Construct: shRNA TRCN0000000240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004613.x-1781s1c1
DNA Barcode:
AGAAATACCGTGACTGCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TGM2 (7052)
Gene Description:
transglutaminase 2
Transcript:
RefSeq NM_004613.1 (NON-CURRENT)
Match location:
Position 1781 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAATACCGTGACTGCCTTA
Hairpin Sequence:
5'-CCGG-AGAAATACCGTGACTGCCTTA-CTCGAG-TAAGGCAGTCACGGTATTTCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAATACCGTGACTGCCTTACTCGAGTAAGGCAGTCACGGTATTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAATACCGTGACTGCCTTACTCGAGTAAGGCAGTCACGGTATTTCT-3'

Other clones with same target sequence:

(none)