Construct: shRNA TRCN0000000213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002247.x-1789s1c1
DNA Barcode:
CCGAGCTGGAATTGGAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNMA1 (3778)
Gene Description:
potassium calcium-activated channel subfamily M alpha 1
Transcript:
RefSeq NM_002247.2 (NON-CURRENT)
Match location:
Position 1789 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCTGGAATTGGAAAGAA
Hairpin Sequence:
5'-CCGG-CCGAGCTGGAATTGGAAAGAA-CTCGAG-TTCTTTCCAATTCCAGCTCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCTGGAATTGGAAAGAACTCGAGTTCTTTCCAATTCCAGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCTGGAATTGGAAAGAACTCGAGTTCTTTCCAATTCCAGCTCGG-3'

Other clones with same target sequence:

(none)