Tier II
14 NC_000017.11 17 75723672 + ITGB4 NNNGCTTCTCCAGCACCTCG NGG 1 0.5 Tier II
15 NC_000010.11 10 79126865 + ZMIZ1 NNNGCTTCCCCAGCACCTCG NGG 2 0.4615 Tier II
16 NC_000009.12 9 98505024 - GABBR2 NNNGGTGCTCCAGCACCACG NGG 2 0.3529 Tier II
17 NC_000006.12 6 24320267 - DCDC2 NNNTCTACTCCAGCACCACG NGG 2 0.3182 Tier II
18 NC_000013.11 13 114123303 + RASA3 NNNGCTGCTCCAGCACCTCG NGG 2 0.2941 Tier II
19 NC_000022.11 22 46151413 - PPARA NNNGCTTCACCCGCACCACG NGG 2 0.2444 Tier II
20 NC_000006.12 6 72856901 - KCNQ5 NNNGCATCTCCAGCCCCACG NGG 2 0.2364 Tier II
21 NC_000011.10 11 12479893 + PARVA NNNGCTTCTCCAGCACCATC NGG 2 0.1978 Tier II
22 NC_000003.12 3 54530360 - CACNA2D3 NNNGCTTTTCCAGCTCCACG NGG 2 0.175 Tier II
23 NC_000019.10 19 1913109 - SCAMP4 NNNGCTCCTCCAGCACCCCG NGG 2 0.131 Tier II
24 NC_000007.14 7 71511551 + GALNT17 NNNGCTTCTCCAGCCCCATG NGG 2 0.1259 Tier II
25 NC_000002.12 2 79863960 - CTNNA2 NNNGCTTCTCCAGCACCAGG NGG 1 0.125 Tier II
26 NC_000009.12 9 133701349 + SARDH NNNGCTTCTCCAGCCCCACC NGG 2 0.1169 Tier II
27 NC_000018.10 18 4209192 - DLGAP1 NNNGCTTCTCCAGCACCACA NTG 2 0.0365 Tier II
28 NC_000005.10 5 139394213 + SPATA24 NNNCCTTCTCCAGGACCACG NGG 2 0.0 Tier II
29 NC_000012.12 12 120093414 + BICDL1 NNNGCTTCTCCAGGACCACG NGC 2 0.0 Tier II
30 NC_000024.10 Y 7326012 + PRKY NNNACTTCTCCAGCACCACG NGG 1 0.9 Tier III
31 NC_000009.12 9 119874673 + LOC107987122 NNNGCCTCTCCAGCACCACA NGG 2 0.8523 Tier III
32 NC_000015.10 15 35774904 - DPH6-DT NNNGCTTCTCCAACACCACC NGG 2 0.3956 Tier III
33 NC_000001.11 1 16692718 + ESPNP NNNCCTCCTCCAGCACCACG NGG 2 0.364 Tier III
34 NT_187369.1 1 18542 + LOC101060324 NNNCCTCCTCCAGCACCACG NGG 2 0.364 Tier III
35 NC_000010.11 10 121032884 + LOC105378521 NNNGCTTCCCCAGAACCACG NGG 2 0.3231 Tier III
36 NC_000010.11 10 11690030 - LOC105376413 NNNGCTCCTCCAGAACCACG NGG 2 0.2406 Tier III
37 NC_000018.10 18 22348881 - LOC105372019 NNNGCTTCTCCAGCGCCAGG NGG 2 0.0813 Tier III
38 NC_000014.9 14 97542967 - LINC02325 NNNGCTTCTCCAGCAGCTCG NGG 2 0.0769 Tier III
39 NC_000008.11 8 95489282 - CFAP418-AS1 NNNGCTTCTCCAGCACCACT NTG 2 0.0273 Tier III
40 NC_000014.9 14 23701035 - LOC105370409 NNNGCTTCTCCAGCACCATG NGC 2 0.0103 Tier III
41 NC_000014.9 14 23701035 - LOC105370410 NNNGCTTCTCCAGCACCATG NGC 2 0.0103 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 39047995 - Lama4 NNNGCTGCTCCAGCACCACG NGG 1 0.5882 Tier I
2 NC_000069.6 3 94425917 - Tdrkh NNNGCTTCTCCAGCTCCACC NGG 2 0.0857 Tier I
3 NC_000073.6 7 26308569 + Cyp2a4 NNNGCTTCTCCATCACCACG NTG 2 0.0117 Tier I
