Construct: shRNA TRCN0000000302

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016447.x-528s1c1
DNA Barcode:
GTGGCAGAATTGGTTGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPP6 (51678)
Gene Description:
membrane palmitoylated protein 6
Transcript:
RefSeq NM_016447.2 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000302)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCAGAATTGGTTGGTATA
Hairpin Sequence:
5'-CCGG-GTGGCAGAATTGGTTGGTATA-CTCGAG-TATACCAACCAATTCTGCCAC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCAGAATTGGTTGGTATACTCGAGTATACCAACCAATTCTGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCAGAATTGGTTGGTATACTCGAGTATACCAACCAATTCTGCCAC-3'

Other clones with same target sequence:

  1. TRCN0000273424