Construct: sgRNA BRDN0001487162
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGTAACCGCAGTTCTCTGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK14 (1432)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77925
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
36052791 |
- |
MAPK14 |
NNNTAACCGCAGTTCTCTGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
174898660 |
+ |
RABGAP1L |
NNNTAACAGCAGTTCTCTGT |
NGG |
1 |
0.65 |
Tier II |
| 3 |
NC_000001.11 |
1 |
203134752 |
- |
ADORA1 |
NNNTAACGGTAGTTCTCTGT |
NGG |
2 |
0.605 |
Tier II |
| 4 |
NC_000010.11 |
10 |
22414251 |
+ |
SPAG6 |
NNNTAACCCCAATTCTCTGT |
NGG |
2 |
0.5026 |
Tier II |
| 5 |
NC_000017.11 |
17 |
34582566 |
+ |
TMEM132E |
NNNTAACCTCAGTTCTCTAT |
NGG |
2 |
0.381 |
Tier II |
| 6 |
NC_000002.12 |
2 |
58075951 |
+ |
VRK2 |
NNNTAACCTCAGTTCTCTGA |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000005.10 |
5 |
143003301 |
- |
ARHGAP26 |
NNNTTACCGCAGTTCTCTGT |
NAG |
2 |
0.0943 |
Tier II |
| 8 |
NC_000001.11 |
1 |
217190153 |
+ |
LOC105372920 |
NNNTATCCCCAGTTCTCTGT |
NGG |
2 |
0.3846 |
Tier III |
| 9 |
NC_000002.12 |
2 |
194921005 |
- |
LOC105376755 |
NNNTAACCTCAGTTCTCTGA |
NGG |
2 |
0.3 |
Tier III |
| 10 |
NC_000021.9 |
21 |
36153731 |
- |
CBR3-AS1 |
NNNTAACCACTGTTCTCTGT |
NGG |
2 |
0.1978 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000069.6 |
3 |
105914701 |
+ |
Tmigd3 |
NNNTAACTGCAGATCTCTGT |
NGG |
2 |
0.6058 |
Tier II |
| 2 |
NC_000068.7 |
2 |
115735298 |
- |
BC052040 |
NNNTAACCTTAGTTCTCTGT |
NGG |
2 |
0.502 |
Tier II |
| 3 |
NC_000068.7 |
2 |
102896687 |
+ |
Cd44 |
NNNTAACAGCAGTTCTCTAT |
NGG |
2 |
0.4643 |
Tier II |
| 4 |
NC_000077.6 |
11 |
87395655 |
+ |
Rad51c |
NNNTAACCCCAGCTCTCTGT |
NGG |
2 |
0.4251 |
Tier II |
| 5 |
NC_000079.6 |
13 |
83609506 |
- |
Mef2c |
NNNTAACATCAGTTCTCTGT |
NGG |
2 |
0.3467 |
Tier II |
| 6 |
NC_000083.6 |
17 |
69265353 |
+ |
Epb41l3 |
NNNGAACCTCAGTTCTCTGT |
NGG |
2 |
0.3333 |
Tier II |
| 7 |
NC_000068.7 |
2 |
154475187 |
- |
Cbfa2t2 |
NNNTAACCCCAGTACTCTGT |
NGG |
2 |
0.3333 |
Tier II |
| 8 |
NC_000070.6 |
4 |
139315496 |
+ |
Akr7a5 |
NNNTCACCGCAGTTCCCTGT |
NGG |
2 |
0.3333 |
Tier II |
| 9 |
NC_000071.6 |
5 |
105712940 |
- |
Lrrc8d |
NNNTAACCGCAGCCCTCTGT |
NGG |
2 |
0.2256 |
Tier II |
| 10 |
NC_000076.6 |
10 |
50912040 |
+ |
Sim1 |
NNNTAACCTCTGTTCTCTGT |
NGG |
2 |
0.1641 |
Tier II |
| 11 |
NC_000072.6 |
6 |
39952421 |
- |
Tmem178b |
NNNTAACCGCAGTTCTCTGT |
NGA |
1 |
0.0694 |
Tier II |
| 12 |
NC_000077.6 |
11 |
20699361 |
- |
Aftph |
NNNTAACCCCAGTTCTCTGC |
NGG |
2 |
0.049 |
Tier II |
| 13 |
NC_000071.6 |
5 |
93123462 |
+ |
Septin11 |
NNNTAAACGCAGTTCTGTGT |
NGG |
2 |
0.0441 |
Tier II |
| 14 |
NC_000072.6 |
6 |
142356780 |
+ |
Recql |
NNNTAACCGCAGTTCTCTGT |
NGT |
1 |
0.0161 |
Tier II |
| 15 |
NC_000072.6 |
6 |
142356780 |
+ |
Pyroxd1 |
NNNTAACCGCAGTTCTCTGT |
NGT |
1 |
0.0161 |
Tier II |
| 16 |
NC_000083.6 |
17 |
3143236 |
+ |
Scaf8 |
NNNTAACCGCAGTTCTCTAT |
NGT |
2 |
0.0115 |
Tier II |
| 17 |
NC_000079.6 |
13 |
83609506 |
- |
Gm52043 |
NNNTAACATCAGTTCTCTGT |
NGG |
2 |
0.3467 |
Tier III |
| 18 |
NC_000079.6 |
13 |
37531781 |
- |
Gm30863 |
NNNTAACCACAGTTCTCTCT |
NGG |
2 |
0.2882 |
Tier III |
| 19 |
NC_000075.6 |
9 |
70158315 |
+ |
Gm39476 |
NNNTAACTGCAGTTCTCTGT |
NGA |
2 |
0.0608 |
Tier III |
Other clones with same target sequence:
(none)
