Construct: shRNA TRCN0000000525
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001396.x-1033s1c1
- DNA Barcode:
- CAGTATATTCAGAGTCGCTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DYRK1A (1859)
- Gene Description:
- dual specificity tyrosine phosphorylation regulated kinase 1A
- Transcript:
- RefSeq NM_001396.2 (NON-CURRENT)
- Match location:
- Position 1033 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000000525)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAGTATATTCAGAGTCGCTTT
- Hairpin Sequence:
-
5'-CCGG-CAGTATATTCAGAGTCGCTTT-CTCGAG-AAAGCGACTCTGAATATACTG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAGTATATTCAGAGTCGCTTTCTCGAGAAAGCGACTCTGAATATACTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAGTATATTCAGAGTCGCTTTCTCGAGAAAGCGACTCTGAATATACTG-3'
Other clones with same target sequence:
-
TRCN0000010612