4 NC_000083.6 17 56288056 - Plin3 NNNACTTCTCCAGCACCACA NGG 2 0.8438 Tier II
5 NC_000078.6 12 51756844 + Hectd1 NNNGCTTCTCCAGCACCACT NGG 1 0.7 Tier II
6 NC_000078.6 12 34150480 + Hdac9 NNNCCTTCTCCAGCACCACA NGG 2 0.4963 Tier II
7 NC_000071.6 5 106685133 + Zfp644 NNNACTTCTCCAGCACCATG NGG 2 0.4154 Tier II
8 NC_000075.6 9 64124681 - Lctl NNNACTTCTCCAGCACCATG NGG 2 0.4154 Tier II
9 NC_000067.6 1 151603636 - Niban1 NNNTCTTCTCCAGCACCACG NGG 1 0.3636 Tier II
10 NC_000072.6 6 45851450 - Cntnap2 NNNGCTTCTCCAGTACCAAG NGG 2 0.3143 Tier II
11 NC_000085.6 19 59344414 - Pdzd8 NNNGCATCTCCTGCACCACG NGG 2 0.2889 Tier II
12 NC_000076.6 10 123036966 - Mon2 NNNGCTTCTCCAACATCACG NGG 2 0.284 Tier II
13 NC_000070.6 4 122840197 + Ppt1 NNNACTTCTCCAGCCCCACG NGG 2 0.2455 Tier II
14 NC_000073.6 7 129266573 - Plpp4 NNNCCTTCTCCAGCACCATG NGG 2 0.2443 Tier II
15 NC_000083.6 17 84997801 - Ppm1b NNNCCTTCTCCAGCACCATG NGG 2 0.2443 Tier II
16 NC_000085.6 19 30812029 + Prkg1 NNNCCTTCTCCAGCACCATG NGG 2 0.2443 Tier II
17 NC_000083.6 17 56868702 + Acsbg2 NNNGCATCTCCAGCCCCACG NGG 2 0.2364 Tier II
18 NC_000082.6 16 38001173 - Gpr156 NNNCCTTCTCTAGCACCACG NGG 2 0.1629 Tier II
19 NC_000069.6 3 28287922 - Tnik NNNGCTTCTCCATCACCTCG NGG 2 0.15 Tier II
20 NC_000073.6 7 99907808 - Xrra1 NNNTCTTCTCCAGCACCCCG NGG 2 0.0693 Tier II
21 NC_000083.6 17 32488090 - Cyp4f39 NNNCCTTCTCCAGCACCACG NCG 2 0.0567 Tier II
22 NC_000085.6 19 53783984 - Rbm20 NNNGCTTCTCCAGCACCATG NCG 2 0.0495 Tier II
23 NC_000070.6 4 125518420 - Grik3 NNNCCTTCTCCAGCACCACG NGA 2 0.0368 Tier II
24 NC_000078.6 12 50556556 + Prkd1 NNNGCTCCTCCAGCACCACG NTG 2 0.0268 Tier II
25 NC_000070.6 4 99100004 + Dock7 NNNGCTTCTCCAGCATGACG NGG 2 0.0181 Tier II
26 NC_000076.6 10 93479731 - Lta4h NNNGCTTCTCCAGCACCAGG NGA 2 0.0087 Tier II
27 NC_000069.6 3 135490716 + Manba NNNCCTTCTCCAGCACCACG NGT 2 0.0085 Tier II
28 NC_000075.6 9 105241457 - Nek11 NNNCCTTCTCCAGCACCACG NGT 2 0.0085 Tier II
29 NC_000079.6 13 12459996 + Lgals8 NNNGCTTCTCCAGCACCACG NCT 2 0.0 Tier II
30 NC_000072.6 6 39255095 + Gm34147 NNNGCTTCTCCAACACCAAG NGG 2 0.3956 Tier III
31 NC_000078.6 12 54100153 + Gm35818 NNNGCTTCTCCAGCACCATG NAG 2 0.1197 Tier III
32 NC_000085.6 19 53783984 - Gm30890 NNNGCTTCTCCAGCACCATG NCG 2 0.0495 Tier III
33 NC_000076.6 10 118550357 - Ifngas1 NNNGCTTCTCCAGCACCACG NGT 1 0.0161 Tier III
34 NC_000073.6 7 34532274 - Gm35297 NNNGCTTCTCCAGCACCATG NGT 2 0.0074 Tier III
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Other clones with same target sequence:

(none)