Construct: shRNA TRCN0000000177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016333.x-2974s1c1
DNA Barcode:
CCCAAAGTGAAGGCAATAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRRM2 (23524)
Gene Description:
serine/arginine repetitive matrix 2
Transcript:
RefSeq NM_016333.2 (NON-CURRENT)
Match location:
Position 2974 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAAGTGAAGGCAATAATA
Hairpin Sequence:
5'-CCGG-CCCAAAGTGAAGGCAATAATA-CTCGAG-TATTATTGCCTTCACTTTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAAGTGAAGGCAATAATACTCGAGTATTATTGCCTTCACTTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAAGTGAAGGCAATAATACTCGAGTATTATTGCCTTCACTTTGGG-3'

Other clones with same target sequence:

  1. TRCN0000314562