Construct: shRNA TRCN0000000167

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015342.x-1358s1c1
DNA Barcode:
CCAAACGAGAACCAGAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPWD1 (23398)
Gene Description:
peptidylprolyl isomerase domain and WD repeat containing 1
Transcript:
RefSeq NM_015342.1 (NON-CURRENT)
Match location:
Position 1358 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000167)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACGAGAACCAGAAGATA
Hairpin Sequence:
5'-CCGG-CCAAACGAGAACCAGAAGATA-CTCGAG-TATCTTCTGGTTCTCGTTTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACGAGAACCAGAAGATACTCGAGTATCTTCTGGTTCTCGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACGAGAACCAGAAGATACTCGAGTATCTTCTGGTTCTCGTTTGG-3'

Other clones with same target sequence:

(none)