Construct: shRNA TRCN0000000416

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000459.x-520s1c1
DNA Barcode:
CCTACCAGCTACTTTAACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEK (7010)
Gene Description:
TEK receptor tyrosine kinase
Transcript:
RefSeq NM_000459.1 (NON-CURRENT)
Match location:
Position 520 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000416)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCAGCTACTTTAACTAT
Hairpin Sequence:
5'-CCGG-CCTACCAGCTACTTTAACTAT-CTCGAG-ATAGTTAAAGTAGCTGGTAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCAGCTACTTTAACTATCTCGAGATAGTTAAAGTAGCTGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCAGCTACTTTAACTATCTCGAGATAGTTAAAGTAGCTGGTAGG-3'

Other clones with same target sequence:

(none)