Construct: shRNA TRCN0000000150

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007007.x-2697s1c1
DNA Barcode:
GCAGCAGGTAAATGTGAGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPSF6 (11052)
Gene Description:
cleavage and polyadenylation specific factor 6
Transcript:
RefSeq NM_007007.1 (NON-CURRENT)
Match location:
Position 2697 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000150)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAGGTAAATGTGAGTAA
Hairpin Sequence:
5'-CCGG-GCAGCAGGTAAATGTGAGTAA-CTCGAG-TTACTCACATTTACCTGCTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAGGTAAATGTGAGTAACTCGAGTTACTCACATTTACCTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAGGTAAATGTGAGTAACTCGAGTTACTCACATTTACCTGCTGC-3'

Other clones with same target sequence:

(none)