Construct: shRNA TRCN0000000316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018371.x-2980s1c1
DNA Barcode:
GACCGCTTTCTTACCTGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSGALNACT1 (55790)
Gene Description:
chondroitin sulfate N-acetylgalactosaminyltransferase 1
Transcript:
RefSeq NM_018371.2 (NON-CURRENT)
Match location:
Position 2980 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCGCTTTCTTACCTGTTTA
Hairpin Sequence:
5'-CCGG-GACCGCTTTCTTACCTGTTTA-CTCGAG-TAAACAGGTAAGAAAGCGGTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCGCTTTCTTACCTGTTTACTCGAGTAAACAGGTAAGAAAGCGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCGCTTTCTTACCTGTTTACTCGAGTAAACAGGTAAGAAAGCGGTC-3'

Other clones with same target sequence:

(none)