Construct: shRNA TRCN0000000005

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001356.x-2627s1c1
DNA Barcode:
AGCAGATTTAGTGGAGGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX3X (1654)
Gene Description:
DEAD-box helicase 3 X-linked
Transcript:
RefSeq NM_001356.2 (NON-CURRENT)
Match location:
Position 2627 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000005)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAGATTTAGTGGAGGGTTT
Hairpin Sequence:
5'-CCGG-AGCAGATTTAGTGGAGGGTTT-CTCGAG-AAACCCTCCACTAAATCTGCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAGATTTAGTGGAGGGTTTCTCGAGAAACCCTCCACTAAATCTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAGATTTAGTGGAGGGTTTCTCGAGAAACCCTCCACTAAATCTGCT-3'

Other clones with same target sequence:

(none)