Construct: shRNA TRCN0000000407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000455.x-2281s1c1
DNA Barcode:
GAGTGTGCGGTCAATATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK11 (6794)
Gene Description:
serine/threonine kinase 11
Transcript:
RefSeq NM_000455.3 (NON-CURRENT)
Match location:
Position 2281 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGTGCGGTCAATATTTAT
Hairpin Sequence:
5'-CCGG-GAGTGTGCGGTCAATATTTAT-CTCGAG-ATAAATATTGACCGCACACTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGTGCGGTCAATATTTATCTCGAGATAAATATTGACCGCACACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGTGCGGTCAATATTTATCTCGAGATAAATATTGACCGCACACTC-3'

Other clones with same target sequence:

(none)