Construct: shRNA TRCN0000000139

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005869.x-1153s1c1
DNA Barcode:
ACGGGAACTCTTAGCAGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CWC27 (10283)
Gene Description:
CWC27 spliceosome associated cyclophilin
Transcript:
RefSeq NM_005869.1 (NON-CURRENT)
Match location:
Position 1153 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000139)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGGAACTCTTAGCAGCAAA
Hairpin Sequence:
5'-CCGG-ACGGGAACTCTTAGCAGCAAA-CTCGAG-TTTGCTGCTAAGAGTTCCCGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGGAACTCTTAGCAGCAAACTCGAGTTTGCTGCTAAGAGTTCCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGGAACTCTTAGCAGCAAACTCGAGTTTGCTGCTAAGAGTTCCCGT-3'

Other clones with same target sequence:

  1. TRCN0000338376