Construct: shRNA TRCN0000000504

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001278.x-2866s1c1
DNA Barcode:
GCATCATAAGGAGTTGGTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHUK (1147)
Gene Description:
component of inhibitor of nuclear factor kappa B kinase complex
Transcript:
RefSeq NM_001278.2 (NON-CURRENT)
Match location:
Position 2866 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000000504)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCATAAGGAGTTGGTGTA
Hairpin Sequence:
5'-CCGG-GCATCATAAGGAGTTGGTGTA-CTCGAG-TACACCAACTCCTTATGATGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCATAAGGAGTTGGTGTACTCGAGTACACCAACTCCTTATGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCATAAGGAGTTGGTGTACTCGAGTACACCAACTCCTTATGATGC-3'

Other clones with same target sequence:

(none)