Construct: shRNA TRCN0000000442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001105.x-1589s1c1
DNA Barcode:
CGGATGGTGAGCAATGGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1 (90)
Gene Description:
activin A receptor type 1
Transcript:
RefSeq NM_001105.2 (NON-CURRENT)
Match location:
Position 1589 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGGTGAGCAATGGTATA
Hairpin Sequence:
5'-CCGG-CGGATGGTGAGCAATGGTATA-CTCGAG-TATACCATTGCTCACCATCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGGTGAGCAATGGTATACTCGAGTATACCATTGCTCACCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGGTGAGCAATGGTATACTCGAGTATACCATTGCTCACCATCCG-3'

Other clones with same target sequence:

  1. TRCN0000315051