Construct: shRNA TRCN0000000145

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007006.x-743s1c1
DNA Barcode:
CAGTGTAGAATAAATGTGGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUDT21 (11051)
Gene Description:
nudix hydrolase 21
Transcript:
RefSeq NM_007006.1 (NON-CURRENT)
Match location:
Position 743 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000145)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGTAGAATAAATGTGGTA
Hairpin Sequence:
5'-CCGG-CAGTGTAGAATAAATGTGGTA-CTCGAG-TACCACATTTATTCTACACTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGTAGAATAAATGTGGTACTCGAGTACCACATTTATTCTACACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGTAGAATAAATGTGGTACTCGAGTACCACATTTATTCTACACTG-3'

Other clones with same target sequence:

  1. TRCN0000272572