Construct: shRNA TRCN0000000414

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000459.x-1275s1c1
DNA Barcode:
CGCTACCTACTAATGAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEK (7010)
Gene Description:
TEK receptor tyrosine kinase
Transcript:
RefSeq NM_000459.1 (NON-CURRENT)
Match location:
Position 1275 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000414)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTACCTACTAATGAAGAAA
Hairpin Sequence:
5'-CCGG-CGCTACCTACTAATGAAGAAA-CTCGAG-TTTCTTCATTAGTAGGTAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTACCTACTAATGAAGAAACTCGAGTTTCTTCATTAGTAGGTAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTACCTACTAATGAAGAAACTCGAGTTTCTTCATTAGTAGGTAGCG-3'

Other clones with same target sequence:

(none)